Wild-type p53 binds to the TATA-binding protein and represses transcription

  1. E Seto,
  2. A Usheva,
  3. G P Zambetti,
  4. J Momand,
  5. N Horikoshi,
  6. R Weinmann,
  7. A J Levine, and
  8. T Shenk
  1. Howard Hughes Medical Institute, Princeton University, NJ 08544-1014.

Abstract

p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

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  1. PNAS December 15, 1992 vol. 89 no. 24 12028-12032
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