Quantal acetylcholine release induced by mediatophore transfection

  1. J Falk-Vairant,
  2. P Corrèges,
  3. L Eder-Colli,
  4. N Salem,
  5. E Roulet,
  6. A Bloc,
  7. F Meunier,
  8. B Lesbats,
  9. F Loctin,
  10. M Synguelakis,
  11. M Israel, and
  12. Y Dunant
  1. Département Pharmacologie, C.M.U., CH-1211 Geneva, Switzerland.

Abstract

Mediatophore is a protein of approximately 200 kDa able to translocate acetylcholine in response to calcium. It was purified from the presynaptic plasma membranes of the electric organ nerve terminals. Mediatophore is a homooligomer of a 16-kDa subunit, homologous to the proteolipid of V-ATPase. Cells of the N18TG-2 neuronal line are not able to produce quantal acetylcholine release. We show here that transfection of N18TG-2 cells with a plasmid encoding the mediatophore subunit restored calcium-dependent release. The essential feature of such a release was its quantal nature, similar to what is observed in situ in cholinergic synapses from which mediatophore was purified.

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  1. PNAS May 28, 1996 vol. 93 no. 11 5203-5207
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