Although the evidence is still limited, a growing body of research suggests music may have beneficial effects for diseases such as Parkinson’s.
Image credit: Shutterstock/agsandrew.
New Research In
Physical Sciences
Featured Portals
Articles by Topic
Biological Sciences
Featured Portals
Articles by Topic
Edited* by Susan Gottesman, National Cancer Institute, Bethesda, MD, and approved July 20, 2010 (received for review June 3, 2010)
Abstract
Siderophores are essential factors for iron (Fe) acquisition in bacteria during colonization and infection of eukaryotic hosts, which restrain iron access through iron-binding protein, such as lactoferrin and transferrin. The synthesis of siderophores by Escherichia coli is considered to be fully regulated at the transcriptional level by the Fe-responsive transcriptional repressor Fur. Here we characterized two different pathways that promote the production of the siderophore enterobactin via the action of the small RNA RyhB. First, RyhB is required for normal expression of an important enterobactin biosynthesis polycistron, entCEBAH. Second, RyhB directly represses the translation of cysE, which encodes a serine acetyltransferase that uses serine as a substrate for cysteine biosynthesis. Reduction of CysE activity by RyhB allows serine to be used as building blocks for enterobactin synthesis through the nonribosomal peptide synthesis pathway. Thus, RyhB plays an essential role in siderophore production and may modulate bacterial virulence through optimization of siderophore production.
Footnotes
- 1To whom correspondence should be addressed. E-mail: eric.masse{at}usherbrooke.ca.
Author contributions: H.S., J.M.S., M.C., M.E.S.M., C.M.D., J.I., and E.M. designed research; H.S., P.L.-B., J.M.S., M.C., J.-A.M.B., M.E.S.M., F.L., C.M.D., and J.I. performed research; H.S., P.L.-B., J.M.S., M.C., J.-A.M.B., M.E.S.M., F.L., C.M.D., and J.I. contributed new reagents/analytic tools; H.S., P.L.-B., J.M.S., M.C., J.-A.M.B., M.E.S.M., F.L., C.M.D., J.I., and E.M. analyzed data; and H.S., C.M.D., J.I., and E.M. wrote the paper.
The authors declare no conflict of interest.
↵*This Direct Submission article had a prearranged editor.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1007805107/-/DCSupplemental.
A small RNA promotes siderophore production through transcriptional and metabolic remodeling
Sign up for Article Alerts
Article Classifications
- Biological Sciences
- Microbiology
Jump to section
You May Also be Interested in
Astronomers thought they’d finally figured out where gold and other heavy elements in the universe came from. In light of recent results, they’re not so sure.
Image credit: Science Source/Tom McHugh.
Researchers are still trying to understand what causes this strong correlation between neural and social networks.
Image credit: Shutterstock/Yeongsik Im.
Amanda Rodewald, Ivan Rudik, and Catherine Kling talk about the hazards of ozone pollution to birds.
CRISPR-Cas9 gene editing can improve the effectiveness of spermatogonial stem cell transplantation in mice and livestock, a study finds.
Image credit: Jon M. Oatley.