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Research Article

Protection from UV-induced skin carcinogenesis by genetic inhibition of the ataxia telangiectasia and Rad3-related (ATR) kinase

Masaoki Kawasumi, Bianca Lemos, James E. Bradner, Renee Thibodeau, Yong-son Kim, Miranda Schmidt, Erin Higgins, Sang-wahn Koo, Aimee Angle-Zahn, Adam Chen, Douglas Levine, Lynh Nguyen, Timothy P. Heffernan, Isabel Longo, Anna Mandinova, Yao-Ping Lu, Allan H. Conney, and Paul Nghiem
  1. aDivision of Dermatology, Department of Medicine, University of Washington, Seattle, WA 98109;
  2. bCutaneous Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129;
  3. cDana-Farber Cancer Institute, Boston, MA 02115;
  4. dSusan Lehman Cullman Laboratory for Cancer Research, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854; and
  5. eClinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109

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PNAS August 16, 2011 108 (33) 13716-13721; https://doi.org/10.1073/pnas.1111378108
Masaoki Kawasumi
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Bianca Lemos
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James E. Bradner
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Renee Thibodeau
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Yong-son Kim
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Miranda Schmidt
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Erin Higgins
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Sang-wahn Koo
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Aimee Angle-Zahn
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Adam Chen
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Douglas Levine
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Lynh Nguyen
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Timothy P. Heffernan
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Isabel Longo
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Anna Mandinova
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Yao-Ping Lu
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Allan H. Conney
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  • For correspondence: aconney@pharmacy.rutgers.edu pnghiem@uw.edu
Paul Nghiem
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  • For correspondence: aconney@pharmacy.rutgers.edu pnghiem@uw.edu
  1. Contributed by Allan H. Conney, July 14, 2011 (sent for review April 26, 2011)

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Abstract

Multiple human epidemiologic studies link caffeinated (but not decaffeinated) beverage intake with significant decreases in several types of cancer, including highly prevalent UV-associated skin carcinomas. The mechanism by which caffeine protects against skin cancer is unknown. Ataxia telangiectasia and Rad3-related (ATR) is a replication checkpoint kinase activated by DNA stresses and is one of several targets of caffeine. Suppression of ATR, or its downstream target checkpoint kinase 1 (Chk1), selectively sensitizes DNA-damaged and malignant cells to apoptosis. Agents that target this pathway are currently in clinical trials. Conversely, inhibition of other DNA damage response pathways, such as ataxia telangiectasia mutated (ATM) and BRCA1, promotes cancer. To determine the effect of replication checkpoint inhibition on carcinogenesis, we generated transgenic mice with diminished ATR function in skin and crossed them into a UV-sensitive background, Xpc−/−. Unlike caffeine, this genetic approach was selective and had no effect on ATM activation. These transgenic mice were viable and showed no histological abnormalities in skin. Primary keratinocytes from these mice had diminished UV-induced Chk1 phosphorylation and twofold augmentation of apoptosis after UV exposure (P = 0.006). With chronic UV treatment, transgenic mice remained tumor-free for significantly longer (P = 0.003) and had 69% fewer tumors at the end of observation of the full cohort (P = 0.019), compared with littermate controls with the same genetic background. This study suggests that inhibition of replication checkpoint function can suppress skin carcinogenesis and supports ATR inhibition as the relevant mechanism for the protective effect of caffeinated beverage intake in human epidemiologic studies.

  • skin cancer prevention
  • squamous cell carcinoma
  • xeroderma pigmentosum

Multiple human epidemiologic studies have revealed that coffee or tea intake decreases the risk of UV-associated nonmelanoma skin cancers (1–4) and non-UV–associated cancers, most prominently hepatocellular and endometrial cancers (5). In the largest study, among 93,676 women, each daily cup of caffeinated coffee consumption was dose-dependently associated with a 5% reduction in the prevalence of nonmelanoma skin cancers, whereas decaffeinated coffee had no effect, and tea had an intermediate effect, consistent with its caffeine content (3). These observations in humans hold true in multiple related studies in mice. Oral intake of caffeine in the drinking water of chronically irradiated SKH-1 hairless mice suppressed UV-induced skin cancer development (6). Topical application of caffeine in UV-pretreated “high-risk” mice also inhibited UV-induced squamous cell carcinomas (SCCs) in hairless mice by 72% (7).

