The influence of chromosome flexibility on chromosome transport during anaphase A

Raj and Peskin. 10.1073/pnas.0601215103.

Supporting Information

Files in this Data Supplement:

Supporting Figure 3
Supporting Figure 4
Supporting Appendix
Supporting Movie 1
Supporting Movie 2
Supporting Movie 3
Supporting Movie 4

Supporting Figure 3

Fig. 3. Convergence study of computed steady-state velocity with respect to Dx. The y axis is the error, defined as the difference between the computed velocity and the optimized "theoretical" velocity of 8.742 × 10^-7 m/s. The convergence rate found is 1.76.

Supporting Figure 4

Fig. 4. Convergence study of computed steady-state velocity with respect to Ds. The y axis is the error, defined as the difference between the computed velocity and the optimized theoretical velocity of 8.777 × 10^-7 m/s. The convergence rate found is 2.61.

Supporting Movie 1

Movie 1. Simulations of chromosome motion for long (3 mm, blue), medium (1 mm, green), and short (0.5 mm red) chromosomes with physiological Young’s modulus (38 N/m²) for Df = 8 k_BT. All spatial dimensions in mm. All chromosomes here move at roughly the same speed, regardless of size.

Supporting Movie 2

Movie 2. Simulations of chromosome motion for long (3 mm, blue), medium (1 mm, green), and short (0.5 mm, red) chromosomes with a Young’s modulus of 2,423 N/m² for Df = 8 k_BT. All spatial dimensions in mm. In sharp contrast with the flexible case, the speed of the chromosome increases significantly as the size of the chromosome decreases.

Supporting Movie 3

Movie 3. Simulations of chromosome motion for long (3 mm) chromosomes with Young’s moduli of 38 and 2,423 N/m² for Df = 8 k_BT. All spatial dimensions in mm. Here, the more flexible chromosome moves significantly faster than the stiffer one.

Supporting Movie 4

Movie 4. Simulations of chromosome motion for short (0.5 mm) chromosomes with Young’s moduli of 38 and 2,423 N/m² for Df = 8 k_BT. All spatial dimensions in mm. Here, the stiffer chromosome moves faster than the more flexible one. This is the opposite of what happens for the long (3 mm) chromosomes.