Isotopic signals of summer denitrification in a northern hardwood forested catchment

Sarah K. Wexler; Christine L. Goodale; Kevin J. McGuire; Scott W. Bailey; Peter M. Groffman

doi:10.1073/pnas.1404321111

Edited by Thure E. Cerling, University of Utah, Salt Lake City, UT, and approved October 8, 2014 (received for review March 7, 2014)

Significance

Denitrification is the most poorly understood process in the terrestrial N cycle. As a result, terrestrial N budgets are wildly unbalanced and our ability to address global nitrogen pollution is fundamentally constrained. Denitrification is controlled by multiple factors, often exhibiting extraordinary variation in time and space, especially in terrestrial environments. Temperate forests regularly receive much larger inputs of precipitation N than they export in streamwater. The fate of the rest has been elusive. We present stable isotope measurements revealing extensive evidence of denitrification from temperate-forest shallow groundwater in midsummer, even as concurrent measurements of streamwater show little sign of denitrification. These measurements support the importance of a disputed nitrogen removal process and its occurrence at a previously missed time and location.

Abstract

Despite decades of measurements, the nitrogen balance of temperate forest catchments remains poorly understood. Atmospheric nitrogen deposition often greatly exceeds streamwater nitrogen losses; the fate of the remaining nitrogen is highly uncertain. Gaseous losses of nitrogen to denitrification are especially poorly documented and are often ignored. Here, we provide isotopic evidence (δ¹⁵N_NO3 and δ¹⁸O_NO3) from shallow groundwater at the Hubbard Brook Experimental Forest indicating extensive denitrification during midsummer, when transient, perched patches of saturation developed in hillslopes, with poor hydrological connectivity to the stream, while streamwater showed no isotopic evidence of denitrification. During small rain events, precipitation directly contributed up to 34% of streamwater nitrate, which was otherwise produced by nitrification. Together, these measurements reveal the importance of denitrification in hydrologically disconnected patches of shallow groundwater during midsummer as largely overlooked control points for nitrogen loss from temperate forest catchments.

Many forested catchments export far less nitrogen (N) in streamwater than they receive in atmospheric deposition (1, 2). The rest of the deposited N may accumulate in vegetation or soil organic matter, or be lost in gaseous form. Losses of N to denitrification, the microbial reduction of aqueous nitrate (NO₃⁻) to nitrous oxide (N₂O, a greenhouse gas) and N₂ gas, are extremely difficult to measure due to the difficulty in directly measuring N₂ fluxes and due to the high degree of spatiotemporal variability in redox conditions and substrate sources (3). Many past studies using a range of measurements (streamwater nitrate isotopic composition, the acetylene block technique, N₂O emissions, and mass balance calculations) have concluded that denitrification in temperate forests is highly uncertain or generally unimportant (e.g., refs. 4 ⇓⇓⇓–8).

Nitrogen budgets are particularly perplexing in the northern hardwood forests at the Hubbard Brook Experimental Forest (HBEF) in the White Mountains of New Hampshire, USA, where atmospheric deposition has supplied 6–8 kg N ha⁻¹⋅yr⁻¹ for half a century, a rate ∼5–10 times preindustrial levels (7 ⇓⇓–10). Accumulation of N in plant biomass ceased in the early 1990s (10, 11), while streamwater inorganic N export from catchments across the HBEF and nearby streams decreased to <1 kg N ha⁻¹⋅yr⁻¹, for reasons that remain elusive (9, 10, 12). These N flux measurements imply increasingly important roles for N gas loss or storage in soil organic matter. However, both processes are so difficult to quantify that the fate, drivers, and consequences of the “missing” N remain unknown, at the HBEF and elsewhere (8 ⇓–10, 12).

Measurement of the dual isotopic composition of NO₃⁻ (δ¹⁵N_NO3 and δ¹⁸O_NO3) provides a powerful tool to identify NO₃⁻ sources and to infer its loss to denitrification (13 ⇓⇓–16). Values of δ¹⁸O_NO3 differ greatly between NO₃⁻ in precipitation and NO₃⁻ produced by nitrification (refs. 13 ⇓⇓–16, Table S1), which is the microbial oxidation of NH₄⁺ to NO₃⁻. Measurements of δ¹⁸O_NO3 have enabled detection of direct contributions of precipitation NO₃⁻ to streamwater (Table S1), especially during snowmelt, when catchments often release large quantities of NO₃⁻ (7, 8). However, past δ¹⁸O_NO3 measurements show that nitrification—not precipitation—supplies the vast majority of NO₃⁻ in streamwater at the HBEF (10, 17, 18) and other forested catchments (refs. 14 and 19, Table S1).

Nitrate isotopic composition reflects not only NO₃⁻ sources but also fractionation from a range of processes (14, 20). During denitrification, heterotrophic microbes consume organic carbon using NO₃⁻ as an electron acceptor under low-oxygen conditions, in a 5:4 molar ratio of carbon:NO₃⁻. If NO₃⁻ is not replenished or consumed by other processes, denitrification progressively enriches both ¹⁸O and ¹⁵N in the residual NO₃⁻, with an O:N fractionation ratio of 0.4–0.7 in the field (14, 20, 21) and up to 1.0 in laboratory studies (22). The fractionation ratio is the slope of the relationship between δ¹⁸O_NO3 and δ¹⁵N_NO3. Dual isotopic enrichment and these enrichment ratios provide evidence of denitrification. Dual isotope analysis of NO₃⁻ has provided evidence for denitrification in large aquifers (e.g., ref. 20) and in drainage waters receiving heavy agricultural N loads (e.g., refs. 21 and 23), whereas recent catchment studies in a subtropical forest (15) and a warm Mediterranean grassland (24) have reported isotopic evidence of denitrification in soil or groundwater. However, dozens of past studies of stream δ¹⁸O_NO3 and δ¹⁵N_NO3 in naturally vegetated temperate and boreal catchments (refs. 14 and 19, Table S1), including the HBEF (10, 17), have revealed little if any isotopic evidence of denitrification.

To investigate the role of denitrification at the HBEF, we measured NO₃⁻ isotopic composition throughout watershed 3 (WS3), a hydrologic reference catchment drained by Paradise Brook within the HBEF (Fig. 1), during the first two weeks of July 2011, close to the warmest part of the year (Fig. S1). Sampling encompassed nine shallow groundwater wells, a seep, and 19 stream sites along Paradise Brook and its tributaries. The shallow groundwater wells accessed water from saturated soil within the solum above the C horizon at depths between 30 and 115 cm. Three of the nine wells were close to (<2 m) or within the perennial stream channel; the other six were more distal (≥4 m) and upgradient from the perennial channel. Four rain events occurred during the sampling period (0.8–12.3 mm, 26.8 mm total); all contained NO₃⁻ and NH₄⁺ concentrations that exceeded those in streamwater and groundwater by an order of magnitude (Table 1 and Fig. 2 A and B). Nitrogen export over the study period amounted to 0.006 ± 0.003 kg N ha⁻¹, consisting of 24% NO₃⁻, 13% NH₄⁺, and 63% dissolved organic nitrogen (DON). Streamflow over the sampling period (5.3 mm) exported less than 2% of rainfall N input (0.335 ± 0.087 kg N ha⁻¹). The remaining 98% was retained within the catchment or lost via denitrification. If this 98% were denitrified in soil or shallow groundwater, it gives a maximum denitrification rate of 5.0 (3.6–6.4) kg N ha⁻¹ if extrapolated over the growing season. Although this figure represents the maximum loss of N to denitrification, recent extrapolations of N₂ and N₂O flux measurements from soil cores from the HBEF found denitrification rates during the growing season higher than previous estimates and equal to or higher than atmospheric deposition while follow-up measurements found rates ranging from 4–10 kg N ha⁻¹⋅y⁻¹ at HBEF (25).

