Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6 antigen family

  1. M van de Rijn,
  2. S Heimfeld,
  3. G J Spangrude, and
  4. I L Weissman
  1. Department of Pathology, Stanford University School of Medicine, CA 94305.

Abstract

Recently, hematopoietic stem cells were purified to homogeneity from mouse bone marrow. The protein structure of Sca-1, the cell surface antigen used in the isolation of hematopoietic stem cells, is described here. It is shown that the Sca-1 antigen is a member of the Ly-6 antigen family. The anti-Sca-1 antibody was used in immunohistochemistry experiments to define the structures in several tissues that had previously been shown to contain Ly-6 antigens. In thymus, spleen, and kidney, specific staining of parenchymal cells can be demonstrated, whereas only vasculature reacts with anti-Sca-1 in brain, heart, and liver and possibly in lung.

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  1. PNAS June 1, 1989 vol. 86 no. 12 4634-4638
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