Role of Calcium and Adenosine-3′:5′-Cyclic Monophosphate in Controlling Fly Salivary Gland Secretion

  1. William T. Prince,
  2. Michael J. Berridge, and
  3. Howard Rasmussen*
  1. Agricultural Research Council, Unit of Invertebrate Chemistry and Physiology, Department of Zoology, Cambridge University, England
  2. Department of Pharmacology, Cambridge University, England

Abstract

The action of 5-hydroxytryptamine (5-HT) on an insect salivary gland was associated with a rise in adenosine-3′:5′-cyclic monophosphate (cAMP) concentration and an increase in calcium uptake. An increase in secretion induced either by 5-HT or exogenous cAMP required extracellular calcium. Both 5-HT and exogenous cAMP increased 45Ca efflux from previously labeled glands, but only 5-HT caused an increase in calcium uptake. The transepithelial potential in this tissue became more negative after addition of 5-HT, but more positive after addition of cAMP. 5-HT and cAMP induced a more negative potential when calcium was removed from the medium. It was concluded that both calcium and cAMP serve as intracellular messengers when 5-HT acts upon fly salivary gland.

Footnotes

  • ↵ * Present address: Department of Biochemistry, University of Pennsylvania, School of Medicine, Philadelphia, Pa. 19104.

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  1. PNAS March 1, 1972 vol. 69 no. 3 553-557
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