UV-induced skin tumor development is a complex, long-term pathological process in vivo, and it is unclear how caffeine suppresses UV-induced tumorigenesis. Regulation of apoptosis could be one relevant factor in UV carcinogenesis, given the fact that transgenic mice expressing the apoptosis inhibitor Survivin showed accelerated development of UV-induced SCCs (8). Topical application of caffeine immediately after UV irradiation of SKH-1 hairless mice enhanced UV-induced apoptotic sunburn cells in skin (9, 10). Thus, caffeine's ability to augment elimination of UV-damaged cells via apoptosis may be relevant for its protective effects on UV-induced skin tumor development.

Caffeine is an inhibitor of several cellular processes, including activation of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) (11), both of which are protein kinases that sense various types of DNA damage to block cell-cycle progression and facilitate DNA repair (12, 13). Furthermore, caffeine has other molecular targets, including cAMP phosphodiesterase, mammalian target of rapamycin (mTOR), phosphoinositide 3-kinases, and adenosine receptors (11, 14, 15). Thus, it is important to identify the molecular targets responsible for the preventive effect of caffeine in UV carcinogenesis.

Inhibition of ATR after genotoxic stress leads to premature chromatin condensation and p53-independent apoptosis (16). Thus, there is considerable interest in the pharmacologic community in targeting the replication checkpoint via ATR or its downstream target checkpoint kinase 1 (Chk1) to sensitize cancers to DNA-damaging agents (13, 17). In this study, we hypothesized that inhibition of ATR function in vivo could lead to suppression of UV-induced tumorigenesis as a potential basis for the epidemiologically significant skin cancer-preventive effects of caffeine. Conversely, there is concern that inhibition of DNA damage response pathways may lead to genomic instability and promote cancer, as is well documented for loss of ATM and BRCA1 function (18, 19). Because of these two opposing effects of DNA damage checkpoint inhibition, determination of the net effect of ATR inhibition on cancer development is best performed by in vivo experimentation.

An appealing approach to determine the net effect of suppression of ATR function is to genetically inhibit ATR in mouse skin and study susceptibility to UV carcinogenesis. The choice of an appropriate genetic system was complicated by the fact that homozygous ATR knockout mice (Atr−/−) were embryonic lethal (20). Heterozygous Atr+/− mice demonstrated a small decrease in survival and an increase in spontaneous development of tumors, including lymphoma and hepatocellular adenoma late in life (20). Mice hypomorphic for ATR (in which ATR expression was almost undetectable because of incorporation of the Seckel mutation and aberrant splicing) demonstrated no spontaneous tumor development but showed accelerated aging and diminished lifespan (21). These studies suggest that preserving significant ATR function is important for viability but that partial or tissue-specific inhibition of ATR may be well tolerated.

Here we report the generation of transgenic mice with low-level expression of a kinase-inactive form of ATR. Keratinocytes from these mice had partial inhibition of replication checkpoint function, and the mice were protected from UV-induced skin carcinogenesis. These data further support the notion that ATR inhibition is the likely mechanism for the cancer-preventive effects of caffeine.

Results

Generation of Transgenic Mice Expressing a Kinase-Inactive Form of ATR in Skin.

We generated transgenic mice expressing a kinase-dead form of human ATR (ATR-kd) under the human keratin-14 (K14) promoter (Fig. 1A) to target expression to the basal layer of the epidermis (22). The presence of the β-globin intron (associated with improved mRNA stability) (23) was required for expression of ATR-kd under the K14 promoter, perhaps because of the large size (>8 kb) of ATR. Compared with wild-type ATR, the kinase-inactive (ATR-kd) protein has a single amino-acid substitution of Asp2475 to Ala in the conserved kinase domain and thus lacks kinase activity (24). ATR-kd acts as a dominant negative: previous studies showed that the phenotype of ATR-kd expression was essentially the same as that resulting from suppression of wild-type ATR function. Specifically, ATR-kd expression (24, 25), decreased ATR expression attributable to Seckel mutation (26, 27), and ATR knockdown by siRNA (28) all showed hypersensitivity to UV and decreased phosphorylation of Chk1.

Fig. 1.
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Fig. 1.

