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Research Article

Silencing of end-joining repair for efficient site-specific gene insertion after TALEN/CRISPR mutagenesis in Aedes aegypti

Sanjay Basu, Azadeh Aryan, Justin M. Overcash, Glady Hazitha Samuel, Michelle A. E. Anderson, Timothy J. Dahlem, Kevin M. Myles, and Zach N. Adelman
PNAS March 31, 2015 112 (13) 4038-4043; first published March 16, 2015; https://doi.org/10.1073/pnas.1502370112
Sanjay Basu
aFralin Life Science Institute and Department of Entomology, Virginia Tech, Blacksburg, VA 24061; and
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Azadeh Aryan
aFralin Life Science Institute and Department of Entomology, Virginia Tech, Blacksburg, VA 24061; and
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Justin M. Overcash
aFralin Life Science Institute and Department of Entomology, Virginia Tech, Blacksburg, VA 24061; and
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Glady Hazitha Samuel
aFralin Life Science Institute and Department of Entomology, Virginia Tech, Blacksburg, VA 24061; and
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Michelle A. E. Anderson
aFralin Life Science Institute and Department of Entomology, Virginia Tech, Blacksburg, VA 24061; and
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Timothy J. Dahlem
bMutation Generation and Detection Core, Health Science Center Core Research Facility, University of Utah, Salt Lake City, UT 84132
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Kevin M. Myles
aFralin Life Science Institute and Department of Entomology, Virginia Tech, Blacksburg, VA 24061; and
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  • For correspondence: mylesk@vt.edu zachadel@vt.edu
Zach N. Adelman
aFralin Life Science Institute and Department of Entomology, Virginia Tech, Blacksburg, VA 24061; and
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  • For correspondence: mylesk@vt.edu zachadel@vt.edu
  1. Edited by Anthony A. James, University of California, Irvine, CA, and approved February 24, 2015 (received for review February 6, 2015)

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Article Information

vol. 112 no. 13 4038-4043
DOI: 
https://doi.org/10.1073/pnas.1502370112
PubMed: 
25775608

Published By: 
National Academy of Sciences
Print ISSN: 
0027-8424
Online ISSN: 
1091-6490
History: 
  • Published in issue March 31, 2015.
  • Published first March 16, 2015.

Article Versions

  • Previous version (March 16, 2015 - 12:03).
  • You are viewing the most recent version of this article.

Author Information

  1. Sanjay Basua,1,
  2. Azadeh Aryana,1,
  3. Justin M. Overcasha,
  4. Glady Hazitha Samuela,
  5. Michelle A. E. Andersona,
  6. Timothy J. Dahlemb,
  7. Kevin M. Mylesa,2, and
  8. Zach N. Adelmana,2
  1. aFralin Life Science Institute and Department of Entomology, Virginia Tech, Blacksburg, VA 24061; and
  2. bMutation Generation and Detection Core, Health Science Center Core Research Facility, University of Utah, Salt Lake City, UT 84132
  1. Edited by Anthony A. James, University of California, Irvine, CA, and approved February 24, 2015 (received for review February 6, 2015)

Footnotes

  • ↵1S.B. and A.A. contributed equally to this work.

  • ↵2To whom correspondence may be addressed. Email: mylesk{at}vt.edu or zachadel{at}vt.edu.
  • Author contributions: S.B., A.A., J.M.O., G.H.S., T.J.D., K.M.M., and Z.N.A. designed research; S.B., A.A., J.M.O., G.H.S., and M.A.E.A. performed research; T.J.D. contributed new reagents/analytic tools; S.B., A.A., J.M.O., G.H.S., M.A.E.A., T.J.D., K.M.M., and Z.N.A. analyzed data; and S.B., A.A., T.J.D., K.M.M., and Z.N.A. wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1502370112/-/DCSupplemental.

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    Japanese Journal of Thrombosis and Hemostasis 2015 26 5
  • Transcription Factors for Abiotic Stress Tolerance in Plants
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    2020

Article usage

Article usage: March 2015 to January 2021

AbstractFullPdf
Mar 2015304035447
Apr 20153020226332
May 20153639198
Jun 20153876367
Jul 20152205955
Aug 20153074561
Sep 20152226754
Oct 20153409094
Nov 201535796105
Dec 20152739490
Total 201585298661403
Jan 201616699102
Feb 201617172103
Mar 201618687106
Apr 20162007871
May 20161417580
Jun 20161106646
Jul 20161154862
Aug 2016854435
Sep 20161113736
Oct 20161476166
Nov 201623298111
Dec 20161375640
Total 20161801821858
Jan 20171246144
Feb 20171095132
Mar 20171105668
Apr 20171155051
May 20171045831
Jun 2017944631
Jul 2017664523
Aug 2017683638
Sep 2017875235
Oct 2017593328
Nov 2017954534
Dec 2017963021
Total 20171127563436
Jan 2018734140
Feb 2018857020
Mar 201815210642
Apr 2018927931
May 201811110027
Jun 2018746220
Jul 2018676330
Aug 201813812738
Sep 20181059138
Oct 2018267836
Nov 20182310037
Dec 2018139015
Total 20189591007374
Jan 2019105621
Feb 2019135725
Mar 2019317731
Apr 2019326234
May 2019164824
Jun 2019134114
Jul 2019183744
Aug 2019184831
Sep 2019164625
Oct 2019376929
Nov 2019235231
Dec 2019205238
Total 2019247645347
Jan 2020176625
Feb 2020124516
Mar 2020286428
Apr 2020186130
May 2020195925
Jun 2020114817
Jul 202095630
Aug 2020134035
Sep 2020145331
Oct 20203111626
Nov 2020359437
Dec 2020309248
Total 2020237794348
Jan 2021123017
Total 2021123017
Total1291247263783
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Silencing of end-joining repair for efficient site-specific gene insertion after TALEN/CRISPR mutagenesis in Aedes aegypti
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CRISPR-based gene insertion in mosquitoes
Sanjay Basu, Azadeh Aryan, Justin M. Overcash, Glady Hazitha Samuel, Michelle A. E. Anderson, Timothy J. Dahlem, Kevin M. Myles, Zach N. Adelman
Proceedings of the National Academy of Sciences Mar 2015, 112 (13) 4038-4043; DOI: 10.1073/pnas.1502370112

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CRISPR-based gene insertion in mosquitoes
Sanjay Basu, Azadeh Aryan, Justin M. Overcash, Glady Hazitha Samuel, Michelle A. E. Anderson, Timothy J. Dahlem, Kevin M. Myles, Zach N. Adelman
Proceedings of the National Academy of Sciences Mar 2015, 112 (13) 4038-4043; DOI: 10.1073/pnas.1502370112
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Proceedings of the National Academy of Sciences: 112 (13)
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