Better explicating the strengths and shortcomings of these models will help refine projections and improve transparency in the years ahead.
Image credit: Witsawat.S.
Edited by Omid C. Farokhzad, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, and accepted by the Editorial Board May 24, 2011 (received for review April 26, 2011)
Abstract
Efforts to extend nanoparticle residence time in vivo have inspired many strategies in particle surface modifications to bypass macrophage uptake and systemic clearance. Here we report a top-down biomimetic approach in particle functionalization by coating biodegradable polymeric nanoparticles with natural erythrocyte membranes, including both membrane lipids and associated membrane proteins for long-circulating cargo delivery. The structure, size and surface zeta potential, and protein contents of the erythrocyte membrane-coated nanoparticles were verified using transmission electron microscopy, dynamic light scattering, and gel electrophoresis, respectively. Mice injections with fluorophore-loaded nanoparticles revealed superior circulation half-life by the erythrocyte-mimicking nanoparticles as compared to control particles coated with the state-of-the-art synthetic stealth materials. Biodistribution study revealed significant particle retention in the blood 72 h following the particle injection. The translocation of natural cellular membranes, their associated proteins, and the corresponding functionalities to the surface of synthetic particles represents a unique approach in nanoparticle functionalization.
Footnotes
- ↵1To whom correspondence should be addressed. E-mail: zhang{at}ucsd.edu.
Author contributions: C.-M.J.H., Li Zhang, and Liangfang Zhang designed research; C.-M.J.H., Li Zhang, S.A., C.C., and R.F. performed research; C.-M.J.H., Li Zhang, S.A., C.C., R.F., and Liangfang Zhang analyzed data; and C.-M.J.H. and Liangfang Zhang wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission. O.C.F. is a guest editor invited by the Editorial Board.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1106634108/-/DCSupplemental.
Erythrocyte membrane-camouflaged polymeric nanoparticles as a biomimetic delivery platform
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