Fig. 1.

Location of HBEF in the northeast United States (Top Right), showing HBEF watersheds 1–9 (Bottom Right) and watershed 3 (WS3; Left). WS3 shows drainage network comprising Paradise Brook and tributary channels, with sampling locations from the weir (triangle), streams (filled circles), wells (empty circles; including wells ≥4 m from surface streamflow in July 2011 (JD05, JD17, JD18, JD19, JD29, JD30) and those <2 m (R12, east bank; R13 in-stream; R14, west bank) and a seep (dash). Contour interval is 3 m; elevation range is 537–732 m.

View this table:

Table 1.

Concentrations of NO₃⁻, NH₄⁺, DON, and DOC (μM), and δ¹⁵N_NO3 and δ¹⁸O_NO3 of water samples from watershed 3, Hubbard Brook Experimental Forest, New Hampshire July 2011

Fig. 2.

Temporal pattern of nitrate concentration (A and B), δ¹⁵N_NO3 (C and D), and δ¹⁸O_NO3 (E and F) for rainfall and streams (A, C, and E) and for wells (≥4 m from surface flow) and the seep (B, D, and F). Symbols are denoted in A and B for each site type. The asterisk in E denotes an estimated rainfall δ¹⁸O_NO3 value, as the average of adjacent dates. Isotopic values are expressed per mil (‰) relative to established standards, Vienna Standard Mean Ocean Water (VSMOW) for δ¹⁸O and air for δ¹⁵N.

What Is the Source of Streamwater NO₃⁻?

Most streamwater NO₃⁻ in WS3 appeared to have been produced by microbial nitrification (Fig. 3A). That is, stream δ¹⁵N_NO3 and δ¹⁸O_NO3 values fell within the expected range for a nitrification source (see Methods), similar to past measurements in forested catchments (refs. 14 and 19, Table S1). Streamwater δ¹⁸O_NO3 values averaged −3.3‰ at baseflow (Table 1), slightly lighter than the theoretically expected value (14, 26, 27) of microbially produced NO₃⁻ at this site of 1.7 ± 0.1‰, as the combination of one oxygen atom from air (δ¹⁸O_O2 = 23.5‰) and two from local water (δ¹⁸O_H2O = −9.2 ± 0.2‰). This difference is likely due to well-documented kinetic isotope effects during nitrite oxidation and incorporation of water by nitrite reductase, and exchange of oxygen between NO₂⁻ and water during nitrification (16, 28 ⇓–30), resulting in consistently low δ¹⁸O_NO3 values in streamwater at baseflow (Fig. 3A). Nitrification occurs in aerobic soils and in the stream itself. Previous studies of stream additions of NH₄⁺ below the weir at WS3 (31, 32) and ¹⁵NH₄⁺ elsewhere at the HBEF (33) show rapid in-stream NH₄⁺ uptake, with uptake lengths of <20–61 m during baseflow conditions. In-stream nitrification can account for 10–100% of NH₄⁺ uptake in WS3, and in-stream NO₃⁻ uptake equals or exceeds in-stream nitrification (32). This past work demonstrates that in-stream recycling rapidly produces and consumes stream NO₃⁻, processes that can alter stream NO₃⁻ isotopic composition to strongly reflect an in-stream nitrification source (19).

Fig. 3.

Isotopic composition of nitrate (δ¹⁵N_NO3 and δ¹⁸O_NO3) samples collected during July 2011 in watershed 3, Hubbard Brook, New Hampshire, USA. Measurements are shown from (A) rain and streamwater separated by date between 3 July (open circles) and 4–14 July (closed circles); (B) wells ≥4 m from surface streamflow, displayed by well, with regression line determined from samples excluding well JD05 on 12 July (solid line) extrapolated to this point (dashed); (C) wells <2 m (black) displayed by well, adjacent stream samples (gray), and the denitrification line determined in B. Dashed boxes indicate the ranges of isotopic composition of two nitrate sources, rain and microbial nitrification (see Methods for explanation). Isotopic values are expressed per mil (‰) relative to established standards, VSMOW for δ¹⁸O and air for δ¹⁵N.

Not all stream δ¹⁸O_NO3 fell within the expected range for a nitrification source, particularly during or immediately after storm events. On 3 July 2011, all streamwater samples had substantially elevated δ¹⁸O_NO3 values (17.3 ± 3.3‰), reflecting a small (3.7 mm) rainfall event that rapidly contributed NO₃⁻ to the stream (Figs. 2 C and E and 3A). A two end-member mixing calculation using δ¹⁸O_NO3 (see SI Text) indicated that rainfall contributed 29–34% of stream NO₃⁻ at the WS3 weir on 3 July, and 5–16% of stream NO₃⁻ from an event (9.1 mm) on 9 July. Precipitation-derived NO₃⁻ in streamwater in headwater catchments is typically detected only during snowmelt and is rarely observed during the growing season (Table S1). These results confirm that streams can export pulses of atmospheric nitrate unaltered by microbial cycling even during summer. At WS3, streamwater export of atmospherically derived NO₃⁻ amounted to just 0.3% of the estimated NO₃⁻ flux that rained onto the catchment during the 3 July event and 0.1–0.3% of the 9 July event (see SI Text). The entire WS3 channel network, including dry channels, spans 1.8% of the catchment area (34), such that the estimated flux of NO₃⁻ that rained onto this channel area more than sufficed to supply the rainfall-derived NO₃⁻ observed in stream export at the weir, even if >80% of this channel area were dry during mid-July (see SI Text).

Groundwater Denitrification Is Consuming Soil NO₃⁻

The isotopic composition of NO₃⁻ in the six groundwater wells upgradient and away (≥4 m) from perennial streamflow showed substantial enrichment in both ¹⁵N and ¹⁸O, falling far outside the expected ranges of NO₃⁻ sources from deposition or nitrification (Table 1 and Figs. 2 D and F and 3B). Samples from these wells showed a strong positive relationship between δ¹⁸O_NO3 and δ¹⁵N_NO3 (r² = 0.68) with a slope of 0.76. This slope represents the fractionation ratio of O to N (Fig. 3B) and serves as a diagnostic of denitrification (14, 20 ⇓–22). This regression of δ¹⁸O_NO3 versus δ¹⁵N_NO3 excluded one sample (Well JD05, 12 July) that had a lighter isotopic composition and higher NO₃⁻ concentration than the others (Fig. 2 B and D), although downward extrapolation of the δ¹⁸O_NO3/δ¹⁵N_NO3 relationship directly intersected this sample point (Fig. 3B), which could represent a high-concentration NO₃⁻ end-member before isotopic fractionation by denitrification. The isotopic composition of this sample fell in the expected range for microbial nitrification and may reflect newly nitrified NO₃⁻.