Generation of K14 promoter-driven ATR-kd transgenic mice. (A) Schematic of ATR-kd transgene. FLAG-tagged, human ATR-kd was inserted into the polylinker (PL) region of the human K14 promoter/enhancer (P/E) construct containing a β-globin intron. Asterisk indicates the mutation site for inactivation of ATR kinase activity (Asp2475 → Ala2475). Solid arrows indicate the primer set used to detect the transgene in genomic DNA (B). Dashed arrows indicate the primer set used to quantify mRNA expression of the transgene (C). (B) ATR-kd transgene (ATR-kd Tg) is detected in genomic DNA from transgenic (Tg) mice, not from littermate controls (Ctrl), via PCR amplification and agarose gel analysis. (C) ATR-kd transgene mRNA is expressed in transgenic (Tg) mice, but not in transgene-negative littermate controls (Ctrl), as revealed by real-time RT-PCR analysis. (Left) Amplification plot of ATR-kd transgene. cDNA containing ATR-kd transgene sequence was used as positive control (cDNA). Samples from littermate controls (Ctrl) and negative control samples (no template) showed no amplification after 40 cycles. (Right) Amplification plot of the RNA quality control gene Gapdh. (D) FLAG-tagged ATR-kd protein is expressed in ATR-kd transgenic (Tg) mouse keratinocytes. Lysates (500 μg of total protein) of primary keratinocytes isolated from Tg mice and transgene-negative littermate controls (Ctrl) were used for immunoprecipitation (IP) with anti-FLAG antibody, followed by immunoblot (IB) analyses with the indicated antibodies. Input samples (15 μg of total protein) were from the same cell lysates but were not immunoprecipitated. The anti-ATR antibody used detects both human and mouse proteins.

Because the FVB mouse strain in which these transgenic mice were created was highly resistant to UV carcinogenesis, we crossed the ATR-kd transgenic mice into an Xpc−/− background. Xpc−/− mice have a global genome-repair deficiency, are sensitive to UV, and are prone to develop tumors after a relatively short period of UV treatment, recapitulating the human disease xeroderma pigmentosum complementation group C (XPC) (29). All ATR-kd transgenic mice and transgene-negative littermate controls used in this study were Xpc−/−. After generating these transgenic mice, we extensively searched for phenotypic differences between ATR-kd transgenic mice and transgene-negative littermate controls. No obvious differences were noted in weight gain, macroscopic appearance of skin, density of hair, wound healing, or skin structures by histological examination. ATR-kd transgenic mice showed no spontaneous skin tumor development in either the FVB or Xpc−/− background.

We first confirmed stable genomic transgene integration into the transgenic mice via detection of ATR-kd DNA by using PCR (Fig. 1B). Next, we examined the expression of ATR-kd transgene at the RNA and protein levels. Expression of transgene mRNA was quantitated by real-time RT-PCR. Transgene mRNA was detected only in ATR-kd transgenic mice, whereas expression of a control gene (Gapdh) was similar in ATR-kd transgenic mice and littermate controls (Fig. 1C). Protein expression of FLAG-tagged ATR-kd transgene was detected after enrichment by immunoprecipitation only in transgenic mice (Fig. 1D). We concluded that protein expression of the FLAG-tagged transgene was low because (i) total ATR protein bands were similar between keratinocytes derived from transgene-negative littermate controls and from ATR-kd transgenic mice (Fig. 1D) and (ii) FLAG-tagged ATR could not be detected without immunoprecipitation (Fig. 1D, Input vs. IP:αFLAG).

ATR-kd Transgene Expression in Keratinocytes Inhibits UV Responses and Augments UV-Induced Apoptosis via ATR–Chk1 Pathway Inhibition.

To investigate the effect of ATR-kd expression on UV responses in keratinocytes, we isolated primary mouse keratinocytes from ATR-kd transgenic mice and transgene-negative littermate controls. Primary keratinocytes from ATR-kd transgenic mice showed partial inhibition of UV-induced phosphorylation of Chk1, a downstream target of ATR, similar to inhibition by caffeine (nonspecific ATM/ATR inhibitor) (Fig. 2A).

Fig. 2.
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Fig. 2.

