Two-photon excitation of channelrhodopsin-2 at saturation

^aDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544;
^bThe Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544;
^cPrinceton Neuroscience Institute, Carl Icahn Laboratory, Princeton University, Princeton, NJ 08544; and
^dDepartment of Physics, Princeton University, Princeton, NJ 08544

Contributed by David W. Tank, June 25, 2009 (received for review May 12, 2009)

Footnotes

¹To whom correspondence should be addressed. E-mail: dwtank{at}princeton.edu
Author contributions: J.P.R. and D.W.T. designed research; J.P.R. and D.W.T. performed research; J.P.R. analyzed data; and J.P.R. and D.W.T. wrote the paper.
The authors declare no conflict of interest.
This article contains supporting information online at www.pnas.org/cgi/content/full/0907084106/DCSupplemental.

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	Abstract	Full	Pdf
Sep 2009	2587	271	472
Oct 2009	277	155	170
Nov 2009	147	133	87
Dec 2009	89	53	49
Total 2009	3100	612	778
Jan 2010	74	56	50
Feb 2010	67	41	33
Mar 2010	59	80	45
Apr 2010	46	70	46
May 2010	48	53	31
Jun 2010	48	55	27
Jul 2010	36	46	25
Aug 2010	43	41	29
Sep 2010	45	53	39
Oct 2010	65	53	40
Nov 2010	53	57	34
Dec 2010	35	25	24
Total 2010	619	630	423
Jan 2011	40	34	30
Feb 2011	25	39	24
Mar 2011	25	59	30
Apr 2011	40	36	36
May 2011	33	26	23
Jun 2011	36	46	47
Jul 2011	44	38	52
Aug 2011	38	28	53
Sep 2011	37	33	28
Oct 2011	45	56	56
Nov 2011	40	49	36
Dec 2011	36	33	27
Total 2011	439	477	442
Jan 2012	33	42	46
Feb 2012	59	43	46
Mar 2012	74	52	34
Apr 2012	42	32	34
May 2012	43	51	42
Jun 2012	33	34	28
Jul 2012	54	49	32
Aug 2012	57	25	25
Sep 2012	33	41	34
Oct 2012	56	41	25
Nov 2012	61	42	27
Dec 2012	32	26	25
Total 2012	577	478	398
Jan 2013	78	42	36
Feb 2013	51	27	33
Mar 2013	51	25	45
Apr 2013	42	32	37
May 2013	34	47	36
Jun 2013	41	49	23
Jul 2013	43	67	41
Aug 2013	31	18	21
Sep 2013	52	26	23
Oct 2013	48	35	36
Nov 2013	58	24	35
Dec 2013	42	40	66
Total 2013	571	432	432
Jan 2014	39	30	31
Feb 2014	21	27	23
Mar 2014	53	29	35
Apr 2014	38	44	49
May 2014	30	32	24
Jun 2014	45	39	27
Jul 2014	39	28	37
Aug 2014	46	33	30
Sep 2014	51	44	41
Oct 2014	48	74	69
Nov 2014	6595	32	20
Dec 2014	2537	32	21
Total 2014	9542	444	407
Jan 2015	1918	41	43
Feb 2015	2002	69	36
Mar 2015	1250	35	21
Apr 2015	1053	43	25
May 2015	756	79	37
Jun 2015	1763	54	41
Jul 2015	3749	55	40
Aug 2015	3968	68	29
Sep 2015	3723	44	28
Oct 2015	4465	43	28
Nov 2015	3894	39	34
Dec 2015	3733	25	38
Total 2015	32274	595	400
Jan 2016	3472	31	33
Feb 2016	3289	38	39
Mar 2016	3146	43	20
Apr 2016	3385	65	28
May 2016	3571	61	26
Jun 2016	3584	87	24
Jul 2016	3576	45	24
Aug 2016	3678	65	38
Sep 2016	3029	46	53
Oct 2016	3043	81	24
Nov 2016	2916	40	31
Dec 2016	3193	28	16
Total 2016	39882	630	356
Jan 2017	2648	24	13
Feb 2017	2647	36	12
Mar 2017	2674	28	17
Apr 2017	2748	16	12
May 2017	1674	23	10
Jun 2017	4219	33	13
Jul 2017	6612	26	16
Aug 2017	3229	28	9
Sep 2017	3551	29	33
Oct 2017	3593	37	20
Nov 2017	3008	37	12
Dec 2017	47	33	27
Total 2017	36650	350	194
Jan 2018	41	37	21
Feb 2018	61	56	21
Mar 2018	44	49	24
Apr 2018	44	53	25
May 2018	43	51	33
Jun 2018	38	46	17
Jul 2018	46	45	22
Aug 2018	60	52	25
Sep 2018	32	32	26
Oct 2018	13	47	24
Nov 2018	9	57	31
Dec 2018	7	39	21
Total 2018	438	564	290
Jan 2019	6	60	26
Feb 2019	5	54	23
Mar 2019	6	34	18
Apr 2019	3	75	17
May 2019	8	74	29
Jun 2019	6	34	18
Jul 2019	19	57	35
Aug 2019	3	35	37
Sep 2019	4	61	28
Oct 2019	12	80	40
Nov 2019	5	58	13
Dec 2019	16	57	10
Total 2019	93	679	294
Jan 2020	18	75	30
Feb 2020	3	86	18
Mar 2020	8	56	22
Apr 2020	6	51	28
May 2020	7	73	26
Jun 2020	9	79	34
Jul 2020	6	67	12
Aug 2020	6	89	23
Sep 2020	7	84	39
Oct 2020	6	74	31
Nov 2020	15	76	34
Dec 2020	6	61	22
Total 2020	97	871	319
Jan 2021	14	91	31
Feb 2021	4	72	26
Mar 2021	7	77	16
Apr 2021	6	19	4
Total 2021	31	259	77
Total	124313	7021	4810