Characterization of Messenger Ribonucleoprotein and Messenger RNA from KB Cells

  1. Ajit Kumar* and
  2. Uno Lindberg
  1. Department of Microbiology, The Wallenberg Laboratory, University of Uppsala, Uppsala, Sweden

Abstract

Messenger ribonucleoprotein and mRNA from KB-cells were isolated under conditions designed to minimize nonspecific RNA-protein interaction and to minimize degradation by contaminating ribonucleases. A large fraction, 60-70%, of the messenger ribonucleoprotein from polysomes dissociated in vitro by either EDTA or puromycin sedimented faster than the large ribosome subunit. Messenger ribonucleoprotein particles with sedimentation coefficients up to 200 S were observed. Released mRNA was also large, with maximal molecular weights around 5 × 106.

Footnotes

  • ↵ * Present address: Harvard Medical School, Massachusetts General Hospital, Boston 14, Mass. 02115

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  1. PNAS March 1, 1972 vol. 69 no. 3 681-685
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