Recombinant human bone morphogenetic protein induces bone formation

  1. E A Wang,
  2. V Rosen,
  3. J S D'Alessandro,
  4. M Bauduy,
  5. P Cordes,
  6. T Harada,
  7. D I Israel,
  8. R M Hewick,
  9. K M Kerns, and
  10. P LaPan
  1. Genetics Institute, Cambridge, MA 02140.

Abstract

We have purified and characterized active recombinant human bone morphogenetic protein (BMP) 2A. Implantation of the recombinant protein in rats showed that a single BMP can induce bone formation in vivo. A dose-response and time-course study using the rat ectopic bone formation assay revealed that implantation of 0.5-115 micrograms of partially purified recombinant human BMP-2A resulted in cartilage by day 7 and bone formation by day 14. The time at which bone formation occurred was dependent on the amount of BMP-2A implanted; at high doses bone formation could be observed at 5 days. The cartilage- and bone-inductive activity of the recombinant BMP-2A is histologically indistinguishable from that of bone extracts. Thus, recombinant BMP-2A has therapeutic potential to promote de novo bone formation in humans.

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  1. PNAS March 1, 1990 vol. 87 no. 6 2220-2224
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