Primary keratinocytes from ATR-kd transgenic mice demonstrate ATR signaling inhibition and augmentation of UV-induced apoptosis. (A) ATR-kd inhibits UV-induced phosphorylation of Chk1. Primary mouse keratinocytes isolated from ATR-kd transgenic (Tg) mice and transgene-negative littermate controls (Ctrl) were treated with medium or caffeine (3 mM) for 30 min before UV irradiation. Cells were irradiated with 75 mJ/cm2 of UVB light and harvested 1 h after UV exposure for immunoblot analyses with the indicated antibodies. (B) ATR-kd does not inhibit doxorubicin-induced phosphorylation of ATM. Primary mouse keratinocytes isolated from ATR-kd transgenic (Tg) mice and transgene-negative littermate controls (Ctrl) were treated with medium or doxorubicin (1 μM) for 2 h and harvested for immunoblot analyses with the indicated antibodies. Arrowheads indicate same molecular size, corresponding to the ATM protein. (C and D) ATR-kd augments UV-induced apoptosis measured by sub-2N DNA content. Primary mouse keratinocytes were isolated from ATR-kd transgenic (Tg) mice and transgene-negative littermate controls (Ctrl). Cells were treated with medium or caffeine (3 mM) for 30 min before UV irradiation. Cells were irradiated with 75 mJ/cm2 of UVB light, harvested 24 h later, and stained with propidium iodide for flow cytometry analysis. (C) Representative DNA content patterns of cells without caffeine treatment with percentage of sub-2N DNA content indicated. (D) Mean of percentage of sub-2N DNA content is shown (n = 4). (Error bars = SEM.)

Given the known functional similarity between ATR and ATM, we wanted to determine whether ATR-kd nonspecifically inhibits ATM activation because such nonspecificity would complicate interpretation of these studies. We assessed ATM activation via phosphorylation at Ser1987 in mouse ATM (which corresponds to Ser1981 in human ATM) (30). To avoid activating ATR, we used doxorubicin, a topoisomerase II inhibitor that selectively activates ATM compared with ATR. ATR-kd did not inhibit doxorubicin-induced phosphorylation of ATM (Fig. 2B). This finding demonstrates that ATR-kd selectively inhibits ATR-mediated phosphorylation, not ATM phosphorylation.

Because DNA-damaged human keratinocytes with ATR or Chk1 inhibition undergo apoptosis (28), we examined the effect of ATR-kd transgene expression on UV-induced apoptosis in keratinocytes freshly isolated from mice. Keratinocytes from transgene-positive mice showed augmentation of UV-induced apoptosis (sub-2N DNA content of 30%) compared with littermate controls (15%) (Fig. 2C). This augmentation by ATR-kd was similar to augmentation by caffeine in transgene-negative keratinocytes (32.1%) (Fig. 2D). Addition of caffeine did not further augment UV-induced apoptosis of ATR-kd keratinocytes (28.5%). These findings suggest that caffeine's ability to augment UV-induced apoptosis was mediated through ATR inhibition.

A preliminary study on apoptotic sunburn cells in epidermis was carried out in an earlier strain (FVB genetic background) of ATR-kd transgenic mice. Unlike our primary keratinocyte studies in cells derived from Xpc−/− animals, no difference between ATR-kd transgenic mice and transgene-negative littermate controls was observed in UV-induced sunburn cell formation at 6 or 10 h after 30 mJ/cm2 of UVB light.

ATR-kd Transgenic Mice Chronically Irradiated with UV Demonstrate Longer Tumor Latency and Lower Skin Tumor Incidence.

All ATR-kd transgenic mice used for chronic UV experiments were verified for transgene mRNA expression by real-time RT-PCR. The ATR-kd transgenic mouse cohort and that of transgene-negative littermate controls were carefully matched to be comparable in age, sex, and coat color between groups. All mice were verified to have Xpc−/− genotype and were irradiated with UVB light three times a week for 40 wk (cumulative UVB dosage as shown in Fig. 3A).

Fig. 3.
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Fig. 3.

ATR-kd transgene delays tumor onset and suppresses UV tumorigenesis. (A) UV irradiation timeline indicating cumulative UVB dosage. Mice were irradiated with 250–2,500 J/m2 of UVB light per day, thrice weekly. (B) Percentage of tumor-free mice is shown for each group: ATR-kd transgenic (Tg) mice or transgene-negative littermate controls (Ctrl). All mice in both groups were Xpc−/−. Kaplan–Meier curves were compared by using the logrank test for statistical significance. (C) Tumor onset is delayed in ATR-kd transgenic mice. Mean number of weeks of UV treatment required for developing an initial tumor is shown. (Error bars = SEM.) (D) ATR-kd transgene suppresses UV-induced tumor development. Mean number of tumors per mouse is shown up to 19 wk, the point when some mice with advanced tumors were killed and the cohort was no longer complete. (Error bars = SEM.) Statistical significance in mean number of tumors per mouse between the groups was as shown at the indicated time points: *P ≤ 0.05, **P < 0.01. (E) Correlation of visible tumor features with histological tumor invasiveness in a randomly selected subset of tumors at time of euthanasia. Before histologic examination, 47 tumors (22 from ATR-kd transgenic mice and 25 from transgene-negative littermate controls) were segregated into five groups as shown based on size, bleeding, and ulceration/cratering/erosion. Larger tumors (≥3 mm) with bleeding and/or ulceration/cratering/erosion were presumed to be invasive SCCs. Indeed, all 34 tumors that had visible features associated with invasive SCCs were verified pathologically to be invasive SCCs. The 14 tumors that did not meet clinical criteria for invasive SCC were found microscopically to be SCC in situ (n = 7), keratoacanthoma (n = 5), epidermal hyperplasia (n = 1), and invasive SCC (n = 1). (F) ATR-kd transgenic mice had fourfold fewer clinically defined invasive SCCs than transgene-negative littermate controls (Ctrl) did, although this result did not meet statistical significance (P = 0.071). Mean number of invasive SCCs per mouse after 23 wk of UV treatment is shown. (Error bars = SEM.)

