Skip to main content

Main menu

  • Home
  • Articles
    • Current
    • Special Feature Articles - Most Recent
    • Special Features
    • Colloquia
    • Collected Articles
    • PNAS Classics
    • List of Issues
  • Front Matter
    • Front Matter Portal
    • Journal Club
  • News
    • For the Press
    • This Week In PNAS
    • PNAS in the News
  • Podcasts
  • Authors
    • Information for Authors
    • Editorial and Journal Policies
    • Submission Procedures
    • Fees and Licenses
  • Submit
  • Submit
  • About
    • Editorial Board
    • PNAS Staff
    • FAQ
    • Accessibility Statement
    • Rights and Permissions
    • Site Map
  • Contact
  • Journal Club
  • Subscribe
    • Subscription Rates
    • Subscriptions FAQ
    • Open Access
    • Recommend PNAS to Your Librarian

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Home
Home
  • Log in
  • My Cart

Advanced Search

  • Home
  • Articles
    • Current
    • Special Feature Articles - Most Recent
    • Special Features
    • Colloquia
    • Collected Articles
    • PNAS Classics
    • List of Issues
  • Front Matter
    • Front Matter Portal
    • Journal Club
  • News
    • For the Press
    • This Week In PNAS
    • PNAS in the News
  • Podcasts
  • Authors
    • Information for Authors
    • Editorial and Journal Policies
    • Submission Procedures
    • Fees and Licenses
  • Submit
Research Article

Immunization of fucose-containing polysaccharides from Reishi mushroom induces antibodies to tumor-associated Globo H-series epitopes

Shih-Fen Liao, Chi-Hui Liang, Ming-Yi Ho, Tsui-Ling Hsu, Tsung-I Tsai, Yves S.-Y. Hsieh, Chih-Ming Tsai, Shiou-Ting Li, Yang-Yu Cheng, Shu-Ming Tsao, Tung-Yi Lin, Zong-Yan Lin, Wen-Bin Yang, Chien-Tai Ren, Kuo-I Lin, Kay-Hooi Khoo, Chun-Hung Lin, Hsien-Yeh Hsu, Chung-Yi Wu, and Chi-Huey Wong
  1. aInstitute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan;
  2. bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
  3. cInstitute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan;
  4. dInstitute of Biotechnology, National Taiwan University, Taipei 106, Taiwan;
  5. eDepartment of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, Taiwan; and
  6. fProgram in Molecular Medicine, National Yang-Ming University, Taipei 112, Taiwan

See allHide authors and affiliations

PNAS August 20, 2013 110 (34) 13809-13814; https://doi.org/10.1073/pnas.1312457110
Shih-Fen Liao
aInstitute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan;
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chi-Hui Liang
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ming-Yi Ho
cInstitute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tsui-Ling Hsu
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tsung-I Tsai
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
dInstitute of Biotechnology, National Taiwan University, Taipei 106, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yves S.-Y. Hsieh
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chih-Ming Tsai
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shiou-Ting Li
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yang-Yu Cheng
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shu-Ming Tsao
eDepartment of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, Taiwan; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tung-Yi Lin
fProgram in Molecular Medicine, National Yang-Ming University, Taipei 112, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zong-Yan Lin
cInstitute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wen-Bin Yang
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chien-Tai Ren
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kuo-I Lin
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kay-Hooi Khoo
cInstitute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chun-Hung Lin
cInstitute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hsien-Yeh Hsu
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
eDepartment of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, Taiwan; and
fProgram in Molecular Medicine, National Yang-Ming University, Taipei 112, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: hsienyeh@gmail.com cyiwu@gate.sinica.edu.tw chwong@gate.sinica.edu.tw
Chung-Yi Wu
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: hsienyeh@gmail.com cyiwu@gate.sinica.edu.tw chwong@gate.sinica.edu.tw
Chi-Huey Wong
bGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: hsienyeh@gmail.com cyiwu@gate.sinica.edu.tw chwong@gate.sinica.edu.tw
  1. Contributed by Chi-Huey Wong, July 3, 2013 (sent for review May 16, 2013)

  • Article
  • Figures & SI
  • Info & Metrics
  • PDF
Loading

Abstract

Carbohydrate-based vaccines have shown therapeutic efficacy for infectious disease and cancer. The mushroom Ganoderma lucidum (Reishi) containing complex polysaccharides has been used as antitumor supplement, but the mechanism of immune response has rarely been studied. Here, we show that the mice immunized with a l-fucose (Fuc)-enriched Reishi polysaccharide fraction (designated as FMS) induce antibodies against murine Lewis lung carcinoma cells, with increased antibody-mediated cytotoxicity and reduced production of tumor-associated inflammatory mediators (in particular, monocyte chemoattractant protein-1). The mice showed a significant increase in the peritoneal B1 B-cell population, suggesting FMS-mediated anti-glycan IgM production. Furthermore, the glycan microarray analysis of FMS-induced antisera displayed a high specificity toward tumor-associated glycans, with the antigenic structure located in the nonreducing termini (i.e., Fucα1-2Galβ1-3GalNAc-R, where Gal, GalNAc, and R represent, respectively, D-galactose, D-N-acetyl galactosamine, and reducing end), typically found in Globo H and related tumor antigens. The composition of FMS contains mainly the backbone of 1,4-mannan and 1,6-α-galactan and through the Fucα1-2Gal, Fucα1-3/4Man, Fucα1-4Xyl, and Fucα1-2Fuc linkages (where Man and Xyl represent d-mannose and d-xylose, respectively), underlying the molecular basis of the FMS-induced IgM antibodies against tumor-specific glycans.

