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Research Article

Activity-dependent, homeostatic regulation of neurotransmitter release from auditory nerve fibers

Tenzin Ngodup, Jack A. Goetz, Brian C. McGuire, Wei Sun, Amanda M. Lauer, and Matthew A. Xu-Friedman
  1. aDepartment of Biological Sciences, University at Buffalo, State University of New York, Buffalo, NY 14260;
  2. bCenter for Hearing and Balance and Department of Otolaryngology-Head and Neck Surgery, The Johns Hopkins University, Baltimore, MD 21205; and
  3. cCenter for Hearing and Deafness, Department of Communicative Disorders and Sciences, University at Buffalo, State University of New York, Buffalo, NY 14214

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PNAS May 19, 2015 112 (20) 6479-6484; first published May 5, 2015; https://doi.org/10.1073/pnas.1420885112
Tenzin Ngodup
aDepartment of Biological Sciences, University at Buffalo, State University of New York, Buffalo, NY 14260;
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Jack A. Goetz
aDepartment of Biological Sciences, University at Buffalo, State University of New York, Buffalo, NY 14260;
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Brian C. McGuire
bCenter for Hearing and Balance and Department of Otolaryngology-Head and Neck Surgery, The Johns Hopkins University, Baltimore, MD 21205; and
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Wei Sun
cCenter for Hearing and Deafness, Department of Communicative Disorders and Sciences, University at Buffalo, State University of New York, Buffalo, NY 14214
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Amanda M. Lauer
bCenter for Hearing and Balance and Department of Otolaryngology-Head and Neck Surgery, The Johns Hopkins University, Baltimore, MD 21205; and
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Matthew A. Xu-Friedman
aDepartment of Biological Sciences, University at Buffalo, State University of New York, Buffalo, NY 14260;
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  • For correspondence: mx@buffalo.edu
  1. Edited by Gina G. Turrigiano, Brandeis University, Waltham, MA, and approved April 16, 2015 (received for review November 3, 2014)

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Significance

Synapses with high probability of neurotransmitter release (Pr) depress during prolonged activity, which reduces the faithful transfer of information. Auditory nerve synapses onto bushy cells show particularly strong depression at physiologically relevant rates of activity, which raises the question of how bushy cells transmit information when sound levels are high for a prolonged period. After rearing mice in constant, nondamaging noise, auditory nerve synapses changed from high to low Pr, with a corresponding increase in the number of release sites, which increased spike fidelity during high activity. Neither quantal size nor average excitatory postsynaptic current changed. After returning to control conditions, Pr recovered to high. These changes seem to reflect a homeostatic response to enhance fidelity.

Abstract

Information processing in the brain requires reliable synaptic transmission. High reliability at specialized auditory nerve synapses in the cochlear nucleus results from many release sites (N), high probability of neurotransmitter release (Pr), and large quantal size (Q). However, high Pr also causes auditory nerve synapses to depress strongly when activated at normal rates for a prolonged period, which reduces fidelity. We studied how synapses are influenced by prolonged activity by exposing mice to constant, nondamaging noise and found that auditory nerve synapses changed to facilitating, reflecting low Pr. For mice returned to quiet, synapses recovered to normal depression, suggesting that these changes are a homeostatic response to activity. Two additional properties, Q and average excitatory postsynaptic current (EPSC) amplitude, were unaffected by noise rearing, suggesting that the number of release sites (N) must increase to compensate for decreased Pr. These changes in N and Pr were confirmed physiologically using the integration method. Furthermore, consistent with increased N, endbulbs in noise-reared animals had larger VGlut1-positive puncta, larger profiles in electron micrographs, and more release sites per profile. In current-clamp recordings, noise-reared BCs had greater spike fidelity even during high rates of synaptic activity. Thus, auditory nerve synapses regulate excitability through an activity-dependent, homeostatic mechanism, which could have major effects on all downstream processing. Our results also suggest that noise-exposed bushy cells would remain hyperexcitable for a period after returning to normal quiet conditions, which could have perceptual consequences.

  • homeostasis
  • release probability
  • cochlear nucleus

Synapses must be able to transmit information reliably over a range of activity levels. A critical factor in regulating this fidelity is the probability of neurotransmitter release (Pr). If Pr is too high, synapses may have high fidelity at low rates of activity, but they would strongly depress at high rates, which could reduce postsynaptic spiking. If Pr is too low, failure to release neurotransmitter could reduce fidelity.

Activity can influence synaptic properties through multiple mechanisms. One mechanism, homeostatic synaptic scaling, is an activity-dependent regulation of quantal size (Q) (1⇓–3), which has recently been reported to occur in vivo (4, 5). One limitation is that changes in Q do not change the degree of synaptic depression. Furthermore, increases in Q could be limited by receptor number or density. In dissociated hippocampal cell cultures, activity-dependent changes in Pr have been observed (6⇓–8). However, it is not known whether such changes in Pr occur in vivo in the intact brain. In addition, it is not clear how modulation of Pr could prevent depression without also causing large changes in the magnitude of the excitatory postsynaptic current (EPSC).