Transgene-negative littermate controls started developing tumors after 12 wk of UV treatment (Fig. 3B). Compared with transgene-negative littermates, ATR-kd transgene-positive mice showed significantly delayed tumor development as measured by the fraction of tumor-free mice (P = 0.0029; Fig. 3B) as well as a 3-wk delay in time of onset of the first tumor (P = 0.0064; Fig. 3C). In addition, at any given time point, the average number of tumors in ATR-kd mice was significantly lower than that in transgene-negative littermate controls (Fig. 3D). ATR-kd mice demonstrated 69% fewer tumors after 19 wk of UV treatment (P = 0.019).

ATR-kd Mice Demonstrate a Trend Toward Decreased Number of Invasive SCCs.

Although tumor latency was extended in ATR-kd mice, continued chronic UV irradiation eventually caused tumor development in all transgene-negative littermate controls and transgenic mice by 34 wk of UV treatment. Tumors that developed in these Xpc−/− mice in the presence or absence of the ATR-kd transgene were mainly keratoacanthomas and SCCs. These lesions were similar to those previously described (29). Tumors that developed in ATR-kd mice were histologically indistinguishable from those in transgene-negative littermate controls, suggesting that ATR-kd did not affect the development of specific types of tumors and did not change the tumor spectrum.

Continued UV irradiation promotes invasive progression of tumors. We developed criteria for clinically identifying invasive SCCs in UV-irradiated mice to monitor tumor progression by visual inspection without biopsy (which would eliminate developing tumors). The criteria were based on three visible tumor features: (i) ≥3-mm tumor diameter; (ii) bleeding from a tumor; and (iii) ulceration, cratering, or erosion of a tumor. The clinical definition of invasive SCC required that a tumor be ≥3 mm and also display bleeding, ulceration/cratering/erosion, or both. Histological analyses of tumors on UV-irradiated mice revealed that these clinical criteria for invasive SCC strongly correlated with histological diagnosis (Fig. 3E). Tumors without bleeding or ulceration/cratering/erosion were in situ tumors in 13 of 14 specimens, regardless of tumor diameter. All tumors that met the clinical criteria for invasive SCCs were in fact histologically invasive SCCs (33 of 33 tumors).

Using these validated visual criteria for invasive SCC, we monitored the emergence of these tumors during chronic UV treatment. We found that ATR-kd mice developed four times fewer invasive SCCs than transgene-negative littermate controls did (0.08 vs. 0.33 invasive SCCs per mouse; Fig. 3F), although statistical power was not sufficient to reach significance between the two groups (P = 0.071). We also measured latency from initial tumor development to clinically defined invasive SCC. Among all tumors that ultimately progressed to clinically defined invasive SCCs (n = 142 with 68 in transgenic mice and 74 in transgene-negative littermate controls), the average latency from initial tumor detection to invasive SCC development was 22.2 ± 1.8 d (mean ± SEM). The latency in transgenic mice (18.5 ± 2.3 d, n = 68) was somewhat shorter than that in the transgene-negative littermate controls (25.6 ± 2.8 d, n = 74), a difference that approached statistical significance (P = 0.052).

