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Research Article

Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging

Akira Takai, Masahiro Nakano, Kenta Saito, Remi Haruno, Tomonobu M. Watanabe, Tatsuya Ohyanagi, Takashi Jin, Yasushi Okada, and Takeharu Nagai
PNAS April 7, 2015 112 (14) 4352-4356; first published March 23, 2015; https://doi.org/10.1073/pnas.1418468112
Akira Takai
Laboratories for aCell Polarity Regulation,
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Masahiro Nakano
bThe Institute of Scientific and Industrial Research, Osaka University, Osaka 567-0047, Japan; and
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Kenta Saito
bThe Institute of Scientific and Industrial Research, Osaka University, Osaka 567-0047, Japan; and
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Remi Haruno
bThe Institute of Scientific and Industrial Research, Osaka University, Osaka 567-0047, Japan; and
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Tomonobu M. Watanabe
cComprehensive Bioimaging,
dPRESTO, Japan Science and Technology Agency, Tokyo 102-0075, Japan
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Tatsuya Ohyanagi
eNano-Bio Probes, and
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Takashi Jin
eNano-Bio Probes, and
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Yasushi Okada
Laboratories for aCell Polarity Regulation,
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  • For correspondence: ng1@sanken.osaka-u.ac.jp y.okada@riken.jp
Takeharu Nagai
bThe Institute of Scientific and Industrial Research, Osaka University, Osaka 567-0047, Japan; and
dPRESTO, Japan Science and Technology Agency, Tokyo 102-0075, Japan
fCell Dynamics Observation, Quantitative Biology Center, RIKEN, Osaka 565-0874, Japan;
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  • For correspondence: ng1@sanken.osaka-u.ac.jp y.okada@riken.jp
  1. Edited by Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD, and approved February 18, 2015 (received for review September 25, 2014)

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Article Information

vol. 112 no. 14 4352-4356
DOI: 
https://doi.org/10.1073/pnas.1418468112
PubMed: 
25831507

Published By: 
National Academy of Sciences
Print ISSN: 
0027-8424
Online ISSN: 
1091-6490
History: 
  • Published in issue April 7, 2015.
  • Published first March 23, 2015.

Copyright & Usage: 
Freely available online through the PNAS open access option.

Author Information

  1. Akira Takaia,1,
  2. Masahiro Nakanob,1,
  3. Kenta Saitob,
  4. Remi Harunob,
  5. Tomonobu M. Watanabec,d,
  6. Tatsuya Ohyanagie,
  7. Takashi Jine,
  8. Yasushi Okadaa,2, and
  9. Takeharu Nagaib,d,f,2
  1. Laboratories for aCell Polarity Regulation,
  2. cComprehensive Bioimaging,
  3. eNano-Bio Probes, and
  4. fCell Dynamics Observation, Quantitative Biology Center, RIKEN, Osaka 565-0874, Japan;
  5. bThe Institute of Scientific and Industrial Research, Osaka University, Osaka 567-0047, Japan; and
  6. dPRESTO, Japan Science and Technology Agency, Tokyo 102-0075, Japan
  1. Edited by Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD, and approved February 18, 2015 (received for review September 25, 2014)

Footnotes

  • ↵1A.T. and M.N. contributed equally to this work.

  • ↵2To whom correspondence may be addressed. Email: ng1{at}sanken.osaka-u.ac.jp or y.okada{at}riken.jp.
  • Author contributions: A.T., M.N., K.S., Y.O., and T.N. designed research; A.T., M.N., K.S., R.H., T.M.W., Y.O., and T.N. performed research; T.O. and T.J. contributed new reagents A.T., M.N., K.S., R.H., Y.O., and T.N. analyzed data; and A.T., M.N., Y.O., and T.N. wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

  • Data deposition: The sequences reported in this paper have been deposited in the GenBank database (accession nos. AB982072–AB982104 and AB983214–AB983217).

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1418468112/-/DCSupplemental.

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Development of bright cyan and orange luciferases
Akira Takai, Masahiro Nakano, Kenta Saito, Remi Haruno, Tomonobu M. Watanabe, Tatsuya Ohyanagi, Takashi Jin, Yasushi Okada, Takeharu Nagai
Proceedings of the National Academy of Sciences Apr 2015, 112 (14) 4352-4356; DOI: 10.1073/pnas.1418468112

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Development of bright cyan and orange luciferases
Akira Takai, Masahiro Nakano, Kenta Saito, Remi Haruno, Tomonobu M. Watanabe, Tatsuya Ohyanagi, Takashi Jin, Yasushi Okada, Takeharu Nagai
Proceedings of the National Academy of Sciences Apr 2015, 112 (14) 4352-4356; DOI: 10.1073/pnas.1418468112
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