Table of Contents

Biological Sciences

• Applied Biological Sciences

  • Open Access

    Anticancer drug nanomicelles formed by self-assembling amphiphilic dendrimer to combat cancer drug resistance

    Tuo Wei, Chao Chen, Juan Liu, Cheng Liu, Paola Posocco, Xiaoxuan Liu, Qiang Cheng, Shuaidong Huo, Zicai Liang, Maurizio Fermeglia, Sabrina Pricl, Xing-Jie Liang, Palma Rocchi, and Ling Peng

    PNAS March 10, 2015 112 (10) 2978-2983; first published February 23, 2015. https://doi.org/10.1073/pnas.1418494112

• Biochemistry

  • Open Access

    Rational design of a split-Cas9 enzyme complex

    Addison V. Wright, Samuel H. Sternberg, David W. Taylor, Brett T. Staahl, Jorge A. Bardales, Jack E. Kornfeld, and Jennifer A. Doudna

    PNAS March 10, 2015 112 (10) 2984-2989; first published February 23, 2015. https://doi.org/10.1073/pnas.1501698112
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    Selective molecular transport through the protein shell of a bacterial microcompartment organelle

    Chiranjit Chowdhury, Sunny Chun, Allan Pang, Michael R. Sawaya, Sharmistha Sinha, Todd O. Yeates, and Thomas A. Bobik

    PNAS March 10, 2015 112 (10) 2990-2995; first published February 23, 2015. https://doi.org/10.1073/pnas.1423672112

• Biophysics and Computational Biology

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    On the principle of ion selectivity in Na⁺/H⁺-coupled membrane proteins: Experimental and theoretical studies of an ATP synthase rotor

    Vanessa Leone, Denys Pogoryelov, Thomas Meier, and José D. Faraldo-Gómez

    PNAS March 10, 2015 112 (10) E1057-E1066; first published February 23, 2015. https://doi.org/10.1073/pnas.1421202112
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    Tunable assembly of amyloid-forming peptides into nanosheets as a retrovirus carrier

    Bin Dai, Dan Li, Wenhui Xi, Fang Luo, Xiang Zhang, Man Zou, Mi Cao, Jun Hu, Wenyuan Wang, Guanghong Wei, Yi Zhang, and Cong Liu

    PNAS March 10, 2015 112 (10) 2996-3001; first published February 23, 2015. https://doi.org/10.1073/pnas.1416690112

• Cell Biology

  • Open Access

    l-cysteine reversibly inhibits glucose-induced biphasic insulin secretion and ATP production by inactivating PKM2

    Daiki Nakatsu, Yuta Horiuchi, Fumi Kano, Yoshiyuki Noguchi, Taichi Sugawara, Iseki Takamoto, Naoto Kubota, Takashi Kadowaki, and Masayuki Murata

    PNAS March 10, 2015 112 (10) E1067-E1076; first published February 23, 2015. https://doi.org/10.1073/pnas.1417197112
  • Open Access

    Morphogenesis checkpoint kinase Swe1 is the executor of lipolysis-dependent cell-cycle progression

    Neha Chauhan, Myriam Visram, Alvaro Cristobal-Sarramian, Florian Sarkleti, and Sepp D. Kohlwein

    PNAS March 10, 2015 112 (10) E1077-E1085; first published February 23, 2015. https://doi.org/10.1073/pnas.1423175112
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    Rapid labeling of intracellular His-tagged proteins in living cells

    Yau-Tsz Lai, Yuen-Yan Chang, Ligang Hu, Ya Yang, Ailun Chao, Zhi-Yan Du, Julian A. Tanner, Mee-Len Chye, Chengmin Qian, Kwan-Ming Ng, Hongyan Li, and Hongzhe Sun

    PNAS March 10, 2015 112 (10) 2948-2953; first published February 23, 2015. https://doi.org/10.1073/pnas.1419598112
  • Open Access

