Skip to main content
  • Submit
  • About
    • Editorial Board
    • PNAS Staff
    • FAQ
    • Accessibility Statement
    • Rights and Permissions
    • Site Map
  • Contact
  • Journal Club
  • Subscribe
    • Subscription Rates
    • Subscriptions FAQ
    • Open Access
    • Recommend PNAS to Your Librarian
  • Log in
  • My Cart

Main menu

  • Home
  • Articles
    • Current
    • Special Feature Articles - Most Recent
    • Special Features
    • Colloquia
    • Collected Articles
    • PNAS Classics
    • List of Issues
  • Front Matter
  • News
    • For the Press
    • This Week In PNAS
    • PNAS in the News
  • Podcasts
  • Authors
    • Information for Authors
    • Editorial and Journal Policies
    • Submission Procedures
    • Fees and Licenses
  • Submit
  • About
    • Editorial Board
    • PNAS Staff
    • FAQ
    • Accessibility Statement
    • Rights and Permissions
    • Site Map
  • Contact
  • Journal Club
  • Subscribe
    • Subscription Rates
    • Subscriptions FAQ
    • Open Access
    • Recommend PNAS to Your Librarian

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Home
Home

Advanced Search

  • Home
  • Articles
    • Current
    • Special Feature Articles - Most Recent
    • Special Features
    • Colloquia
    • Collected Articles
    • PNAS Classics
    • List of Issues
  • Front Matter
  • News
    • For the Press
    • This Week In PNAS
    • PNAS in the News
  • Podcasts
  • Authors
    • Information for Authors
    • Editorial and Journal Policies
    • Submission Procedures
    • Fees and Licenses

New Research In

Physical Sciences

Featured Portals

  • Physics
  • Chemistry
  • Sustainability Science

Articles by Topic

  • Applied Mathematics
  • Applied Physical Sciences
  • Astronomy
  • Computer Sciences
  • Earth, Atmospheric, and Planetary Sciences
  • Engineering
  • Environmental Sciences
  • Mathematics
  • Statistics

Social Sciences

Featured Portals

  • Anthropology
  • Sustainability Science

Articles by Topic

  • Economic Sciences
  • Environmental Sciences
  • Political Sciences
  • Psychological and Cognitive Sciences
  • Social Sciences

Biological Sciences

Featured Portals

  • Sustainability Science

Articles by Topic

  • Agricultural Sciences
  • Anthropology
  • Applied Biological Sciences
  • Biochemistry
  • Biophysics and Computational Biology
  • Cell Biology
  • Developmental Biology
  • Ecology
  • Environmental Sciences
  • Evolution
  • Genetics
  • Immunology and Inflammation
  • Medical Sciences
  • Microbiology
  • Neuroscience
  • Pharmacology
  • Physiology
  • Plant Biology
  • Population Biology
  • Psychological and Cognitive Sciences
  • Sustainability Science
  • Systems Biology
Research Article

RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation

Krystyna Mazan-Mamczarz, Stefanie Galbán, Isabel López de Silanes, Jennifer L. Martindale, Ulus Atasoy, Jack D. Keene, and Myriam Gorospe
PNAS July 8, 2003 100 (14) 8354-8359; https://doi.org/10.1073/pnas.1432104100
Krystyna Mazan-Mamczarz
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stefanie Galbán
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Isabel López de Silanes
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jennifer L. Martindale
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ulus Atasoy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jack D. Keene
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Myriam Gorospe
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  1. Edited by Bert Vogelstein, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD (received for review April 10, 2003)

  • Article
  • Figures & SI
  • Info & Metrics
  • PDF
Loading

Abstract

Exposure to short-wavelength UV light (UVC) strongly induces p53 expression. In human RKO colorectal carcinoma cells, this increase was not due to elevated p53 mRNA abundance, cytoplasmic export of p53 mRNA, or UVC-triggered stabilization of the p53 protein. Instead, p53 translation was potently enhanced after UVC irradiation. The 3′ UTR of p53 was found to be a target of the RNA-binding protein HuR in a UVC-dependent manner in vitro and in vivo. HuR-overexpressing RKO cells displayed elevated p53 levels, whereas cells expressing reduced HuR showed markedly diminished p53 abundance and p53 translation. Our results demonstrate a role for HuR in binding to the p53 mRNA and enhancing its translation.

