Cloning of cDNA and estrogen-induced hepatic gene expression for choriogenin H, a precursor protein of the fish egg envelope (chorion)

Cloning of cDNA and estrogen-induced hepatic gene expression for choriogenin H, a precursor protein of the fish egg envelope (chorion)

^*Life Science Institute, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102, Japan; and ^†Department of Biology, School of Education, Waseda University, Nishiwaseda, Shinjukuku, Tokyo 169, Japan

Abstract

A cDNA for choriogenin H (Chg H; formerly high-molecular weight spawning female-specific substances, or H-SF), a precursor protein of the inner layer subunits of egg envelope (chorion) of the teleost fish, Oryzias latipes, was cloned and analyzed. The clone consisted of 1913 bp and contained an open reading frame encoding a signal peptide of 22 aa and Chg H protein of 569 aa. The Chg protein possessed three potential N-glycosylation sites and Pro-X-Y repeat sequences in the first two-fifths of the N terminus. There were amino acid sequence similarities between Chg H and a gene product expressed in the liver of female winter flounder during vitellogenesis. Moreover, the amino acid sequence of Chg H is similar to that of ZP2 rather than ZP3 of zona pellucida of some mammals. Northern blot analysis indicated that gene expression for Chg H occurred only in the livers of spawning female fish and 17β-estradiol-treated male fish, but not in the ovary of the spawning female fish. Gene expression for Chg H and Chg L (formerly low-molecular weight spawning female-specific substance, or L-SF) was induced and increased in parallel in the male fish liver after 17β-estradiol treatment.

Footnotes

↵ ‡ To whom reprint requests should be addressed.
Howard Bern, University of California, Berkeley, CA
Data deposition: The sequence reported in this paper has been deposited in the GenBank database (accession no. D89609D89609).
ABBREVIATIONS:

E2,

17β-estradiol;

H- and L-SF,

high- and low-molecular weight spawning female-specific substances, respectively;

Chg H and L,

choriogenin H and L, respectively;

SLIC,

single-strand ligation to single-stranded cDNA