If denitrification consumes NO₃⁻ in a quasi-closed system, either over time (e.g., in a pocket of water in the soil or groundwater) or through space, (e.g., in a body of water flowing along a flow path), and is the only process altering δ¹⁵N_NO3 values, the progressive consumption of a finite NO₃⁻ pool yields an increase in the δ¹⁵N of the residual NO₃⁻ in a quantitative relationship that defines an isotope enrichment factor between product and substrate (ε_p-s ¹⁵N_NO3 ‰), as approximated by a modified Rayleigh closed-system model (20). Measurements from the WS3 wells did not show a clear relationship between δ¹⁵N_NO3 and NO₃⁻ concentration, likely due to spatiotemporal heterogeneity in nitrification rates and substrate δ¹⁵N values. If denitrification was occurring in transient patches of saturated soil in the soil profile above the groundwater, sporadic pulses of partially and variably denitrified soil water may have reached each well independently. However, if the 12 July sample from well JD05 (Figs. 2 B and D and 3B) represents the NO₃⁻ concentration and δ¹⁵N_NO3 of groundwater before denitrification and the mean NO₃⁻ concentration and δ¹⁵N_NO3 of the rest of these samples represent partially denitrified NO₃⁻, denitrification produced an isotope enrichment factor of −13‰ (−5‰ to −17‰). This value falls within the literature range for field measurements of denitrification, which average −16‰ and typically range from −6‰ to −23‰ (14, 21, 35). Together, these data indicate the pervasive occurrence of denitrification in soil or shallow groundwater of NO₃⁻ that had been produced by microbial nitrification, yielding groundwater with very low NO₃⁻ concentrations (<3 μM).

The three wells in or near (<2 m) the perennial stream showed varied isotopic signals (Fig. 3C). The east well (R12) showed isotopic evidence of denitrification similar to the ≥4-m wells. That is, most samples from this well showed evidence of dual isotope fractionation with high δ¹⁵N_NO3 and δ¹⁸O_NO3 values that fell near the same denitrification line as was observed in the >4-m wells (Fig. 3 B and C). However, a few samples fell below this line. Their isotopic composition may be explained most simply as a two end-member mix of partially denitrified NO₃⁻ in groundwater and the NO₃⁻ in streamwater. Mixing these two NO₃⁻ sources would lower both δ¹⁵N_NO3 and, especially, δ¹⁸O_NO3 values in these east well samples (Fig. 3C). Mixing may be the result of a hyporheic flow path bringing water from the stream to the shallow groundwater in the riparian zone. Nitrate in the west well (R14) did not show isotopic evidence of denitrification, and nitrate in the in-stream well (R13) isotopically resembled nearby streamwater. These measurements exemplify the spatial variability of denitrification and the importance of sampling different water sources to ascertain the occurrence and magnitude of denitrification within catchments.

There have been few direct measurements of denitrification at the HBEF (4 ⇓⇓⇓–8), and the process has been considered unlikely to affect the unexplained long-term decrease in NO₃⁻ export (10). These and many other past efforts to detect denitrification in forested catchments have focused on surface soils or streams, but recent measurements of soil gases (O₂, CO_2, CH₄, N₂O) (36) and denitrification enzyme activity (37) at WS3 indicate that deeper soil horizons and shallow groundwater could potentially support significant amounts of denitrification, particularly in summer. The results here show that hotspots (38) for denitrification were identified during the sampling period in shallow groundwater or saturated soil, rather than streamwater, although the isotopic signal of denitrification may have been masked if coupled nitrification/denitrification were occurring in-stream. Denitrification hotspots may develop with the convergence of NO₃⁻ and dissolved organic carbon (DOC) produced by surface soils draining to low-oxygen zones in saturated soil and shallow groundwater. Mean growing-season concentrations of both NO₃⁻ and DOC draining from the forest floor (>20 μM NO₃⁻; >1200 μM DOC) and upper mineral soils (Bh horizons; 19–26 cm depth; 8–25 μM NO₃⁻ and 700–900 μM DOC) at the HBEF (39) are relatively high compared with the groundwater measured here (<3 μM NO₃⁻; <400 μM DOC; Table 1), and could supply both NO₃⁻ and DOC for denitrification in deeper soils or groundwater. Ratios of DOC to NO₃⁻ in all sample types in this study exceeded 5:4 by more than an order of magnitude (Table 1), indicating that carbon supply sufficed to support denitrification, even if a large fraction of the carbon was refractory. Recent measurements in WS3 show that O₂ concentrations in soil dip in summer coinciding with increases in soil respiration rate, and decrease sharply as water-filled pore space increases above ∼90% (36). This combination of substrates in low-O₂ soil or groundwater provides ideal conditions for denitrification to occur. This study provides evidence of the widespread but fragmented nature of denitrification in saturated soil and shallow groundwater in a temperate forested watershed. The existence of transient, perched patches of saturation in the soil that are poorly connected to streams (40, 41), where N then recycles rapidly (31 ⇓–33), may explain why previous dual isotope studies based on streamwater samples alone—at HBEF and elsewhere—have shown little to no denitrification signal (Table S1). That is, even as denitrification occurs in saturated patches, these hotspots are connected poorly to surface streamwater (40, 41), which contains NO₃⁻ produced by local nitrification (Fig. 3A). Hydrological disconnection between perched saturated zones and stream channels and denitrification rates may both broadly covary seasonally with the warm, drying conditions of summer (42), creating fragmented patches of saturation (40) at the time when denitrification in these zones may be particularly active (36). Although it is possible that this NO₃⁻-depleted water is later exported to the stream (15), these data show concentrations of NO₃⁻ in groundwater equal to or higher than those of streamwater, suggesting that this was not the case at the time of sampling. If these episodes of fragmented denitrification have increased over time, for example, with the increases in summer temperature and precipitation that have been observed at this site (10, 43), then denitrification may have significantly contributed both to the amount of “missing N” in the ecosystem N balance and to the observed changes in this balance over time.

Methods

Site Description.

