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Research Article

Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart

Takabumi Inagawa, Tomoko Yamada-Inagawa, Trevor Eydmann, I. Saira Mian, Teresa S. Wang, and Jacob Z. Dalgaard
PNAS May 12, 2009 106 (19) 7927-7932; https://doi.org/10.1073/pnas.0812323106
Takabumi Inagawa
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Tomoko Yamada-Inagawa
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Trevor Eydmann
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I. Saira Mian
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Teresa S. Wang
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Jacob Z. Dalgaard
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  • For correspondence: j.dalgaard@mcri.ac.uk
  1. Edited by Marlene Belfort, New York State Department of Health, Albany, NY, and approved March 16, 2009

  2. ↵1T.Y. and T.Y.-I. contributed equally to this work. (received for review December 7, 2008)

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Abstract

Here, we identify a phylogenetically conserved Schizosaccharomyces pombe factor, named Rtf2, as a key requirement for efficient replication termination at the site-specific replication barrier RTS1. We show that Rtf2, a proliferating cell nuclear antigen-interacting protein, promotes termination at RTS1 by preventing replication restart; in the absence of Rtf2, we observe the establishment of “slow-moving” Srs2-dependent replication forks. Analysis of the pmt3 (SUMO) and rtf2 mutants establishes that pmt3 causes a reduction in RTS1 barrier activity, that rtf2 and pmt3 are nonadditive, and that pmt3 (SUMO) partly suppresses the rtf2-dependent replication restart. Our results are consistent with a model in which Rtf2 stabilizes the replication fork stalled at RTS1 until completion of DNA synthesis by a converging replication fork initiated at a flanking origin.

  • proliferating cell nuclear antigen
  • RTS1
  • SUMO
  • Srs2
  • Rtf1

Footnotes

  • 2To whom correspondence should be addressed. E-mail: j.dalgaard{at}mcri.ac.uk
  • Author contributions: T.I., T.Y.-I., I.S.M., T.S.W., and J.Z.D. designed research; T.I., T.Y.-I., T.E., I.S.M., and J.Z.D. performed research; T.I., T.Y.-I., I.S.M., and J.Z.D. analyzed data; and T.I., T.Y.-I., and J.Z.D. wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

  • Data deposition: The sequence reported in this paper has been deposited in the GenBank database (accession no. FN376423).

  • This article contains supporting information online at www.pnas.org/cgi/content/full/0812323106/DCSupplemental.

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Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart
Takabumi Inagawa, Tomoko Yamada-Inagawa, Trevor Eydmann, I. Saira Mian, Teresa S. Wang, Jacob Z. Dalgaard
Proceedings of the National Academy of Sciences May 2009, 106 (19) 7927-7932; DOI: 10.1073/pnas.0812323106

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Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart
Takabumi Inagawa, Tomoko Yamada-Inagawa, Trevor Eydmann, I. Saira Mian, Teresa S. Wang, Jacob Z. Dalgaard
Proceedings of the National Academy of Sciences May 2009, 106 (19) 7927-7932; DOI: 10.1073/pnas.0812323106
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