Cloning of Nrf1, an NF-E2-related transcription factor, by genetic selection in yeast

  1. J Y Chan,
  2. X L Han, and
  3. Y W Kan
  1. Department of Laboratory Medicine, University of California, San Francisco 94143-0724.

Abstract

We have devised a complementation assay in yeast to clone mammalian transcriptional activators and have used it to identify a human basic leucine-zipper transcription factor that we have designated Nrf1 for NF-E2-related factor 1. Nrf1 potentially encodes a 742-aa protein and displays marked homology to the mouse and human NF-E2 transcription factors. Nrf1 activates transcription via NF-E2 binding sites in yeast cells. The ubiquitous expression pattern of Nrf1 and the range of promoters containing the NF-E2 binding motif suggest that this gene may play a role in the regulation of heme synthesis and ferritin genes.

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  1. PNAS December 1, 1993 vol. 90 no. 23 11371-11375
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