Effect of tissue factor deficiency on mouse and tumor development

Effect of tissue factor deficiency on mouse and tumor development

Division of Hematology, Washington University School of Medicine, Barnes–Jewish Hospital, St. Louis, MO 63110

Abstract

Previous reports suggest that tissue factor (TF) may play an essential role in embryonic vascular development and tumor angiogenesis. To further examine this relationship, the morphology of fully developed TF-deficient embryos and the growth of TF-deficient teratomas and teratocarcinomas were analyzed. In a 129/Sv genetic background, TF null embryos do not survive beyond mid-gestation. In contrast, 14% of 129/Sv × C57BL/6 TF-deficient embryos escape this early mortality and survive to birth. On gross and microscopic inspection, these late gestation, TF-deficient embryos appear normal. The growth and vascularity of TF(+/+), TF(+/−), and TF(−/−) teratomas and teratocarcinomas are indistinguishable. Thus, tumor-derived TF is not required for tumor growth and angiogenesis and the combined data do not support an essential role for TF in embryonic vascular development.

Footnotes

↵ * To whom reprint requests should be addressed.
Philip W. Majerus, Washington University School of Medicine, St. Louis, MO
ABBREVIATIONS:

TF,

tissue factor;

E,

embryonic day;

ES,

embryonic stem;

Neo,

neomycin;

Hyg,

hygromycin b;

MEF,

mouse embryonic fibroblasts;

EC,

embryonal carcinoma