This study focused on watershed 3 (WS3), a 41.2-ha hydrologic reference watershed at the HBEF (43°56’N, 71°45’W). Temperatures average −9 °C in January and 18 °C in July, and annual precipitation averages 1,400 mm, ∼70% as rain (44). WS3 is steep and south facing, with an elevation range of 537–732 m. The HBEF is covered by second-growth northern hardwoods naturally regenerated after harvesting between 1910 and 1917. WS3 is underlain by mica schist bedrock of the Silurian Rangeley Formation, and covered by Wisconsinan glacial tills. Spodosols of sandy loam to loamy sand texture comprise ∼80% of catchment soils, and Inceptisols and Histosols make up the rest (45). The C horizon occurs at ∼70 cm depth and has variable although generally lower hydraulic conductivity compared with the overlying B horizon (40). A shallow groundwater system with a transient saturated zone develops within the solum throughout the catchment (45). A more consistent saturated zone is present in the near-stream region that is typically hydrologically connected to surface water in perennial stream reaches and is at or near the surface beneath ephemeral and intermittent stream reaches (40). WS3 is drained by Paradise Brook, a second-order perennial stream fed by several ephemeral and intermittent tributaries, which together comprise 79% of the stream length within the catchment (45). The quickflow response of Paradise Brook to storm inputs is highly nonlinear, with a large and rapid runoff response observed when thresholds in soil moisture content and storm event size are exceeded (34).

Sample Collection and Analysis.

Sampling encompassed 11 locations along Paradise Brook and 8 locations on tributaries, along with 1 seep and 9 shallow groundwater wells selected from >30 wells installed previously in WS3 (34, 40, 41), and chosen for their tendency to provide water during midsummer (40) and ease of repeated sampling. The wells included three wells in or within 2 m of the perennial channel of Paradise Brook and forming a transect across it (R12 on the east, R13 in-stream, and R14 on the west). The other six wells were ≥4 m away and upgradient from surface streamflow during July 2011, and included two wells located within a seasonally dry side-stream channel (JD29, JD30), one well ∼4 m from the perennial stream and generally upgradient from it (JD05), and three wells that form an upslope transect ∼3–29 m from a seasonally dry tributary (JD17, JD18, JD19). Well depth ranged from 30 cm (JD30) to 115 cm (JD05), with most wells accessing water from the lower mineral horizon. Three wells (JD05, JD17 and JD18), the seep, and seven sites on Paradise Brook and its tributaries were sampled on six dates on a near-daily basis (3, 4, 5, 7, 8, and 12 July 2011). The three near-stream wells were sampled up to nine times at 20–40 min intervals on 11 July 2011, along with concurrent sampling from Paradise Brook 0.5 m upstream of the wells. A mix of precipitation and throughfall was collected from a small clearing at the southern WS3 boundary using a rinsed 10-L plastic collector. Stream samples were collected with a cleaned high-density polyethylene collector. Well samples were collected using a peristaltic pump, after purging. Streamflow, rain gauge, and air temperature data were provided by the US Forest Service (44). Streamflow was measured using a sharp-crested V-notch weir at the watershed outlet, and rain was gauged by standard and weighing-recording.

Solution concentrations of NO₃⁻, NO₂⁻, and NH₄⁺ were measured using ion chromatography (Dionex ICS-2000; Dionex Corp.); all NO₂⁻ concentrations were below the detection limit (0.1 μM). Total dissolved nitrogen (TDN) and dissolved organic carbon (DOC) were measured by combustion with a Shimadzu TOC-V_CPN and TNM-1 chemiluminescent detector (Kyoto, Japan). DON concentration was calculated by difference (DON = TDN – NO₃⁻ – NH₄⁺). Samples for isotope analysis were frozen at −20 °C and later prepared at Cornell University using the denitrifier method (46, 47) to produce N₂O gas, which was sent to the Stable Isotope Facility, UC Davis, CA, for analysis of δ¹⁸O and δ¹⁵N, with a precision of ±0.3‰ for both isotopes. Three rainfall, eight stream, and nine groundwater samples spanning a range of sites and dates were selected for measurement of δ¹⁸O_H2O at the Stable Isotope Laboratory, School of Environmental Sciences, University of East Anglia, on a Picarro L1102-i with a precision of ±0.2‰.

Isotopic End-Member Determination.

Expected isotopic values for NO₃⁻ source end-members were determined through a combination of field- and literature-based estimates. Rainfall NO₃⁻ was highly enriched in ¹⁸O and slightly depleted in ¹⁵N (Table 1 and Fig. 3), resembling previous measurements at the HBEF (10, 17). Nitrate produced by microbial nitrification reflects the δ¹⁵N of the NH₄⁺ that these autotrophic bacteria consume, as well as any fractionation that occurs during this process. Ammonium can be supplied both by atmospheric deposition and by the much larger flux of mineralization of organic N by heterotrophic microbes (7, 8). We measured δ¹⁵N of soil samples collected from nine soil pits in WS3 (37) using a Finnigan MAT DeltaPlus isotope ratio mass spectrometer (Thermo Finnigan) at the Cornell Stable Isotope Lab. These δ¹⁵N values averaged 1.2 ± 0.4‰ in the surface organic horizons (Oi/Oe) and increased to 5.2 ± 1.1‰ in the Oa horizon and 6.0 ± 1.1‰ in Bh and Bhs horizons at 10–65 cm depth, similar to prior measurements in WS3 (48). Mineralization causes negligible ¹⁵N fractionation (14, 49) and so δ¹⁵N_NH4 from mineralization of this soil organic N should also have this range of ¹⁵N values (0.5–7.5‰). Isotopic fractionation during nitrification should be negligible when NH₄⁺ supply rate limits the rate of nitrification, but fractionation can occur when nitrification is slow or incomplete relative to the NH₄⁺ supply, producing δ¹⁵N_NO3 values ∼2–20‰ lighter than those of the NH₄⁺ substrate (14, 20, 49). At WS3, net nitrification consumes a highly variable fraction of mineralized N, but this fraction generally increases with soil depth (37). Together, these measurements indicate that microbially produced δ¹⁵N_NO3 could approach 7.5‰ in mineral soils or could be considerably lighter, particularly in surface soils, assumed here to range to −10‰ (14).

The δ¹⁸O_NO3 value for NO₃⁻ produced by microbial nitrification with no kinetic isotope effects or oxygen exchange should reflect nitrifier acquisition of two oxygen atoms from water and one from atmospheric O₂ (δ¹⁸O_{NO3-nitrification} = 2/3 δ¹⁸O_H2O + 1/3 δ¹⁸O_O2) (14, 26, 27). The δ¹⁸O_H2O composition of streamwater and shallow groundwater during the sampling period was −9.2 ± 0.2‰ (n = 17) and the established value for δ¹⁸O_O2 of air is 23.5‰, yielding a value of δ¹⁸O_NO3 of 1.7‰ ± 0.1‰. However, kinetic isotope effects controlled by parameters including pH and temperature can occur during nitrite oxidation and during incorporation water, affecting the final oxygen isotope composition of the nitrite (16, 28 ⇓–30). This process is also affected by the residence time of nitrite, which is highly susceptible to microbially mediated oxygen exchange with water yielding markedly lighter δ¹⁸O_NO3 than expected otherwise (28 ⇓–30).