  • mushroom polysaccharide
  • antitumor activity
  • anti-Globo H antibody

Footnotes

  • ↵1S.-F.L. and C.-H. Liang contributed equally to this work.

  • ↵2To whom correspondence may be addressed. E-mail: hsienyeh{at}gmail.com, cyiwu{at}gate.sinica.edu.tw, or chwong{at}gate.sinica.edu.tw.
  • Author contributions: H.-Y.H., C.-Y.W., and C.-H.W. designed research; S.-F.L., C.-H. Liang, M.-Y.H., S.-T.L., Y.-Y.C., S.-M.T., T.-Y.L., Z.-Y.L., W.-B.Y., and C.-T.R. performed research; T.-I.T. and Y.S.-Y.H. contributed new reagents/analytic tools; S.-F.L., C.-H. Liang, M.-Y.H., T.-L.H., Y.S.-Y.H., C.-M.T., and K.-I.L. analyzed data; and S.-F.L., C.-H. Liang, K.-H.K., C.-H. Lin, H.-Y.H., C.-Y.W., and C.-H.W. wrote the paper.

  • The authors declare no conflict of interest.

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1312457110/-/DCSupplemental.

View Full Text
PreviousNext
Back to top
Article Alerts
Email Article

Thank you for your interest in spreading the word on PNAS.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Immunization of fucose-containing polysaccharides from Reishi mushroom induces antibodies to tumor-associated Globo H-series epitopes
(Your Name) has sent you a message from PNAS
(Your Name) thought you would like to see the PNAS web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Reishi mushroom polysaccharides
Shih-Fen Liao, Chi-Hui Liang, Ming-Yi Ho, Tsui-Ling Hsu, Tsung-I Tsai, Yves S.-Y. Hsieh, Chih-Ming Tsai, Shiou-Ting Li, Yang-Yu Cheng, Shu-Ming Tsao, Tung-Yi Lin, Zong-Yan Lin, Wen-Bin Yang, Chien-Tai Ren, Kuo-I Lin, Kay-Hooi Khoo, Chun-Hung Lin, Hsien-Yeh Hsu, Chung-Yi Wu, Chi-Huey Wong
Proceedings of the National Academy of Sciences Aug 2013, 110 (34) 13809-13814; DOI: 10.1073/pnas.1312457110

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
Reishi mushroom polysaccharides
Shih-Fen Liao, Chi-Hui Liang, Ming-Yi Ho, Tsui-Ling Hsu, Tsung-I Tsai, Yves S.-Y. Hsieh, Chih-Ming Tsai, Shiou-Ting Li, Yang-Yu Cheng, Shu-Ming Tsao, Tung-Yi Lin, Zong-Yan Lin, Wen-Bin Yang, Chien-Tai Ren, Kuo-I Lin, Kay-Hooi Khoo, Chun-Hung Lin, Hsien-Yeh Hsu, Chung-Yi Wu, Chi-Huey Wong
Proceedings of the National Academy of Sciences Aug 2013, 110 (34) 13809-13814; DOI: 10.1073/pnas.1312457110
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Mendeley logo Mendeley

Article Classifications

  • Biological Sciences
  • Biochemistry
Proceedings of the National Academy of Sciences: 110 (34)
Table of Contents

Submit

Sign up for Article Alerts

Jump to section

  • Article
    • Abstract
    • Results and Discussion
    • Conclusion
    • Materials and Methods
    • Acknowledgments
    • Footnotes
    • References
  • Figures & SI
  • Info & Metrics
  • PDF

You May Also be Interested in

Water from a faucet fills a glass.
News Feature: How “forever chemicals” might impair the immune system
Researchers are exploring whether these ubiquitous fluorinated molecules might worsen infections or hamper vaccine effectiveness.
Image credit: Shutterstock/Dmitry Naumov.
Reflection of clouds in the still waters of Mono Lake in California.
Inner Workings: Making headway with the mysteries of life’s origins
Recent experiments and simulations are starting to answer some fundamental questions about how life came to be.
Image credit: Shutterstock/Radoslaw Lecyk.
Cave in coastal Kenya with tree growing in the middle.
Journal Club: Small, sharp blades mark shift from Middle to Later Stone Age in coastal Kenya
Archaeologists have long tried to define the transition between the two time periods.
Image credit: Ceri Shipton.
Illustration of groups of people chatting
Exploring the length of human conversations
Adam Mastroianni and Daniel Gilbert explore why conversations almost never end when people want them to.
Listen
Past PodcastsSubscribe
Panda bear hanging in a tree
How horse manure helps giant pandas tolerate cold
A study finds that giant pandas roll in horse manure to increase their cold tolerance.
Image credit: Fuwen Wei.

Similar Articles

Site Logo
Powered by HighWire
  • Submit Manuscript
  • Twitter
  • Facebook
  • RSS Feeds
  • Email Alerts

Articles

  • Current Issue
  • Special Feature Articles – Most Recent
  • List of Issues

PNAS Portals

  • Anthropology
  • Chemistry
  • Classics
  • Front Matter
  • Physics
  • Sustainability Science
  • Teaching Resources

Information

  • Authors
  • Editorial Board
  • Reviewers
  • Subscribers
  • Librarians
  • Press
  • Cozzarelli Prize
  • Site Map
  • PNAS Updates
  • FAQs
  • Accessibility Statement
  • Rights & Permissions
  • About
  • Contact

Feedback    Privacy/Legal

Copyright © 2021 National Academy of Sciences. Online ISSN 1091-6490