Table 2

Prediction of adenylation domain specificity determining residues, amino acid substrates, and domain organization of NRPSs

PolypeptideResidue in adenylylation domain*SubstrateDomain organization
235236239278299301322330
Nrps1-1DFWNVGMVThreonineC-A-T
Nrps1-2DAWLLGAVLeucineC-A-T-E
Nrps1-3DVWHVSLLSerineA-T
DGTLTAEVTyrosineC-A-T
Nrps2-1DAQELAVLGlutamineA-T
Nrps2-2DAWLYGLVLeucineC-A-T-E
DLPKVGEVAsparagineC-A-T
Nrps2-3DVWNLSLISerineC-A-T-E
DLPKVGEVAsparagineC-A-T-E
DLPKVGEVAsparagineC-A-T-Te
Nrps3-1DMELLGLIOrnitineC-A-T
Nrps3-2NDNDNDNDNDNDNDNDE-Te
Nrps3-3DVWHVSLVSerineA-T
Nrps4DLTKLGEVAsparagineA-T
Nrps5DVQLLAHVProlineA-T
Nrps6DVQLIAHVProlineC-A-T
Nrps7-1DFETTAAVValineA-T
Nrps7-2DAKDLGVVGlutamateA
Nrps7-3DFQLLGLAPipecolateA-T
Nrps7-4DAFWLGGTValineA-T-C
Nrps7-5DAQDLGLVGlutamateA-T
Nrps7-6DFQLVGVAPipecolateC-A-T
Nrps7-7DVWHVTVVSerineA-T
Nrps7-8NDNDNDNDNDNDNDNDT-C
Nrps7-9NDNDNDNDNDNDNDNDC
Nrps7-10NDNDNDNDNDNDNDNDC
Nrps7-11DLYNLSLICysteineA-T
Nrps7-12NDNDNDNDNDNDNDNDT-C-T-Te
Nrps7-13NDNDNDNDNDNDNDNDT-C
Nrps8DLVFGLGIAlanineA
Nrps9DHESDVGICysteineA
  • * According to GrsA numbering. 

  • Undetected consensus amino acid residue. 

  • C, condensation domain; A, adenylation domain; T, thiolation domain; E, epimeration domain; Te, thioesterase domain; ND, not determined.