Discussion

To investigate the effect of ATR inhibition on UV-induced skin carcinogenesis, we created a mouse model in which ATR is partially inhibited in skin. In analogy to other DNA damage response checkpoint proteins, ATR inhibition might be expected to increase genomic instability, promoting tumorigenesis. Instead, we found that ATR-kd transgenic mice were protected from UV carcinogenesis. Presence of the ATR-kd transgene mimicked the effects of caffeine and promoted apoptosis of DNA-damaged keratinocytes shortly after UV exposure. This study suggests that caffeine's UV protective effect, documented in previous human epidemiologic and mouse in vivo studies, is likely attributable to ATR inhibition. This plausible mechanism could be further validated by examining whether caffeine demonstrates an additive effect on skin cancer prevention in ATR-kd mice. Although this experiment cannot be performed in vivo because of cost, it appears that caffeine administration to ATR-kd transgenic mice would be unlikely to show further protection from UV-induced skin tumors because there was no additive effect of caffeine on UV-induced apoptosis in transgenic keratinocyte experiments (Fig. 2D).

Previous studies have shown that the DNA damage response is an important anticancer barrier in early tumorigenesis to cope with aberrant oncogenic stimuli and to maintain genomic integrity (31, 32). Typically, inhibiting oncogene-induced DNA damage responses via the ATM–Chk2 pathway facilitates tumor development (33–35). Furthermore, caffeine administration or ATR suppression promotes oncogenic Ras-induced tumorigenesis (33, 36). However, in the present study, we found that ATR inhibition provided protection from UV tumorigenesis. Ras mutations are rare in UV-induced mouse skin tumors (37). What might explain the contrasting effects of ATR inhibition on Ras-induced tumorigenesis compared with UV-induced tumorigenesis? One plausible explanation is that ATR inhibition preferentially works at a precancerous stage to eliminate UV DNA-damaged cells through augmentation of apoptosis (possibly before oncogenes are activated, conferring survival advantage), preventing subsequent progression to UV-induced skin cancers. In contrast, after oncogenes have already been activated and cells suffer hyper-replicative stress, as in Ras-induced tumorigenesis, inhibition of DNA damage responses such as the ATM/ATR pathways may more likely promote genomic instability and hence tumorigenesis.

Chronic UV exposure eventually led to tumors in mice with diminished ATR function in skin, although tumor onset was significantly delayed in transgenic compared with littermate control mice. In contrast, the time required to progress from an initial tumor to an invasive SCC tended to be shorter in transgenic mice than that in littermate control mice. These findings suggest that ATR suppression diminished the early stages of UV carcinogenesis but not the progression from initial tumor to invasive SCC. Once keratinocytes acquire genetic mutations required for tumor cell survival and development, continued ATR suppression may no longer be effective in decreasing tumor development. Another possibility is that transgene expression was reduced in established tumors, explaining the lack of an effect on the rate of progression to aggressive tumors.

For prevention of UV-induced SCCs, p53-mutant keratinocytes are important targets because UV frequently induces p53 mutations in epidermal keratinocytes and SCCs often originate from p53-mutant keratinocytes (38). ATR inhibition can selectively sensitize G1 checkpoint-deficient cells to UV (16). Also, ATR knockdown in adult mice showed synthetic lethality with p53 deficiency, suggesting that ATR inhibition may selectively eradicate p53-deficient cells (39). Indeed, topical application of caffeine on mouse skin augmented UV-induced apoptotic sunburn cells not only in p53+/+ but also in p53−/− mice (40). Thus, it appears that ATR inhibition sensitizes any DNA-damaged cell type to death but that p53-mutant cells are especially susceptible to ATR inhibition.

We carried out a preliminary experiment to detect sunburn cells in ATR-kd mice (in the FVB genetic background with wild-type XPC function) treated with a single dose (30 mJ/cm2) of UVB light. This study did not reveal differences between transgenic mice and transgene-negative littermate controls, whereas keratinocyte experiments in the present study show that ATR-kd augments UV-induced apoptosis. Our previous studies also demonstrated that caffeine (possibly via ATR inhibition) augments UV-induced apoptosis in vivo (as well as in vitro) (9, 10). One explanation for these differing results is that experimental conditions, such as UV dose and the timing of sunburn cell detection after UV exposure, might have been suboptimal. Rates of formation of sunburn cells depend on UV dose, timing after UV exposure, and genetic background (40). In hairless Xpc−/− mice, apoptotic cells (caspase 3-positive) were observed 24 h after 200 mJ/cm2 of UVB light (41). Another possibility is that the expression level of ATR-kd transgene under the K14 promoter was extremely low in vivo, leading to a very small effect of ATR-kd on an acute experiment, such as sunburn cell detection after a single dose of UV. However, ATR-kd could have an effect on a cumulative basis, such as in carcinogenesis.