    Multicolor CRISPR labeling of chromosomal loci in human cells

    Hanhui Ma, Ardalan Naseri, Pablo Reyes-Gutierrez, Scot A. Wolfe, Shaojie Zhang, and Thoru Pederson

    PNAS March 10, 2015 112 (10) 3002-3007; first published February 23, 2015. https://doi.org/10.1073/pnas.1420024112

• Developmental Biology

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    Histone titration against the genome sets the DNA-to-cytoplasm threshold for the Xenopus midblastula transition

    Amanda A. Amodeo, David Jukam, Aaron F. Straight, and Jan M. Skotheim

    PNAS March 10, 2015 112 (10) E1086-E1095; first published February 23, 2015. https://doi.org/10.1073/pnas.1413990112

• Ecology

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    Predator diversity, intraguild predation, and indirect effects drive parasite transmission

    Jason R. Rohr, David J. Civitello, Patrick W. Crumrine, Neal T. Halstead, Andrew D. Miller, Anna M. Schotthoefer, Carl Stenoien, Lucinda B. Johnson, and Val R. Beasley

    PNAS March 10, 2015 112 (10) 3008-3013; first published February 23, 2015. https://doi.org/10.1073/pnas.1415971112
  • Open Access

    Synergistic and antagonistic interactions between bednets and vaccines in the control of malaria

    • From the Cover

    Yael Artzy-Randrup, Andrew P. Dobson, and Mercedes Pascual

    PNAS March 10, 2015 112 (10) 3014-3019; first published January 20, 2015. https://doi.org/10.1073/pnas.1409467112

    See companion article:

    • Mastering malaria

      - Mar 10, 2015
  • Open Access

    Climate-driven introduction of the Black Death and successive plague reintroductions into Europe

    Boris V. Schmid, Ulf Büntgen, W. Ryan Easterday, Christian Ginzler, Lars Walløe, Barbara Bramanti, and Nils Chr. Stenseth

    PNAS March 10, 2015 112 (10) 3020-3025; first published February 23, 2015. https://doi.org/10.1073/pnas.1412887112

• Evolution

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    Evolution of herbivory in Drosophilidae linked to loss of behaviors, antennal responses, odorant receptors, and ancestral diet

    • From the Cover

    Benjamin Goldman-Huertas, Robert F. Mitchell, Richard T. Lapoint, Cécile P. Faucher, John G. Hildebrand, and Noah K. Whiteman

    PNAS March 10, 2015 112 (10) 3026-3031; first published January 26, 2015. https://doi.org/10.1073/pnas.1424656112

    See companion article:

    • Evolution of herbivory

      - Feb 25, 2015

• Genetics

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    An underlying mechanism for the increased mutagenesis of lagging-strand genes in Bacillus subtilis

    Samuel Million-Weaver, Ariana N. Samadpour, Daniela A. Moreno-Habel, Patrick Nugent, Mitchell J. Brittnacher, Eli Weiss, Hillary S. Hayden, Samuel I. Miller, Ivan Liachko, and Houra Merrikh

    PNAS March 10, 2015 112 (10) E1096-E1105; first published February 23, 2015. https://doi.org/10.1073/pnas.1416651112
  • Open Access

    Analysis of 13 cell types reveals evidence for the expression of numerous novel primate- and tissue-specific microRNAs

    Eric Londin, Phillipe Loher, Aristeidis G. Telonis, Kevin Quann, Peter Clark, Yi Jing, Eleftheria Hatzimichael, Yohei Kirino, Shozo Honda, Michelle Lally, Bharat Ramratnam, Clay E. S. Comstock, Karen E. Knudsen, Leonard Gomella, George L. Spaeth, Lisa Hark, L. Jay Katz, Agnieszka Witkiewicz, Abdolmohamad Rostami, Sergio A. Jimenez, Michael A. Hollingsworth, Jen Jen Yeh, Chad A. Shaw, Steven E. McKenzie, Paul Bray, Peter T. Nelson, Simona Zupo, Katrien Van Roosbroeck, Michael J. Keating, George A. Calin, Charles Yeo, Masaya Jimbo, Joseph Cozzitorto, Jonathan R. Brody, Kathleen Delgrosso, John S. Mattick, Paolo Fortina, and Isidore Rigoutsos