  • UV light
  • embryonic lethal abnormal vision

Footnotes

    • ↵‡ To whom correspondence should be addressed at: Box 12, Laboratory of Cellular and Molecular Biology, National Institute on Aging–Intramural Research Program, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224. E-mail: myriam-gorospe{at}nih.gov.

    • This paper was submitted directly (Track II) to the PNAS office.

    • Abbreviations: ELAV, embryonic lethal abnormal vision; UVC, short-wavelength UV light; REMSA, RNA electrophoretic mobility-shift assay; IP, immunoprecipitation; siRNA, small interfering RNA; CR, coding region.

    • Received April 10, 2003.
    • Accepted May 19, 2003.
    • Copyright © 2003, The National Academy of Sciences
    View Full Text
    PreviousNext
    Back to top
    Article Alerts
    Email Article

    Thank you for your interest in spreading the word on PNAS.

    NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

    Enter multiple addresses on separate lines or separate them with commas.
    RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation
    (Your Name) has sent you a message from PNAS
    (Your Name) thought you would like to see the PNAS web site.
    CAPTCHA
    This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
    Citation Tools
    RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation
    Krystyna Mazan-Mamczarz, Stefanie Galbán, Isabel López de Silanes, Jennifer L. Martindale, Ulus Atasoy, Jack D. Keene, Myriam Gorospe
    Proceedings of the National Academy of Sciences Jul 2003, 100 (14) 8354-8359; DOI: 10.1073/pnas.1432104100

    Citation Manager Formats

    • BibTeX
    • Bookends
    • EasyBib
    • EndNote (tagged)
    • EndNote 8 (xml)
    • Medlars
    • Mendeley
    • Papers
    • RefWorks Tagged
    • Ref Manager
    • RIS
    • Zotero
    Request Permissions
    Share
    RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation
    Krystyna Mazan-Mamczarz, Stefanie Galbán, Isabel López de Silanes, Jennifer L. Martindale, Ulus Atasoy, Jack D. Keene, Myriam Gorospe
    Proceedings of the National Academy of Sciences Jul 2003, 100 (14) 8354-8359; DOI: 10.1073/pnas.1432104100
    Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
    • Tweet Widget
    • Facebook Like
    • Mendeley logo Mendeley
    Proceedings of the National Academy of Sciences: 100 (14)
    Table of Contents

    Submit

    Sign up for Article Alerts

    Jump to section

    • Article
      • Abstract
      • Materials and Methods
      • Results and Discussion
      • Acknowledgments
      • Footnotes
      • References
    • Figures & SI
    • Info & Metrics
    • PDF

    You May Also be Interested in

    Abstract depiction of a guitar and musical note
    Science & Culture: At the nexus of music and medicine, some see disease treatments
    Although the evidence is still limited, a growing body of research suggests music may have beneficial effects for diseases such as Parkinson’s.
    Image credit: Shutterstock/agsandrew.
    Large piece of gold
    News Feature: Tracing gold's cosmic origins
    Astronomers thought they’d finally figured out where gold and other heavy elements in the universe came from. In light of recent results, they’re not so sure.
    Image credit: Science Source/Tom McHugh.
    Dancers in red dresses
    Journal Club: Friends appear to share patterns of brain activity
    Researchers are still trying to understand what causes this strong correlation between neural and social networks.
    Image credit: Shutterstock/Yeongsik Im.
    White and blue bird
    Hazards of ozone pollution to birds
    Amanda Rodewald, Ivan Rudik, and Catherine Kling talk about the hazards of ozone pollution to birds.
    Listen
    Past PodcastsSubscribe
    Goats standing in a pin
    Transplantation of sperm-producing stem cells
    CRISPR-Cas9 gene editing can improve the effectiveness of spermatogonial stem cell transplantation in mice and livestock, a study finds.
    Image credit: Jon M. Oatley.

    Similar Articles

    Site Logo
    Powered by HighWire
    • Submit Manuscript
    • Twitter
    • Facebook
    • RSS Feeds
    • Email Alerts

    Articles

    • Current Issue
    • Special Feature Articles – Most Recent
    • List of Issues

    PNAS Portals

    • Anthropology
    • Chemistry
    • Classics
    • Front Matter
    • Physics
    • Sustainability Science
    • Teaching Resources

    Information

    • Authors
    • Editorial Board
    • Reviewers
    • Librarians
    • Press
    • Site Map
    • PNAS Updates

    Feedback    Privacy/Legal

    Copyright © 2021 National Academy of Sciences. Online ISSN 1091-6490