Acknowledgments

Thanks to Kristin Waeber, Maggie Burns, Maggie Zimmer, Colin Fuss, J. P. Gannon, Jennifer Morse, and Guinevere Fredriksen for field and lab advice and assistance; Amey Bailey and John Campbell for meteorological information; and Linda Pardo, J. P. Gannon, and John Campbell for valuable comments. The Hubbard Brook Experimental Forest is operated and maintained by the Northern Research Station, US Department of Agriculture Forest Service, Newtown Square, PA. Financial support for this project was provided by the US National Science Foundation Ecosystem Studies (DEB-0919131), Long-term Ecological Research (DEB-1114804), and Hydrologic Sciences (EAR 1014507) programs.

Footnotes

↵¹To whom correspondence may be addressed. Email: s.wexler1{at}uea.ac.uk or clg33{at}cornell.edu.

Author contributions: S.K.W., C.L.G., K.J.M., S.W.B., and P.M.G. designed research; S.K.W. performed research; S.K.W. and C.L.G. analyzed data; and S.K.W., C.L.G., K.J.M., S.W.B., and P.M.G. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1404321111/-/DCSupplemental.

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OpenUrl CrossRef
↵
1. Goodale CL,
2. Aber JD,
3. Vitousek PM
(2003) An unexpected nitrate decline in New Hampshire streams. Ecosystems 6(1):75–86
.
OpenUrl CrossRef
↵
1. Durka W,
2. Schulze E-D,
3. Gebauer G,
4. Voerkelius S
(1994) Effects of forest decline on uptake and leaching of deposited nitrate determined from ¹⁵N and ¹⁸O measurements. Nature 372:765–767
.
OpenUrl CrossRef
↵
1. Lajtha K,
2. Michener R
1. Kendall C,
2. Elliott EM,
3. Wankel SD
(2007) Tracing anthropogenic inputs of nitrogen to ecosystems. Stable Isotopes in Ecology and Environmental Science, eds Lajtha K, Michener R (Blackwell, Oxford), 2nd Ed, pp 375–449
.
↵
1. Osaka K,
2. Ohte N,
3. Koba K,
4. Yoshimizu C,
5. Katsuyama M,
6. Tani M,
7. Tayasu I,
8. Nagata T
(2010) Hydrological influences on spatiotemporal variations in δ¹⁵N and δ¹⁸O of nitrate in a forested headwater in Japan: Denitrification plays a critical role in groundwater. J Geophys Res 115:G02021
.
OpenUrl
↵
1. Fang Y, et al.
(2012) Low δ¹⁸O values of nitrate produced from nitrification in temperate forest soils. Environ Sci Technol 46(16):8723–8730
.
OpenUrl CrossRef PubMed
↵
1. Pardo LH,
2. Kendall C,
3. Pett-Ridge J,
4. Chang CCY
(2004) Evaluating the source of streamwater nitrate using δ¹⁵N and δ¹⁸O in nitrate in two watersheds in New Hampshire, USA. Hydrol Processes 18:2699–2712
.
OpenUrl CrossRef
↵
1. Richey JS,
2. McDowell WH,
3. Likens GE
(1985) Nitrogen transformations in a small mountain stream. Hydrobiologia 124:129–139
.
OpenUrl CrossRef
↵
1. Curtis CJ,
2. Evans CD,
3. Goodale CL,
4. Heaton THE
(2011) What have stable isotope studies revealed about the nature and mechanics of N saturation and nitrate leaching from semi-natural catchments? Ecosystems 14:1021–1037
.
OpenUrl CrossRef
↵
1. Mariotti A, et al.
(1981) Experimental determination of nitrogen kinetic isotope fractionation: Some principles; illustration for the denitrification and nitrification processes. Plant Soil 62:413–430
.
OpenUrl CrossRef
↵
1. Deutsch B,
2. Liskow I,
3. Kahle P,
4. Voss M
(2005) Variations of the δ¹⁵N and δ¹⁸O values of nitrate in drainage water of two fertilized fields in Mecklenburg-Vorpommern (Germany). Aquat Sci 67:156–165
.
OpenUrl CrossRef
↵
1. Granger J,
2. Sigman DM,
3. Lehmann MF,
4. Tortell PD
(2008) Nitrogen and oxygen isotope fractionation during dissimilatory nitrate reduction by denitrifying bacteria. Limnol Oceanogr 53:2533–2545
.
OpenUrl CrossRef
↵
1. Wexler SK,
2. Hiscock KM,
3. Dennis PF
(2011) Catchment-scale quantification of hyporheic denitrification using an isotopic and solute flux approach. Environ Sci Technol 45(9):3967–3973
.
OpenUrl CrossRef PubMed
↵
1. Lohse KA,
2. Sanderman J,
3. Amundson R
(2013) Identifying sources and processes influencing nitrogen export to a small stream using dual isotopes of nitrate. Water Resour Res 49:5715–5731
.
OpenUrl CrossRef
↵
1. Kulkarni AJ,
2. Groffman PM,
3. Yavitt JB,
4. Goodale CL
(2014) Complex controls of denitrification at ecosystem, landscape and regional scales in northern hardwood forests. Ecol Modell, in press
.
↵
1. Andersson KK,
2. Hooper AB
(1983) O₂ and H₂O are each the source of one O in NO₂ produced from NH₃ by Nitrosomonas: ¹⁵N-NMR evidence. FEBS Lett 164:236–240
.
OpenUrl CrossRef
↵
1. Kumar S,
2. Nicholas DJD,
3. Williams EH
(1983) Definitive ¹⁵N NMR evidence that water serves as a source of ‘O’ during nitrite oxidation by Nitrobacter agilis. FEBS Lett 152(1):71–74
.
OpenUrl CrossRef PubMed
↵
1. Casciotti KL,
2. McIlvin M,
3. Buchwald C
(2010) Oxygen isotopic exchange and fractionation during bacterial ammonia oxidation. Limnol Oceanogr 55:753–762
.
OpenUrl CrossRef
↵
1. Snider DM,
2. Spoelstra J,
3. Schiff SL,
4. Venkiteswaran JJ
(2010) Stable oxygen isotope ratios of nitrate produced from nitrification: ¹⁸O-labeled water incubations of agricultural and temperate forest soils. Environ Sci Technol 44(14):5358–5364
.
OpenUrl CrossRef PubMed
↵
1. Buchwald C,
2. Casciotti KL
(2010) Oxygen isotopic fractionation and exchange during bacterial nitrite oxidation. Limnol Oceanogr 55:1064–1074
.
OpenUrl CrossRef
↵
1. Hall RO Jr,
2. Bernhardt ES,
3. Likens GE
(2002) Relating nutrient uptake with transient storage in forested mountain streams. Limnol Oceanogr 47(1):255–265
.
OpenUrl CrossRef
↵
1. Bernhardt ES,
2. Hall RO Jr,
3. Likens GE
(2002) Whole-system estimates of nitrification and nitrate uptake in streams of the Hubbard Brook Experimental Forest. Ecosystems 5:419–430
.
OpenUrl CrossRef
↵
1. Peterson BJ, et al.
(2001) Control of nitrogen export from watersheds by headwater streams. Science 292(5514):86–90
.
OpenUrl Abstract/FREE Full Text
↵
1. Detty JM,
2. McGuire KJ
(2010) Threshold changes in storm runoff generation at a till mantled headwater catchment. Water Resour Res 46(7):W07525
.
OpenUrl
↵
1. Houlton BZ,
2. Bai E
(2009) Imprint of denitrifying bacteria on the global terrestrial biosphere. Proc Natl Acad Sci USA 106(51):21713–21716
.
OpenUrl Abstract/FREE Full Text
↵
1. Werner SF,
2. Driscoll CT,
3. Groffman PM,
4. Yavitt JB
(2011) Landscape patterns of soil oxygen and atmospheric gases in a northern hardwood forest landscape. Biogeosci Discuss 8:10859–10893
.
OpenUrl CrossRef
↵
1. Morse JL, et al.
(2014) Searching for biogeochemical hotspots in three dimensions: soil C and N cycling in hydropedologic settings in a northern hardwood forest. J Geophys Res 119(8):1596−1607
.
↵
1. McClain ME, et al.
(2003) Biogeochemical hot spots and hot moments at the interface of terrestrial and aquatic ecosystems. Ecosystems 6:301–312
.
OpenUrl CrossRef
↵
1. Dittman JA,
2. Driscoll CT,
3. Groffman PM,
4. Fahey TJ
(2007) Dynamics of nitrogen and dissolved organic carbon at the Hubbard brook experimental forest. Ecology 88(5):1153–1166
.
OpenUrl CrossRef PubMed
↵
1. Detty JM,
2. McGuire KJ
(2010) Topographical controls on shallow groundwater dynamics: Implications of hydrological connectivity between hillslopes and riparian zones in a till mantled catchment. Hydrol Processes 24:2222–2236
.
OpenUrl CrossRef
↵
1. Zimmer MA,
2. Bailey SW,
3. McGuire KJ,
4. Bullen TD
(2012) Fine scale variations of surface water chemistry in an ephemeral to perennial drainage network. Hydrol Processes 57(24):3438–3451
.
OpenUrl
↵
1. Ocampo CJ,
2. Sivapalan M,
3. Oldham C
(2006) Hydrological connectivity of upland-riparian zones in agricultural catchments: Implications for runoff generation and nitrate transport. J Hydrol 331:643–658
.
OpenUrl CrossRef
↵
1. Campbell JL,
2. Driscoll CT,
3. Pourmokhtarian A,
4. Hayhoe K
(2011) Streamflow responses to past and projected future changes in climate at the Hubbard Brook Experimental Forest, New Hampshire, USA. Water Resour Res 47(2):W02514
.
OpenUrl
↵
1. Bailey AS,
2. Hornbeck JW,
3. Campbell JL,
4. Eagar C
(2003) Hydrometeorological Database for Hubbard Brook Experimental Forest: 1955−2000 (US Dep Agriculture, Washington, DC)
.
↵
1. Bailey SW,
2. Brousseau PA,
3. McGuire KM,
4. Ross DS
(2014) Influence of landscape position and transient water table on soil development and carbon distribution in a steep, headwater catchment. Geoderma 226-227:279–289
.
OpenUrl
↵
1. Sigman DM, et al.
(2001) A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater. Anal Chem 73(17):4145–4153
.
OpenUrl CrossRef PubMed
↵
1. Casciotti KL,
2. Sigman DM,
3. Hastings MG,
4. Böhlke JK,
5. Hilkert A
(2002) Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method. Anal Chem 74(19):4905–4912
.
OpenUrl CrossRef PubMed
↵
1. Pardo LH,
2. Hemond HF,
3. Montoya JP,
4. Fahey TJ,
5. Siccama TG
(2007) Natural abundance ¹⁵N in soil and litter across a nitrate-output gradient in New Hampshire. For Ecol Manage 251:217–230
.
OpenUrl CrossRef
↵
1. Högberg P
(1997) Tansley Review No. 95: ¹⁵N natural abundance in soil-plant systems. New Phytol 137:179–203
.
OpenUrl CrossRef