    PNAS March 10, 2015 112 (10) E1106-E1115; first published February 23, 2015. https://doi.org/10.1073/pnas.1420955112

    See companion article:

    • Identification of novel human microRNAs

      - May 15, 2015
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    Mutations in early follicular lymphoma progenitors are associated with suppressed antigen presentation

    Michael R. Green, Shingo Kihira, Chih Long Liu, Ramesh V. Nair, Raheleh Salari, Andrew J. Gentles, Jonathan Irish, Henning Stehr, Carolina Vicente-Dueñas, Isabel Romero-Camarero, Isidro Sanchez-Garcia, Sylvia K. Plevritis, Daniel A. Arber, Serafim Batzoglou, Ronald Levy, and Ash A. Alizadeh

    PNAS March 10, 2015 112 (10) E1116-E1125; first published February 23, 2015. https://doi.org/10.1073/pnas.1501199112
  • Open Access

    SWI/SNF chromatin remodeling regulates alcohol response behaviors in Caenorhabditis elegans and is associated with alcohol dependence in humans

    Laura D. Mathies, GinaMari G. Blackwell, Makeda K. Austin, Alexis C. Edwards, Brien P. Riley, Andrew G. Davies, and Jill C. Bettinger

    PNAS March 10, 2015 112 (10) 3032-3037; first published February 23, 2015. https://doi.org/10.1073/pnas.1413451112
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    Nonsense suppression by near-cognate tRNAs employs alternative base pairing at codon positions 1 and 3

    Bijoyita Roy, John D. Leszyk, David A. Mangus, and Allan Jacobson

    PNAS March 10, 2015 112 (10) 3038-3043; first published March 2, 2015. https://doi.org/10.1073/pnas.1424127112

• Immunology and Inflammation

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    Tetramers reveal IL-17–secreting CD4⁺ T cells that are specific for U1-70 in lupus and mixed connective tissue disease

    Nicole H. Kattah, Evan W. Newell, Justin Ansel Jarrell, Alvina D. Chu, Jianming Xie, Michael G. Kattah, Ofir Goldberger, Jessica Ye, Eliza F. Chakravarty, Mark M. Davis, and Paul J. Utz

    PNAS March 10, 2015 112 (10) 3044-3049; first published February 23, 2015. https://doi.org/10.1073/pnas.1424796112
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    Initial viral load determines the magnitude of the human CD8 T cell response to yellow fever vaccination

    Rama S. Akondy, Philip L. F. Johnson, Helder I. Nakaya, Srilatha Edupuganti, Mark J. Mulligan, Benton Lawson, Joseph D. Miller, Bali Pulendran, Rustom Antia, and Rafi Ahmed

    PNAS March 10, 2015 112 (10) 3050-3055; first published February 23, 2015. https://doi.org/10.1073/pnas.1500475112
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    Coincidental loss of DOCK8 function in NLRP10-deficient and C3H/HeJ mice results in defective dendritic cell migration

    Jayendra Kumar Krishnaswamy, Arpita Singh, Uthaman Gowthaman, Renee Wu, Pavane Gorrepati, Manuela Sales Nascimento, Antonia Gallman, Dong Liu, Anne Marie Rhebergen, Samuele Calabro, Lan Xu, Patricia Ranney, Anuj Srivastava, Matthew Ranson, James D. Gorham, Zachary McCaw, Steven R. Kleeberger, Leonhard X. Heinz, André C. Müller, Keiryn L. Bennett, Giulio Superti-Furga, Jorge Henao-Mejia, Fayyaz S. Sutterwala, Adam Williams, Richard A. Flavell, and Stephanie C. Eisenbarth

