Intracellular Cyclic AMP Concentration Responds Specifically to Growth Regulation by Serum*

  1. Jan Oey†,
  2. Arthur Vogel, and
  3. Robert Pollack‡
  1. 1Cold Spring Harbor Laboratories, Cold Spring Harbor, New York 11724

Abstract

Intracellular concentrations of cyclic AMP increase when cells are deprived of serum. Studies with the mouse fibroblast line 3T3, an SV40-transformed subline of 3T3, and six different revertant lines derived from this clone show that a marked increase in cyclic AMP occurs only when the serum concentration is reduced below the minimum necessary for growth of a given line. Conversely, density-dependent inhibition of growth is not accompanied by an increase in cyclic AMP concentration in any line.

Footnotes

  • ↵ † Present address: University of Konstanz, Germany.

  • ↵ ‡ To whom reprint requests should be sent.

  • ↵ * This is no. V of a series; “Isolation and Characterization of Revertant Cell Lines.” The preceding paper is ref. 7.

« Previous | Next Article »Table of Contents

This Article

  1. PNAS March 1, 1974 vol. 71 no. 3 694-698
  1. » Abstract
  2. Full Text (PDF)

Readers Also Viewed

Link: Advanced Search

This Week's Issue

  1. December 1, 2009, 106 (48)
  1. Current Issue
  1. Alert me to new issues of PNAS

Most