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Environmental Sciences

Proceedings of the National Academy of Sciences: 111 (46)

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[1] ↵
Aber JD, et al.
(2003) Is nitrogen deposition altering the nitrogen status of northeastern forests? Bioscience 53:375–389
.
OpenUrl Abstract/FREE Full Text

[2] Aber JD, et al.

[3] ↵
McDonald JA, et al.
(2002) Nitrogen input together with ecosystem nitrogen enrichment predict nitrate leaching from European forests. Global Change Biol 8:1028–1033
.
OpenUrl CrossRef

[4] McDonald JA, et al.

[5] ↵
Groffman PM, et al.
(2006) Methods for measuring denitrification: Diverse approaches to a difficult problem. Ecol Appl 16(6):2091–2122
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OpenUrl CrossRef PubMed

[6] Groffman PM, et al.

[7] ↵
Kulkarni MV,
Burgin AJ,
Groffman PM,
Yavitt JB
(2014) Direct flux and ¹⁵N tracer methods for measuring denitrification in forest soils. Biogeochemistry 117:359–373
.
OpenUrl CrossRef

[8] Kulkarni MV,

[9] Burgin AJ,

[10] Groffman PM,

[11] Yavitt JB

[12] ↵
Melillo JM,
Aber JD,
Steudler PA,
Schimel JP
(1983) Denitrification potentials in a successional sequence of northern hardwood forest stands. Ecol Bull 35:217–228
.
OpenUrl

[13] Melillo JM,

[14] Aber JD,

[15] Steudler PA,

[16] Schimel JP

[17] ↵
Bowden WB,
Bormann FH
(1986) Transport and loss of nitrous oxide in soil water after forest clear-cutting. Science 233(4766):867–869
.
OpenUrl Abstract/FREE Full Text

[18] Bowden WB,

[19] Bormann FH

[20] ↵
Bormann FH,
Likens GE,
Melillo JM
(1977) Nitrogen budget for an aggrading northern hardwood forest ecosystem. Science 196(4293):981–983
.
OpenUrl Abstract/FREE Full Text

[21] Bormann FH,

[22] Likens GE,

[23] Melillo JM

[24] ↵
Likens GE,
Bormann FH
(1995) Biogeochemistry of a Forested Ecosystem (Springer, New York)
.

[25] Likens GE,

[26] Bormann FH

[27] ↵
Bernhardt ES, et al.
(2005) Can’t see the forest for the stream? In-stream processing and terrestrial nitrogen exports. Bioscience 55:219–230
.
OpenUrl Abstract/FREE Full Text

[28] Bernhardt ES, et al.

[29] ↵
Bernal S,
Hedin LO,
Likens GE,
Gerber S,
Buso DC
(2012) Complex response of the forest nitrogen cycle to climate change. Proc Natl Acad Sci USA 109(9):3406–3411
.
OpenUrl Abstract/FREE Full Text

[30] Bernal S,

[31] Hedin LO,

[32] Likens GE,

[33] Gerber S,

[34] Buso DC

[35] ↵
Fahey TJ, et al.
(2005) The biogeochemistry of carbon at Hubbard Brook. Biogeochemistry 75:109–176
.
OpenUrl CrossRef

[36] Fahey TJ, et al.

