Several aspects of the proposal, which aims to expand open access, require serious discussion and, in some cases, a rethink.
Image credit: Dave Cutler (artist).
New Research In
Physical Sciences
Featured Portals
Articles by Topic
Biological Sciences
Featured Portals
Articles by Topic
-
Edited by Jacqueline K. Barton, California Institute of Technology, Pasadena, CA, and approved November 17, 2006 (received for review August 24, 2006)
Article Figures & SI
Figures
- Fig. 1.
Experimental outline. (A) Structures of adenine (A), EA, and εA. (B) Overview of experimental procedure for mutation detection.
- Fig. 2.
Lesion bypass of EA and εA in wild-type and alkB E. coli as compared with a lesion-free control containing thymine (T) at the relevant site.
- Fig. 3.
MALDI-TOF mass spectra of in vitro repair reaction of AlkB with a 16-mer oligonucleotide containing a single EA residue. (A) Control incubation of oligonucleotide and molecular weight standards without AlkB. (B) Complete conversion of EA to +16 and +135 products after a 30-min incubation at 37°C. (C) Complete conversion to a single +211 product by the addition of a 100-fold excess of PFBHA after incubation with AlkB.
- Fig. 4.
Putative structures of repair reaction products as seen by mass spectrometry. (A) Hydroxylated product (EA + 16) of AlkB metabolism of EA and its ring-opened aldehydic isomer. (B) Product (EA + 135) of ring-opened isomer reaction with anthranilic acid. (C) Product (EA + 211) of ring-opened isomer reaction with PFBHA.
- Fig. 5.
Mutational frequency and specificity of EA and εA replicated in wild-type and alkB E. coli.
- Fig. 6.
Proposed reaction pathway through which AlkB metabolizes EA. Restoration of hydrogen bonding ability might favor formation of the reactive ring-opened aldehydic species in double-stranded DNA.
Alleviation of 1,N6-ethanoadenine genotoxicity by the Escherichia coli adaptive response protein AlkB
Sign up for Article Alerts
Jump to section
You May Also be Interested in
Several large or long-lived animals seem strangely resistant to developing cancer. Elucidating the reasons why could lead to promising cancer-fighting strategies in humans.
Image credit: Shutterstock.com/ronnybas frimages.
PNAS Profile of NAS member and biochemist Hao Wu
A study suggests a putative gene-expression hallmark common to monogamous male vertebrates of some species, namely cichlid fishes, dendrobatid frogs, passeroid songbirds, common voles, and deer mice, and identifies 24 candidate genes potentially associated with monogamy.
Image courtesy of Yusan Yang (University of Pittsburgh, Pittsburgh).
Physical and social well-being in old age are linked to self-assessments of life worth, and a spectrum of behavioral, economic, health, and social variables may influence whether aging individuals believe they are leading meaningful lives.
Image courtesy of Pixabay/MabelAmber.
More Articles of This Classification
Biological Sciences
Related Content
Cited by...
- 1,N6-{alpha}-hydroxypropanoadenine, the acrolein adduct to adenine, is a substrate for AlkB dioxygenase
- Comprehensive Assessment of Oxidatively Induced Modifications of DNA in a Rat Model of Human Wilson's Disease
- The AlkB Family of Fe(II)/{alpha}-Ketoglutarate-dependent Dioxygenases: Repairing Nucleic Acid Alkylation Damage and Beyond
- AlkB Dioxygenase Preferentially Repairs Protonated Substrates: SPECIFICITY AGAINST EXOCYCLIC ADDUCTS AND MOLECULAR MECHANISM OF ACTION
- DNA Repair by Reversal of DNA Damage
- The Aminoglycoside Antibiotic Kanamycin Damages DNA Bases in Escherichia coli: Caffeine Potentiates the DNA-Damaging Effects of Kanamycin while Suppressing Cell Killing by Ciprofloxacin in Escherichia coli and Bacillus anthracis
- Spectroscopic and magnetic studies of wild-type and mutant forms of the Fe(II)- and 2-oxoglutarate-dependent decarboxylase ALKBH4
- Enzymological and structural studies of the mechanism of promiscuous substrate recognition by the oxidative DNA repair enzyme AlkB