    PNAS March 10, 2015 112 (10) 3056-3061; first published February 23, 2015. https://doi.org/10.1073/pnas.1501554112
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    CD47 regulates the phagocytic clearance and replication of the Plasmodium yoelii malaria parasite

    Rajdeep Banerjee, Sanjay Khandelwal, Yukiko Kozakai, Bikash Sahu, and Sanjai Kumar

    PNAS March 10, 2015 112 (10) 3062-3067; first published February 23, 2015. https://doi.org/10.1073/pnas.1418144112

• Medical Sciences

  • Open Access

    Large number of rebounding/founder HIV variants emerge from multifocal infection in lymphatic tissues after treatment interruption

    Meghan K. Rothenberger, Brandon F. Keele, Stephen W. Wietgrefe, Courtney V. Fletcher, Gregory J. Beilman, Jeffrey G. Chipman, Alexander Khoruts, Jacob D. Estes, Jodi Anderson, Samuel P. Callisto, Thomas E. Schmidt, Ann Thorkelson, Cavan Reilly, Katherine Perkey, Thomas G. Reimann, Netanya S. Utay, Krystelle Nganou Makamdop, Mario Stevenson, Daniel C. Douek, Ashley T. Haase, and Timothy W. Schacker

    PNAS March 10, 2015 112 (10) E1126-E1134; first published February 23, 2015. https://doi.org/10.1073/pnas.1414926112
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    Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity

    Matteo Pasetto, Antonella Antignani, Pinar Ormanoglu, Eugen Buehler, Rajarshi Guha, Ira Pastan, Scott E. Martin, and David J. FitzGerald

    PNAS March 10, 2015 112 (10) E1135-E1142; first published February 23, 2015. https://doi.org/10.1073/pnas.1501958112
  • Open Access

    Conjugate-like immunogens produced as protein capsular matrix vaccines

    Ann Thanawastien, Robert T. Cartee, Thomas J. Griffin IV, Kevin P. Killeen, and John J. Mekalanos

    PNAS March 10, 2015 112 (10) E1143-E1151; first published February 19, 2015. https://doi.org/10.1073/pnas.1425005112
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    Detecting cancers through tumor-activatable minicircles that lead to a detectable blood biomarker

    John A. Ronald, Hui-Yen Chuang, Anca Dragulescu-Andrasi, Sharon S. Hori, and Sanjiv S. Gambhir

    PNAS March 10, 2015 112 (10) 3068-3073; first published February 23, 2015. https://doi.org/10.1073/pnas.1414156112
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    Pharmacological recruitment of aldehyde dehydrogenase 3A1 (ALDH3A1) to assist ALDH2 in acetaldehyde and ethanol metabolism in vivo

    Che-Hong Chen, Leslie A. Cruz, and Daria Mochly-Rosen

    PNAS March 10, 2015 112 (10) 3074-3079; first published February 23, 2015. https://doi.org/10.1073/pnas.1414657112
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    Transcription factor Hes1 modulates osteoarthritis development in cooperation with calcium/calmodulin-dependent protein kinase 2

    Shurei Sugita, Yoko Hosaka, Keita Okada, Daisuke Mori, Fumiko Yano, Hiroshi Kobayashi, Yuki Taniguchi, Yoshifumi Mori, Tomotake Okuma, Song Ho Chang, Manabu Kawata, Shuji Taketomi, Hirotaka Chikuda, Haruhiko Akiyama, Ryoichiro Kageyama, Ung-il Chung, Sakae Tanaka, Hiroshi Kawaguchi, Shinsuke Ohba, and Taku Saito

    PNAS March 10, 2015 112 (10) 3080-3085; first published March 2, 2015. https://doi.org/10.1073/pnas.1419699112

• Microbiology

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    Rapid selection against arbovirus-induced apoptosis during infection of a mosquito vector

    Katelyn O’Neill, Bradley J. S. C. Olson, Ning Huang, Dave Unis, and Rollie J. Clem

