Inositol 1,4,5-trisphosphate receptors: distinct neuronal and nonneuronal forms derived by alternative splicing differ in phosphorylation

  1. S K Danoff,
  2. C D Ferris,
  3. C Donath,
  4. G A Fischer,
  5. S Munemitsu,
  6. A Ullrich,
  7. S H Snyder, and
  8. C A Ross
  1. Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Abstract

We have identified two distinct transcripts of inositol 1,4,5-trisphosphate receptor by using the PCR on first-strand cDNAs from various rat tissues. The longer form, corresponding to the previously cloned adult rat brain inositol 1,4,5-trisphosphate receptor, contains a 120-nucleotide insert between the two cAMP-dependent protein kinase phosphorylation consensus sequences. The shorter form (lacking the insert) predominates in fetal brain and peripheral tissues and appears to represent a nonneuronal receptor, whereas the longer form is found in adult brain and appears to be exclusively neuronal. The phosphorylation kinetics by cAMP-dependent protein kinase and the phosphopeptide maps differ for inositol 1,4,5-trisphosphate receptors purified from tissues predominantly expressing different forms of the transcript.

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  1. PNAS April 1, 1991 vol. 88 no. 7 2951-2955
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