An Osmotically Fragile Mutant of Bacillus subtilis with an Active Membrane-Associated Phospholipase A1

  1. Claudia Kent and
  2. W. J. Lennarz
  1. 1Department of Physiological Chemistry, The Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, Maryland 21205

Abstract

By use of a newly developed procedure for the isolation of mutants with osmotically fragile protoplasts, a mutant of Bacillus subtilis was isolated that has a very active enzyme system for the catabolism of phospholipids via the sequential action of a phospholipase A1 (EC 3.1.1.4) and a lysophospholipase (EC 3.1.1.5). The wild-type bacteria contain no detectable phospholipase A1 activity, but do contain a protein that specifically inhibits the phospholipase A1 in the mutant. This protein may play an important role in the control of phospholipid catabolism.

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  1. PNAS October 1, 1972 vol. 69 no. 10 2793-2797
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