Expression cloning of a cDNA from rabbit kidney cortex that induces a single transport system for cystine and dibasic and neutral amino acids

  1. J Bertran,
  2. A Werner,
  3. M L Moore,
  4. G Stange,
  5. D Markovich,
  6. J Biber,
  7. X Testar,
  8. A Zorzano,
  9. M Palacin, and
  10. H Murer
  1. Institute of Physiology, University of Zürich, Switzerland.

Abstract

We have isolated a cDNA clone by screening a rabbit kidney cortex cDNA library for expression of sodium-independent transport of L-arginine and L-alanine in Xenopus laevis oocytes. Expressed uptake relates to a single component of sodium-independent transport for dibasic and neutral amino acids. This transport activity resembles the functionally defined system b0,+ and carries cystine and dibasic amino acids with high affinity. The rBAT (b0,+ amino acid transporter-related) mRNA is found mainly in kidney and intestinal mucosa. It encodes a predicted 77.8-kDa protein with only one putative transmembrane domain and seven potential N-glycosylation sites. This protein could either be a constitutive element or a specific activator of system b0,+.

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  1. PNAS June 15, 1992 vol. 89 no. 12 5601-5605
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