Mutants That Produce Nitrogenase in the Presence of Ammonia

  1. Joyce K. Gordon and
  2. Winston J. Brill
  1. Department of Bacteriology, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wis. 53706

Abstract

Mutants of Azotobacter vinelandii that fix N2 in the presence of excess NH4 + have been isolated. A mutant that was unable to synthesize component I and component II of nitrogenase was spontaneously reverted to the N2-fixing phenotype. Of 21 revertants picked, 7 revertants were not as sensitive as the wild type to repression. A derepressed mutant is as sensitive as the wild type to growth inhibition by 2-methylalanine in the presence of glucose.

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  1. PNAS December 1, 1972 vol. 69 no. 12 3501-3503
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