[37] ↵
Goodale CL,
Aber JD,
Vitousek PM
(2003) An unexpected nitrate decline in New Hampshire streams. Ecosystems 6(1):75–86
.
OpenUrl CrossRef

[38] Goodale CL,

[39] Aber JD,

[40] Vitousek PM

[41] ↵
Durka W,
Schulze E-D,
Gebauer G,
Voerkelius S
(1994) Effects of forest decline on uptake and leaching of deposited nitrate determined from ¹⁵N and ¹⁸O measurements. Nature 372:765–767
.
OpenUrl CrossRef

[42] Durka W,

[43] Schulze E-D,

[44] Gebauer G,

[45] Voerkelius S

[46] ↵
Lajtha K,
Michener R
Kendall C,
Elliott EM,
Wankel SD
(2007) Tracing anthropogenic inputs of nitrogen to ecosystems. Stable Isotopes in Ecology and Environmental Science, eds Lajtha K, Michener R (Blackwell, Oxford), 2nd Ed, pp 375–449
.

[47] Lajtha K,

[48] Michener R

[49] Kendall C,

[50] Elliott EM,

[51] Wankel SD

[52] ↵
Osaka K,
Ohte N,
Koba K,
Yoshimizu C,
Katsuyama M,
Tani M,
Tayasu I,
Nagata T
(2010) Hydrological influences on spatiotemporal variations in δ¹⁵N and δ¹⁸O of nitrate in a forested headwater in Japan: Denitrification plays a critical role in groundwater. J Geophys Res 115:G02021
.
OpenUrl

[53] Osaka K,

[54] Ohte N,

[55] Koba K,

[56] Yoshimizu C,

[57] Katsuyama M,

[58] Tani M,

[59] Tayasu I,

[60] Nagata T

[61] ↵
Fang Y, et al.
(2012) Low δ¹⁸O values of nitrate produced from nitrification in temperate forest soils. Environ Sci Technol 46(16):8723–8730
.
OpenUrl CrossRef PubMed

[62] Fang Y, et al.

[63] ↵
Pardo LH,
Kendall C,
Pett-Ridge J,
Chang CCY
(2004) Evaluating the source of streamwater nitrate using δ¹⁵N and δ¹⁸O in nitrate in two watersheds in New Hampshire, USA. Hydrol Processes 18:2699–2712
.
OpenUrl CrossRef

[64] Pardo LH,

[65] Kendall C,

[66] Pett-Ridge J,

[67] Chang CCY

[68] ↵
Richey JS,
McDowell WH,
Likens GE
(1985) Nitrogen transformations in a small mountain stream. Hydrobiologia 124:129–139
.
OpenUrl CrossRef

[69] Richey JS,

[70] McDowell WH,

[71] Likens GE

[72] ↵
Curtis CJ,
Evans CD,
Goodale CL,
Heaton THE
(2011) What have stable isotope studies revealed about the nature and mechanics of N saturation and nitrate leaching from semi-natural catchments? Ecosystems 14:1021–1037
.
OpenUrl CrossRef

[73] Curtis CJ,

[74] Evans CD,

[75] Goodale CL,

[76] Heaton THE

[77] ↵
Mariotti A, et al.
(1981) Experimental determination of nitrogen kinetic isotope fractionation: Some principles; illustration for the denitrification and nitrification processes. Plant Soil 62:413–430
.
OpenUrl CrossRef

[78] Mariotti A, et al.

[79] ↵
Deutsch B,
Liskow I,
Kahle P,
Voss M
(2005) Variations of the δ¹⁵N and δ¹⁸O values of nitrate in drainage water of two fertilized fields in Mecklenburg-Vorpommern (Germany). Aquat Sci 67:156–165
.
OpenUrl CrossRef

[80] Deutsch B,

[81] Liskow I,

[82] Kahle P,

[83] Voss M

[84] ↵
Granger J,
Sigman DM,
Lehmann MF,
Tortell PD
(2008) Nitrogen and oxygen isotope fractionation during dissimilatory nitrate reduction by denitrifying bacteria. Limnol Oceanogr 53:2533–2545
.
OpenUrl CrossRef

[85] Granger J,

[86] Sigman DM,

[87] Lehmann MF,

[88] Tortell PD

[89] ↵
Wexler SK,
Hiscock KM,
Dennis PF
(2011) Catchment-scale quantification of hyporheic denitrification using an isotopic and solute flux approach. Environ Sci Technol 45(9):3967–3973
.
OpenUrl CrossRef PubMed

[90] Wexler SK,

[91] Hiscock KM,

[92] Dennis PF

[93] ↵
Lohse KA,
Sanderman J,
Amundson R
(2013) Identifying sources and processes influencing nitrogen export to a small stream using dual isotopes of nitrate. Water Resour Res 49:5715–5731
.
OpenUrl CrossRef

[94] Lohse KA,

[95] Sanderman J,

[96] Amundson R

[97] ↵
Kulkarni AJ,
Groffman PM,
Yavitt JB,
Goodale CL
(2014) Complex controls of denitrification at ecosystem, landscape and regional scales in northern hardwood forests. Ecol Modell, in press
.

[98] Kulkarni AJ,

[99] Groffman PM,

[100] Yavitt JB,

[101] Goodale CL

[102] ↵
Andersson KK,
Hooper AB
(1983) O₂ and H₂O are each the source of one O in NO₂ produced from NH₃ by Nitrosomonas: ¹⁵N-NMR evidence. FEBS Lett 164:236–240
.
OpenUrl CrossRef

[103] Andersson KK,

[104] Hooper AB

[105] ↵
Kumar S,
Nicholas DJD,
Williams EH
(1983) Definitive ¹⁵N NMR evidence that water serves as a source of ‘O’ during nitrite oxidation by Nitrobacter agilis. FEBS Lett 152(1):71–74
.
OpenUrl CrossRef PubMed

[106] Kumar S,

[107] Nicholas DJD,

[108] Williams EH

[109] ↵
Casciotti KL,
McIlvin M,
Buchwald C
(2010) Oxygen isotopic exchange and fractionation during bacterial ammonia oxidation. Limnol Oceanogr 55:753–762
.
OpenUrl CrossRef

[110] Casciotti KL,

[111] McIlvin M,

[112] Buchwald C

[113] ↵
Snider DM,
Spoelstra J,
Schiff SL,
Venkiteswaran JJ
(2010) Stable oxygen isotope ratios of nitrate produced from nitrification: ¹⁸O-labeled water incubations of agricultural and temperate forest soils. Environ Sci Technol 44(14):5358–5364
.
OpenUrl CrossRef PubMed

[114] Snider DM,

[115] Spoelstra J,

[116] Schiff SL,

[117] Venkiteswaran JJ

[118] ↵
Buchwald C,
Casciotti KL
(2010) Oxygen isotopic fractionation and exchange during bacterial nitrite oxidation. Limnol Oceanogr 55:1064–1074
.
OpenUrl CrossRef