    PNAS March 10, 2015 112 (10) E1152-E1161; first published February 23, 2015. https://doi.org/10.1073/pnas.1424469112
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    Thiopeptide antibiotics stimulate biofilm formation in Bacillus subtilis

    Rachel Bleich, Jeramie D. Watrous, Pieter C. Dorrestein, Albert A. Bowers, and Elizabeth A. Shank

    PNAS March 10, 2015 112 (10) 3086-3091; first published February 23, 2015. https://doi.org/10.1073/pnas.1414272112
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    MinD-like ATPase FlhG effects location and number of bacterial flagella during C-ring assembly

    Jan S. Schuhmacher, Florian Rossmann, Felix Dempwolff, Carina Knauer, Florian Altegoer, Wieland Steinchen, Anja K. Dörrich, Andreas Klingl, Milena Stephan, Uwe Linne, Kai M. Thormann, and Gert Bange

    PNAS March 10, 2015 112 (10) 3092-3097; first published March 2, 2015. https://doi.org/10.1073/pnas.1419388112

• Neuroscience

  • Open Access

    Protein mutated in paroxysmal dyskinesia interacts with the active zone protein RIM and suppresses synaptic vesicle exocytosis

    • Inaugural Article

    Yiguo Shen, Woo-Ping Ge, Yulong Li, Arisa Hirano, Hsien-Yang Lee, Astrid Rohlmann, Markus Missler, Richard W. Tsien, Lily Yeh Jan, Ying-Hui Fu, and Louis J. Ptáček

    PNAS March 10, 2015 112 (10) 2935-2941; first published February 17, 2015. https://doi.org/10.1073/pnas.1501364112
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    Cross-language differences in the brain network subserving intelligible speech

    Jianqiao Ge, Gang Peng, Bingjiang Lyu, Yi Wang, Yan Zhuo, Zhendong Niu, Li Hai Tan, Alexander P. Leff, and Jia-Hong Gao

    PNAS March 10, 2015 112 (10) 2972-2977; first published February 23, 2015. https://doi.org/10.1073/pnas.1416000112
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    Interplay of approximate planning strategies

    • From the Cover

    Quentin J. M. Huys, Níall Lally, Paul Faulkner, Neir Eshel, Erich Seifritz, Samuel J. Gershman, Peter Dayan, and Jonathan P. Roiser

    PNAS March 10, 2015 112 (10) 3098-3103; first published February 9, 2015. https://doi.org/10.1073/pnas.1414219112

    See companion article:

    • Dividing and conquering the world a step at a time

      - Mar 02, 2015
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    Oxytocin prevents ethanol actions at δ subunit-containing GABA_A receptors and attenuates ethanol-induced motor impairment in rats

    Michael T. Bowen, Sebastian T. Peters, Nathan Absalom, Mary Chebib, Inga D. Neumann, and Iain S. McGregor

    PNAS March 10, 2015 112 (10) 3104-3109; first published February 23, 2015. https://doi.org/10.1073/pnas.1416900112
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    Decorrelation of retinal response to natural scenes by fixational eye movements

    Irina Yonit Segal, Chen Giladi, Michael Gedalin, Michele Rucci, Mor Ben-Tov, Yam Kushinsky, Alik Mokeichev, and Ronen Segev

    PNAS March 10, 2015 112 (10) 3110-3115; first published February 23, 2015. https://doi.org/10.1073/pnas.1412059112
  • Open Access

    Distinct populations of neurons respond to emotional valence and arousal in the human subthalamic nucleus

    Tomáš Sieger, Tereza Serranová, Filip Růžička, Pavel Vostatek, Jiří Wild, Daniela Šťastná, Cecilia Bonnet, Daniel Novák, Evžen Růžička, Dušan Urgošík, and Robert Jech

    PNAS March 10, 2015 112 (10) 3116-3121; first published February 23, 2015. https://doi.org/10.1073/pnas.1410709112
  • Open Access