Fidelity is critical for auditory nerve (AN) synapses onto bushy cells (BCs) in the anteroventral cochlear nucleus (AVCN). AN fibers fire at rates up to 100 Hz spontaneously, and as high as 300 Hz when driven by sound (9⇓⇓–12). At these rates, AN synapses (called endbulbs of Held) show significant depression, which can lead to loss of spiking in BCs (13⇓⇓⇓⇓–18). It is therefore a question how endbulbs transmit information when activity levels are high for extended periods, such as in noisy environments, or in AN fibers with high sensitivity, and whether there are activity-dependent processes that offset depression. Changes in the AVCN and its avian homolog nucleus magnocellularis are observed after such manipulations as extremely intense noise and cochlear or genetic ablation (19⇓⇓⇓⇓⇓–25). However, these manipulations or their effects seem irreversible so it is difficult to assess whether the changes are homeostatic responses to low activity levels.

Here, we show that sound-driven activity regulates Pr in vivo in the auditory pathway at endbulbs. Endbulbs from animals reared in constant noise exhibited less depression and even facilitation. Pr recovered back to high after returning animals to normal sound conditions. We observed no change in Q, but rather a compensatory increase in the number of release sites, N. Furthermore, BCs from noise-reared animals had higher firing probability during high rates of fiber stimulation. Thus, activity seems to drive homeostatic changes in Pr and N at the first stage of the auditory pathway.

Results

Changes in Pr.

To assess Pr, we recorded from BCs in whole-cell voltage clamp, isolated individual presynaptic AN fibers, and delivered pairs of stimuli with various intervals. A representative recording is shown in Fig. 1A, Left, with the endbulb showing typically strong depression at intervals up to 20 ms, reflecting high Pr. From these data, we calculated the paired-pulse ratio (PPR) by normalizing the amplitude of the second EPSC to the first (PPR = EPSC2/EPSC1) (Fig. 1B). PPR was below 1 at all interpulse intervals (Δt) (Fig. 1B, open circles). Similar results were found in 28 cells in this study (Fig. 1C) and are consistent with previous recordings from BCs confirmed by cell filling (14).

Fig. 1.
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Fig. 1.

Noise exposure lowers Pr at the endbulb of Held. AN fibers were stimulated with pairs of pulses of different interpulse intervals, Δt = 3–20 ms. (A) Representative traces recorded from P26 BCs from control and noise-reared animals. In control conditions (Left), EPSCs show depression at all intervals whereas, after noise rearing (Right), EPSCs show no depression, but rather facilitation. (B) Paired-pulse ratio (PPR) for the representative experiments in A. (C) Average PPR for control (n = 17) and noise-reared (n = 22) in animals P21 or older. PPR differed significantly (P < 0.005) at all intervals. (D) PPR for control (n = 28) and noise-reared (n = 26) at Δt = 3 ms as a function of age. Noise rearing commenced at P12. (E) PPR at Δt = 3 ms for control (open circles) and recovery (closed triangles) BCs. (F) Average PPR is slightly elevated over control after recovery (control n = 15; recovery n = 20, P < 0.05 for each interval). (G) Cumulative frequency of EPSC1 amplitudes in BCs from control (n = 34) and noise-reared animals (n = 22). The amplitudes do not differ significantly (P > 0.5, Kolmogorov–Smirnov test). Averages in C and F and in subsequent figures are represented by the mean ± the SEM.

We tested the impact of activity on Pr by exposing mice to constant noise beginning at postnatal day 12 (P12) and assessing Pr beginning at P18. The example endbulb in Fig. 1 A, Right and B showed no depression, but rather facilitation for interpulse intervals from 3 to 20 ms. This PPR is well above the range observed under normal conditions. Recordings from a number of BCs showed significant increases in PPR after noise rearing. We confirmed the cell identity using neurobiotin in the intracellular solution in some experiments (control n = 12, noise-reared n = 13) (Fig. S1), and cell structure seemed normal (26⇓⇓⇓⇓⇓–32). PPR for Δt = 3 ms was 0.61 ± 0.04 in control (n = 17) whereas, after noise rearing, PPR was 0.93 ± 0.06 (n = 22), which is significantly higher (P = 0.005, Student’s unpaired t test). PPRs at all intervals up to 20 ms were significantly higher (P < 0.05) (Fig. 1 B and C). The increase in PPR was first observed around P22, during which time PPR is relatively stable in control synapses (Fig. 1D). An increase in PPR results from a decrease in Pr so these results suggest that activity plays an important role in setting Pr.

We next tested whether synapses were permanently altered by exposing mice to noise until P26, then allowing recovery in normal sound conditions for at least 2 wk. After restoration of a normal sound environment, the PPR eventually recovered to near normal levels (Fig. 1E) although it remained significantly higher than control at all intervals. For Δt = 3 ms, PPR after recovery was 0.767 ± 0.031 (n = 20), which was significantly higher than control BCs (PPR = 0.606 ± 0.028, n = 15, P < 0.05, t test) (Fig. 1F). Thus, Pr decreased in noise and increased back near normal levels when normal sound conditions were restored, suggesting that changes in Pr are adaptive and depend on sensory experience.

