Phosphoglucomutase 1: complete human and rabbit mRNA sequences and direct mapping of this highly polymorphic marker on human chromosome 1

  1. D B Whitehouse,
  2. W Putt,
  3. J U Lovegrove,
  4. K Morrison,
  5. M Hollyoake,
  6. M F Fox,
  7. D A Hopkinson, and
  8. Y H Edwards
  1. Medical Research Council Human Biochemical Genetics Unit, Galton Laboratory, University College London, United Kingdom.

Abstract

A cDNA clone encoding the mRNA for the highly polymorphic human enzyme phosphoglucomutase 1 (PGM1; EC 5.4.2.2) has been isolated and characterized. This was achieved indirectly by first isolating a rabbit cDNA from an expression library using anti-rabbit PGM antibodies. A comparison of the nucleotide sequences shows that the homologies between human and rabbit PGM1 mRNAs are 92% and 97% for the coding nucleotide sequence and the amino acid sequence, respectively. The derived rabbit amino acid sequence is in complete agreement with the published protein sequence for rabbit muscle PGM. A physical localization of the human PGM1 gene to chromosome 1p31 has been determined by in situ hybridization. Analysis of DNA from a wide variety of vertebrates indicates a high level of PGM1 sequence conservation during evolution.

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  1. PNAS January 1, 1992 vol. 89 no. 1 411-415
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