Purification and Properties of D Protein: A Transcription Factor of Escherichia coli

  1. Sudhamoy Ghosh and
  2. Harrison Echols
  1. Department of Molecular Biology, University of California, Berkeley, Calif. 94720

Abstract

D factor is protein that stimulates DNA transcription in vitro by RNA polymerase. For DNA from bacteriophage λ, we present evidence that D protein interacts with DNA to increase the frequency and improve the specificity of initiation by RNA polymerase.

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