GENETICS. For the article “A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes, ” by Susanne M. D. Goldberg, Justin Johnson, Dana Busam, Tamara Feldblyum, Steve Ferriera, Robert Friedman, Aaron Halpern, Hoda Khouri, Saul A. Kravitz, Federico M. Lauro, Kelvin Li, Yu-Hui Rogers, Robert Strausberg, Granger Sutton, Luke Tallon, Torsten Thomas, Eli Venter, Marvin Frazier, and J. Craig Venter, which appeared in issue 30, July 25, 2006, of Proc Natl Acad Sci USA (103:11240–11245; first published July 13, 2006; 10.1073/pnas.0604351103), the authors note that in Fig. 3, the text in the lower green box, “Reference Genome Unavailable,” should read “Reference Genome Available.” The corrected figure and its legend appear below. This error does not effect the conclusions of the article.
Decision tree for hybrid sequencing strategy. For organisms with a small genome size (<3 Mb) and/or a small number of gaps and/or high levels of repetitive structure inducing physical ends, we found 8× Sanger sequencing to be the most cost-effective approach. For organisms with a large genome size, many sequencing gaps, and/or hard stops, we found initial sequencing of 5.3× Sanger data followed by the addition of two 454 runs to be the most cost-effective approach.
