pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions

  1. M D Schaller,
  2. C A Borgman,
  3. B S Cobb,
  4. R R Vines,
  5. A B Reynolds, and
  6. J T Parsons
  1. Department of Microbiology, School of Medicine, University of Virginia, Charlottesville 22908.

Abstract

Expression of the Rous sarcoma virus-encoded oncoprotein, pp60v-src, subverts the normal regulation of cell growth, which results in oncogenic transformation. This process requires the intrinsic protein-tyrosine kinase activity of pp60v-src and is associated with an increase in tyrosine phosphorylation of a number of cellular proteins, candidate substrates for pp60v-src. We report here the isolation of a cDNA encoding a protein, pp125, that is a major phosphotyrosine-containing protein in untransformed chicken embryo cells and exhibits an increase in phosphotyrosine in pp60v-src-transformed chicken embryo cells. This cDNA encodes a cytoplasmic protein-tyrosine kinase which, based upon its predicted amino acid sequence and structure, is the prototype for an additional family of protein-tyrosine kinases. Immunofluorescence localization experiments show that pp125 is localized to focal adhesions; hence, we suggest the name focal adhesion kinase.

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  1. PNAS June 1, 1992 vol. 89 no. 11 5192-5196
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