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Research Article

Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns

Maria G. Dominguez-Bello, Elizabeth K. Costello, Monica Contreras, Magda Magris, Glida Hidalgo, Noah Fierer, and Rob Knight
  1. aDepartment of Biology, University of Puerto Rico, San Juan, Puerto Rico 00931;
  2. bDepartment of Chemistry and Biochemistry,
  3. eDepartment of Ecology and Evolutionary Biology, and
  4. fCooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, CO 80305;
  5. cCenter of Biophysics and Biochemistry, Venezuelan Institute for Scientific Research, Caracas 1020A, Venezuela;
  6. dAmazonic Center for Research and Control of Tropical Diseases, Puerto Ayacucho 7101, Amazonas, Venezuela; and
  7. gThe Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80305

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PNAS June 29, 2010 107 (26) 11971-11975; https://doi.org/10.1073/pnas.1002601107
Maria G. Dominguez-Bello
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  • For correspondence: maria.dominguez1@upr.edu
Elizabeth K. Costello
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Monica Contreras
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Magda Magris
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Glida Hidalgo
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Noah Fierer
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Rob Knight
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  1. Edited by Jeffrey I. Gordon, Washington University School of Medicine, St. Louis, MO, and approved May 24, 2010 (received for review March 2, 2010)

  2. ↵1M.G.D.-B. and E.K.C. contributed equally to this work.

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Abstract

Upon delivery, the neonate is exposed for the first time to a wide array of microbes from a variety of sources, including maternal bacteria. Although prior studies have suggested that delivery mode shapes the microbiota's establishment and, subsequently, its role in child health, most researchers have focused on specific bacterial taxa or on a single body habitat, the gut. Thus, the initiation stage of human microbiome development remains obscure. The goal of the present study was to obtain a community-wide perspective on the influence of delivery mode and body habitat on the neonate's first microbiota. We used multiplexed 16S rRNA gene pyrosequencing to characterize bacterial communities from mothers and their newborn babies, four born vaginally and six born via Cesarean section. Mothers’ skin, oral mucosa, and vagina were sampled 1 h before delivery, and neonates’ skin, oral mucosa, and nasopharyngeal aspirate were sampled <5 min, and meconium <24 h, after delivery. We found that in direct contrast to the highly differentiated communities of their mothers, neonates harbored bacterial communities that were undifferentiated across multiple body habitats, regardless of delivery mode. Our results also show that vaginally delivered infants acquired bacterial communities resembling their own mother's vaginal microbiota, dominated by Lactobacillus, Prevotella, or Sneathia spp., and C-section infants harbored bacterial communities similar to those found on the skin surface, dominated by Staphylococcus, Corynebacterium, and Propionibacterium spp. These findings establish an important baseline for studies tracking the human microbiome's successional development in different body habitats following different delivery modes, and their associated effects on infant health.

  • host–microbe interactions
  • human microbiome
  • neonatal bacterial assemblages
  • pioneer community

Footnotes

  • 2To whom correspondence should be addressed. E-mail: maria.dominguez1{at}upr.edu.
  • Author contributions: M.G.D.-B. designed research; M.G.D.-B., E.K.C., M.C., M.M., G.H., N.F., and R.K. performed research; M.G.D.-B., E.K.C., N.F., and R.K. analyzed data; and M.G.D.-B., E.K.C., and R.K. wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

  • Database deposition: The sequence reported in this paper has been deposited in the Sequence Read Archive database (ID 020060).

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1002601107/-/DCSupplemental.

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    Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns
    Maria G. Dominguez-Bello, Elizabeth K. Costello, Monica Contreras, Magda Magris, Glida Hidalgo, Noah Fierer, Rob Knight
    Proceedings of the National Academy of Sciences Jun 2010, 107 (26) 11971-11975; DOI: 10.1073/pnas.1002601107

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    Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns
    Maria G. Dominguez-Bello, Elizabeth K. Costello, Monica Contreras, Magda Magris, Glida Hidalgo, Noah Fierer, Rob Knight
    Proceedings of the National Academy of Sciences Jun 2010, 107 (26) 11971-11975; DOI: 10.1073/pnas.1002601107
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