[119] Buchwald C,

[120] Casciotti KL

[121] ↵
Hall RO Jr,
Bernhardt ES,
Likens GE
(2002) Relating nutrient uptake with transient storage in forested mountain streams. Limnol Oceanogr 47(1):255–265
.
OpenUrl CrossRef

[122] Hall RO Jr,

[123] Bernhardt ES,

[124] Likens GE

[125] ↵
Bernhardt ES,
Hall RO Jr,
Likens GE
(2002) Whole-system estimates of nitrification and nitrate uptake in streams of the Hubbard Brook Experimental Forest. Ecosystems 5:419–430
.
OpenUrl CrossRef

[126] Bernhardt ES,

[127] Hall RO Jr,

[128] Likens GE

[129] ↵
Peterson BJ, et al.
(2001) Control of nitrogen export from watersheds by headwater streams. Science 292(5514):86–90
.
OpenUrl Abstract/FREE Full Text

[130] Peterson BJ, et al.

[131] ↵
Detty JM,
McGuire KJ
(2010) Threshold changes in storm runoff generation at a till mantled headwater catchment. Water Resour Res 46(7):W07525
.
OpenUrl

[132] Detty JM,

[133] McGuire KJ

[134] ↵
Houlton BZ,
Bai E
(2009) Imprint of denitrifying bacteria on the global terrestrial biosphere. Proc Natl Acad Sci USA 106(51):21713–21716
.
OpenUrl Abstract/FREE Full Text

[135] Houlton BZ,

[136] Bai E

[137] ↵
Werner SF,
Driscoll CT,
Groffman PM,
Yavitt JB
(2011) Landscape patterns of soil oxygen and atmospheric gases in a northern hardwood forest landscape. Biogeosci Discuss 8:10859–10893
.
OpenUrl CrossRef

[138] Werner SF,

[139] Driscoll CT,

[140] Groffman PM,

[141] Yavitt JB

[142] ↵
Morse JL, et al.
(2014) Searching for biogeochemical hotspots in three dimensions: soil C and N cycling in hydropedologic settings in a northern hardwood forest. J Geophys Res 119(8):1596−1607
.

[143] Morse JL, et al.

[144] ↵
McClain ME, et al.
(2003) Biogeochemical hot spots and hot moments at the interface of terrestrial and aquatic ecosystems. Ecosystems 6:301–312
.
OpenUrl CrossRef

[145] McClain ME, et al.

[146] ↵
Dittman JA,
Driscoll CT,
Groffman PM,
Fahey TJ
(2007) Dynamics of nitrogen and dissolved organic carbon at the Hubbard brook experimental forest. Ecology 88(5):1153–1166
.
OpenUrl CrossRef PubMed

[147] Dittman JA,

[148] Driscoll CT,

[149] Groffman PM,

[150] Fahey TJ

[151] ↵
Detty JM,
McGuire KJ
(2010) Topographical controls on shallow groundwater dynamics: Implications of hydrological connectivity between hillslopes and riparian zones in a till mantled catchment. Hydrol Processes 24:2222–2236
.
OpenUrl CrossRef

[152] Detty JM,

[153] McGuire KJ

[154] ↵
Zimmer MA,
Bailey SW,
McGuire KJ,
Bullen TD
(2012) Fine scale variations of surface water chemistry in an ephemeral to perennial drainage network. Hydrol Processes 57(24):3438–3451
.
OpenUrl

[155] Zimmer MA,

[156] Bailey SW,

[157] McGuire KJ,

[158] Bullen TD

[159] ↵
Ocampo CJ,
Sivapalan M,
Oldham C
(2006) Hydrological connectivity of upland-riparian zones in agricultural catchments: Implications for runoff generation and nitrate transport. J Hydrol 331:643–658
.
OpenUrl CrossRef

[160] Ocampo CJ,

[161] Sivapalan M,

[162] Oldham C

[163] ↵
Campbell JL,
Driscoll CT,
Pourmokhtarian A,
Hayhoe K
(2011) Streamflow responses to past and projected future changes in climate at the Hubbard Brook Experimental Forest, New Hampshire, USA. Water Resour Res 47(2):W02514
.
OpenUrl

[164] Campbell JL,

[165] Driscoll CT,

[166] Pourmokhtarian A,

[167] Hayhoe K

[168] ↵
Bailey AS,
Hornbeck JW,
Campbell JL,
Eagar C
(2003) Hydrometeorological Database for Hubbard Brook Experimental Forest: 1955−2000 (US Dep Agriculture, Washington, DC)
.

[169] Bailey AS,

[170] Hornbeck JW,

[171] Campbell JL,

[172] Eagar C

[173] ↵
Bailey SW,
Brousseau PA,
McGuire KM,
Ross DS
(2014) Influence of landscape position and transient water table on soil development and carbon distribution in a steep, headwater catchment. Geoderma 226-227:279–289
.
OpenUrl

[174] Bailey SW,

[175] Brousseau PA,

[176] McGuire KM,

[177] Ross DS

[178] ↵
Sigman DM, et al.
(2001) A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater. Anal Chem 73(17):4145–4153
.
OpenUrl CrossRef PubMed

[179] Sigman DM, et al.

[180] ↵
Casciotti KL,
Sigman DM,
Hastings MG,
Böhlke JK,
Hilkert A
(2002) Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method. Anal Chem 74(19):4905–4912
.
OpenUrl CrossRef PubMed

[181] Casciotti KL,

[182] Sigman DM,

[183] Hastings MG,

[184] Böhlke JK,

[185] Hilkert A

[186] ↵
Pardo LH,
Hemond HF,
Montoya JP,
Fahey TJ,
Siccama TG
(2007) Natural abundance ¹⁵N in soil and litter across a nitrate-output gradient in New Hampshire. For Ecol Manage 251:217–230
.
OpenUrl CrossRef

[187] Pardo LH,

[188] Hemond HF,

[189] Montoya JP,

[190] Fahey TJ,

[191] Siccama TG

[192] ↵
Högberg P
(1997) Tansley Review No. 95: ¹⁵N natural abundance in soil-plant systems. New Phytol 137:179–203
.
OpenUrl CrossRef

[193] Högberg P

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Isotopic signals of summer denitrification in a northern hardwood forested catchment

Significance

Abstract

What Is the Source of Streamwater NO₃⁻?

Groundwater Denitrification Is Consuming Soil NO₃⁻

Methods

Site Description.

Sample Collection and Analysis.

Isotopic End-Member Determination.

Acknowledgments

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Significance

Abstract

What Is the Source of Streamwater NO3−?

Groundwater Denitrification Is Consuming Soil NO3−

Methods

Site Description.

Sample Collection and Analysis.

Isotopic End-Member Determination.

Acknowledgments

Footnotes

References

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What Is the Source of Streamwater NO₃⁻?

Groundwater Denitrification Is Consuming Soil NO₃⁻