Outside of the observed preventive effect of ATR suppression on UV tumorigenesis, the ATR-kd transgenic mice showed no survival disadvantage, skin abnormalities, or spontaneous tumor development without UV treatment. These observations of tissue-specific inhibition of ATR function differ from previous studies of humans and mice with diminished ATR function. A splicing mutation in the ATR gene, causing reduced expression of ATR, is linked to human patients with Seckel syndrome characterized by bird-headed dwarfism (26). ATR hypomorphic mice with Seckel mutation showed accelerated aging and died within 6 mo after birth (21). Conditional ATR knockdown in adult mice also showed accelerated aging (42). Our results suggest that low-level, tissue-specific inhibition of the replication checkpoint is well tolerated.

SKH-1 hairless mice have been widely used as an animal model for skin cancer development because these mice are highly susceptible to UV and develop papillomas, keratoacanthomas, and SCCs after chronic UV irradiation (43, 44). Shaving wild-type haired mice does not make them as susceptible to UV as SKH-1 mice are (37), and the mechanisms of susceptibility of SKH-1 mice to UV are not well understood. Indeed, in humans, a missense mutation in the hairless gene is associated with complete hair loss but is not linked to UV susceptibility (45), suggesting species-specific differences in the roles for this protein. For the present study, we selected a well-characterized UV-susceptible model, Xpc−/− mice, that develop keratoacanthomas and SCCs after chronic UV irradiation because of DNA-repair deficiency (29). Unlike other DNA-repair deficiency models (Xpa−/− and Csb−/−) in which UV-induced apoptosis is markedly augmented, Xpc−/− mice demonstrate similar rates of UV-induced apoptosis compared with wild-type mice (41), making this model appropriate for investigating effects of ATR inhibition on augmentation of UV-induced apoptosis. Although xeroderma pigmentosum is a rare genetic disease, XPC protein expression is lost in up to 59% of invasive SCCs from non-XPC patients (46), further increasing the relevance of this model to human skin cancer studies. Beyond the persistence of a larger amount of DNA damage after UV exposure in XPC-deficient versus wild-type cells, the subsequent progression to malignancy is not thought to differ between XPC-deficient and wild-type cells.

Although inhibiting ATM/ATR pathways promotes tumorigenesis in other settings, our findings from ATR-kd transgenic mice suggest that ATR inhibition in skin is well tolerated and suppresses UV-induced tumor development. Combined with the extensive epidemiologic data linking caffeine intake with decreased skin cancer development, these findings suggest the possibility that topical caffeine application could be useful in preventing UV-induced skin cancers. An additional appealing aspect of topical application of caffeine is that it directly absorbs UV and thus also acts as a sunscreen, potentiating the efficacy of topical UV protection (47). This chemopreventive approach via ATR inhibition might be especially beneficial for individuals at high risk of UV-associated nonmelanoma skin cancers.

Materials and Methods

Generation of ATR-kd Transgenic Mice.

Amino-terminal FLAG epitope-tagged full-length construct of human ATR-kd was generated as previously described (16, 24). The construct was subcloned into the human K14 promoter construct containing an intron from the β-globin gene (23). The full expression cassette of K14–ATR-kd was then digested away from plasmid DNA sequences before injection into mouse blastocysts.

ATR-kd transgenic mice were generated at the Brigham and Women's Hospital Transgenic Mouse Core Facility with the FVB mouse strain. Presence of the ATR-kd transgene was confirmed by PCR using genomic DNA extracted from mouse tails and primers that amplified the human K14 promoter (only present in transgenic mice). A section of mouse bone morphogenic protein receptor type II (Bmpr2) was used as control for DNA quality in PCR experiments.

ATR-kd transgenic mice in an FVB genetic background were crossed with Xpc−/− mice in a mixed genetic background that contained both C57BL/6 and 129S7 (B6;129S7-Xpctm1Brd, model no. 000557; Taconic). The offspring were intercrossed to maintain colonies and obtain ATR-kd transgenic mice and transgene-negative littermate controls. All mice used in this study were Xpc−/− mice with or without the ATR-kd transgene. Xpc−/− genotype was confirmed by PCR to demonstrate no amplification at two loci: Xpc exon 3 and exon 4.

PCR for genotyping and real-time RT-PCR for detecting ATR-kd transgene expression are described in SI Materials and Methods.

Chronic UV Irradiation of Mice.

