Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library
- J R Duguid,
- R G Rohwer, and
- B Seed
Abstract
We have developed a subtractive cloning procedure based on the hybridization of single-stranded cDNA libraries constructed in pi H3M, a vector containing the phage M13 origin of replication. We have used this strategy to isolate three transcripts whose abundance is increased in scrapie-infected brain. DNA sequence analysis showed that they represent glial fibrillary acidic protein, metallothionein II, and the B chain of alpha-crystallin; the latter two may represent a response to stress.