    Network compensation of cyclic GMP-dependent protein kinase II knockout in the hippocampus by Ca²⁺-permeable AMPA receptors

    Seonil Kim, Roseann F. Titcombe, Hong Zhang, Latika Khatri, Hiwot K. Girma, Franz Hofmann, Ottavio Arancio, and Edward B. Ziff

    PNAS March 10, 2015 112 (10) 3122-3127; first published February 23, 2015. https://doi.org/10.1073/pnas.1417498112

• Physiology

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    Noninvasive in vivo imaging reveals differences between tectorial membrane and basilar membrane traveling waves in the mouse cochlea

    Hee Yoon Lee, Patrick D. Raphael, Jesung Park, Audrey K. Ellerbee, Brian E. Applegate, and John S. Oghalai

    PNAS March 10, 2015 112 (10) 3128-3133; first published March 3, 2015. https://doi.org/10.1073/pnas.1500038112

• Plant Biology

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    Tonoplast CBL–CIPK calcium signaling network regulates magnesium homeostasis in Arabidopsis

    • From the Cover

    Ren-Jie Tang, Fu-Geng Zhao, Veder J. Garcia, Thomas J. Kleist, Lei Yang, Hong-Xia Zhang, and Sheng Luan

    PNAS March 10, 2015 112 (10) 3134-3139; first published February 2, 2015. https://doi.org/10.1073/pnas.1420944112

    See companion article:

    • Vacuole and high magnesium stress

      - Feb 26, 2015
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    Dual role of tree florigen activation complex component FD in photoperiodic growth control and adaptive response pathways

    Szymon Tylewicz, Hiroyuki Tsuji, Pál Miskolczi, Anna Petterle, Abdul Azeez, Kristoffer Jonsson, Ko Shimamoto, and Rishikesh P. Bhalerao

    PNAS March 10, 2015 112 (10) 3140-3145; first published February 23, 2015. https://doi.org/10.1073/pnas.1423440112
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    Spatiotemporally dynamic, cell-type–dependent premeiotic and meiotic phasiRNAs in maize anthers

    Jixian Zhai, Han Zhang, Siwaret Arikit, Kun Huang, Guo-Ling Nan, Virginia Walbot, and Blake C. Meyers

    PNAS March 10, 2015 112 (10) 3146-3151; first published February 23, 2015. https://doi.org/10.1073/pnas.1418918112

• Psychological and Cognitive Sciences

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    Mother’s voice and heartbeat sounds elicit auditory plasticity in the human brain before full gestation

    • From the Cover

    Alexandra R. Webb, Howard T. Heller, Carol B. Benson, and Amir Lahav

    PNAS March 10, 2015 112 (10) 3152-3157; first published February 23, 2015. https://doi.org/10.1073/pnas.1414924112

    See companion article:

    • Auditory neuroplasticity in preterm newborns

      - Jul 13, 2015

• Systems Biology

  • Open Access

    Model-guided quantitative analysis of microRNA-mediated regulation on competing endogenous RNAs using a synthetic gene circuit

    Ye Yuan, Bing Liu, Peng Xie, Michael Q. Zhang, Yanda Li, Zhen Xie, and Xiaowo Wang

    PNAS March 10, 2015 112 (10) 3158-3163; first published February 23, 2015. https://doi.org/10.1073/pnas.1413896112
  • Open Access

    Cardiac ferroportin regulates cellular iron homeostasis and is important for cardiac function

    Samira Lakhal-Littleton, Magda Wolna, Carolyn A. Carr, Jack J. J. Miller, Helen C. Christian, Vicky Ball, Ana Santos, Rebeca Diaz, Daniel Biggs, Richard Stillion, Philip Holdship, Fiona Larner, Damian J. Tyler, Kieran Clarke, Benjamin Davies, and Peter A. Robbins

    PNAS March 10, 2015 112 (10) 3164-3169; first published February 23, 2015. https://doi.org/10.1073/pnas.1422373112