All animal procedures were approved by the Institutional Animal Care and Use Committee at the University of Washington. Before chronic UV treatment, 11- to 44-wk-old ATR-kd transgenic mice (n = 43) and their transgene-negative littermate controls (n = 43) were selected from among larger populations so that the final groups were closely matched for age, sex, and coat color. During chronic UV treatment, mice were shaved once per week. Mice were irradiated with UVB light from FS40T12/UVB bulbs (National Biological Corporation) with peak emission between 300 and 315 nm and rapid decrease in emission beyond the UVB range (UV below 280 nm was undetectable). UVB dose was measured with a Photolight IL1400A radiometer equipped with a SEL240/UVB detector (International Light Technologies). Mice were treated with 250–2,500 J/m2 of UVB light per day, typically thrice weekly. UV treatment was temporarily decreased or held if >20% of mice developed significant skin erythema. UV treatment continued until all mice developed at least one tumor, with the final cumulative dose of UVB light at the end of the experiment (282 d) being 166.8 kJ/m2. Mice were monitored weekly for the development of tumors on ears and back skin. A discrete, palpable, and persistent papule was recorded as an initial tumor. An invasive SCC was defined as a larger tumor (≥3-mm tumor diameter) with evidence of bleeding and/or ulceration/cratering/erosion. Mice were euthanized if they had any of the following: a tumor ≥1 cm in diameter, persistent bleeding of any tumor, or cachexia. A description of histological analyses of tumors is provided in SI Materials and Methods.

Statistical Analyses.

Statistical significance in mean between two groups was determined by a two-tailed, two-sample t test. Error bars in figures indicate SEM. Kaplan–Meier curves (Fig. 3B) were compared by using the logrank test for statistical significance (Prism 5; GraphPad Software). A detailed description of mice excluded from the tumor analyses shown in Fig. 3 C, D, and F (because of deaths unrelated to skin tumors) is included in SI Materials and Methods.

Isolation of Primary Keratinocytes from Transgenic Mice.

Primary mouse keratinocytes were isolated from the epidermis of 1- to 3-d-old transgenic mice and transgene-negative littermate controls, as previously described (48). Detailed methods for experiments using isolated primary keratinocytes are provided in SI Materials and Methods.

Acknowledgments

We thank Dr. Elaine Fuchs for providing the human β-globin intron-containing keratin-14 promoter construct. This work was supported by National Institutes of Health Grants R01-AR049832 (to P.N.) and R01-CA130857 (to A.H.C.).

Footnotes

  • 1To whom correspondence may be addressed. E-mail: aconney{at}pharmacy.rutgers.edu or pnghiem{at}uw.edu.
  • Author contributions: M.K., B.L., J.E.B., Y.-s.K., A.H.C., and P.N. designed research; M.K., B.L., J.E.B., R.T., Y.-s.K., M.S., E.H., S.-w.K., A.A.-Z., A.C., D.L., L.N., T.P.H., I.L., A.M., Y.-P.L., and P.N. performed research; M.K., B.L., J.E.B., R.T., M.S., E.H., Y.-P.L., A.H.C., and P.N. analyzed data; and M.K. and P.N. wrote the paper.

  • The authors declare no conflict of interest.

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1111378108/-/DCSupplemental.

Freely available online through the PNAS open access option.

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Protection from UV-induced skin carcinogenesis by genetic inhibition of the ataxia telangiectasia and Rad3-related (ATR) kinase
Masaoki Kawasumi, Bianca Lemos, James E. Bradner, Renee Thibodeau, Yong-son Kim, Miranda Schmidt, Erin Higgins, Sang-wahn Koo, Aimee Angle-Zahn, Adam Chen, Douglas Levine, Lynh Nguyen, Timothy P. Heffernan, Isabel Longo, Anna Mandinova, Yao-Ping Lu, Allan H. Conney, Paul Nghiem
Proceedings of the National Academy of Sciences Aug 2011, 108 (33) 13716-13721; DOI: 10.1073/pnas.1111378108

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Protection from UV-induced skin carcinogenesis by genetic inhibition of the ataxia telangiectasia and Rad3-related (ATR) kinase
Masaoki Kawasumi, Bianca Lemos, James E. Bradner, Renee Thibodeau, Yong-son Kim, Miranda Schmidt, Erin Higgins, Sang-wahn Koo, Aimee Angle-Zahn, Adam Chen, Douglas Levine, Lynh Nguyen, Timothy P. Heffernan, Isabel Longo, Anna Mandinova, Yao-Ping Lu, Allan H. Conney, Paul Nghiem
Proceedings of the National Academy of Sciences Aug 2011, 108 (33) 13716-13721; DOI: 10.1073/pnas.1111378108
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