Dodeca satellite: a conserved G+C-rich satellite from the centromeric heterochromatin of Drosophila melanogaster

  1. J P Abad,
  2. M Carmena,
  3. S Baars,
  4. R D Saunders,
  5. D M Glover,
  6. P Ludeña,
  7. C Sentis,
  8. C Tyler-Smith, and
  9. A Villasante
  1. Centro de Biología Molecular (CSIC-UAM), Spain.

Abstract

To identify sequences from the centromeric region, we have constructed a Drosophila melanogaster yeast artificial chromosome (YAC) library and screened it with purified DNA from the minichromosome Dp(1;f)1187 derived from the X chromosome. We describe the structure of one clone isolated in this way. This YAC is structurally unstable and contains tandemly repeated G+C-rich 11-mer and 12-mer units, which we call dodeca satellite. Most of this satellite is located near the centromere of an autosome. Cross-hybridizing sequences are found in the genomes of organisms as distant as Arabidopsis thaliana and Homo sapiens.

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  1. PNAS May 15, 1992 vol. 89 no. 10 4663-4667
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