We tested the effects of noise rearing on general auditory function using auditory brainstem responses (ABRs). There were no significant changes in either threshold or amplitudes of the individual ABR components (Fig. S2). We also assessed hair cell synapses onto auditory nerve afferents by counting CtBP2-immunopositive synaptic ribbons. There was no significant change after noise rearing in the number of ribbons per inner hair cell in base, middle, or apical regions of the basilar membrane (Fig. S3). There were also no noticeable changes in the positions of the synaptic ribbons. Thus, the levels of noise we used for these experiments did not cause any visible, permanent hearing loss or damage to the cochlea.

We also assessed how noise rearing affected the first EPSC (EPSC1) in each pair. There were no significant changes in half-width (control, 0.43 ± 0.01 ms, n = 34; noise-reared, 0.42 ± 0.01 ms, n = 22, P > 0.5) or in decay τ (control, 0.186 ± 0.006 ms, n = 34; noise-reared, 0.174 ± 0.007 ms, n = 22, P > 0.2). These values fit well within the range that we have observed before (14). In addition, the distribution of EPSC1 amplitudes was not significantly changed by noise rearing [P > 0.5, Kolmogorov–Smirnov (K–S) test] (Fig. 1G). This lack of change in EPSC1 is surprising because, all else being equal, a decrease in Pr should cause a decrease in EPSC1 amplitude. Therefore, it appears that other factors compensate for the decrease in Pr.

We first considered the quantal size (Q) because it can change homeostatically with activity (1). We recorded miniature EPSCs (mEPSCs) from BCs of control and noise-reared mice. The representative cells in Fig. 2A had similar mEPSC frequency (control = 6.4 events per s; noise-reared = 5.3 events per s), similar average mEPSC amplitude (control, 80 pA; noise-reared, 96 pA), and similar distributions of mEPSC amplitude (Fig. 2B). On average, noise-reared BCs were not significantly different from control in average mEPSC amplitude (control, 89 ± 5 pA, n = 19; noise-reared, 96 ± 6 pA, n = 12, P = 0.30, K–S test) (Fig. 2C) or frequency (control, 3.5 ± 0.32/s, n = 19; noise-reared, 4.8 ± 0.75/s, n = 12, P = 0.37, K–S test) (Fig. 2D). Thus, AN synapses did not show obvious homeostatic changes in Q with activity, indicating that another factor must compensate for the decrease in Pr, most likely the number of release sites, N.

Fig. 2.
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Fig. 2.

Noise rearing has no effect on mEPSCs. (A) Representative traces showing mEPSCs measured in BCs from control (Top) and noise-reared (Bottom) animals. (B) Cumulative histogram of mEPSC amplitude for the representative cells in A. (C) Cumulative histogram of average mEPSC amplitudes measured in BCs from control (n = 19) and noise-reared (n = 12) animals. The distributions do not differ significantly (P = 0.3, K–S test). (D) Cumulative histogram of mEPSC frequency in BCs from control (n = 19) and noise-reared (n = 12) animals. The distributions of frequency do not differ significantly (P = 0.37). (E) Representative EPSC trains recorded in the presence of 1 mM kynurenate from a control and noise-reared BC in response to stimulation of a single presynaptic AN fiber (45 pulses, 100 Hz). (F) Derivation of N and Pr from trains data in E, using the integration method. EPSC amplitudes are integrated, and a fit is extrapolated back to the y axis. Arrowheads mark the estimated value of N. Pr is calculated by dividing the first EPSC by N. (G) Average Pr and N measured using the integration method. Noise-reared endbulbs have significantly lower Pr (0.30 ± 0.01, P < 0.005) and larger N (10.1 ± 0.1 nA, P < 0.05) compared with control (Pr = 0.45 ± 0.04, N = 5.9 ± 1.2 nA).

To verify quantitative changes in Pr and N, we used the integration method (20, 33⇓–35). Single AN inputs were stimulated with a long train (45 pulses, 100 Hz) (Fig. 2E) in the presence of 1 mM kynurenate to prevent AMPA receptor saturation and desensitization. The peak amplitudes of the evoked EPSCs were integrated (markers in Fig. 2F), and the steady-state rate of replenishment was estimated by a straight-line fit to the last 20 pulses of the cumulative EPSC (lines in Fig. 2F). The y intercept of the fit is proportional to N (arrows in Fig. 2F), which significantly increased after noise rearing (12 control endbulbs, 5.9 ± 1.2 nA vs. 9 noise-reared, 10.1 ± 1.0 nA, P < 0.05) (Fig. 2G, black bars). Pr is calculated by dividing the first EPSC in the train by N. Pr decreased significantly after noise rearing (control, 0.45 ± 0.04 vs. noise-reared, 0.30 ± 0.01, P < 0.005) (Fig. 2G, white bars). These data reinforce the conclusions based on PPR and EPSC1 data that noise rearing leads to a decrease in Pr and an increase in N.

Anatomical Changes.

We considered whether there were changes in synaptic structure consistent with an increase in N. AN synapses are primarily axosomatic (36⇓–38). To quantify such changes, we immunolabeled endbulbs using an antibody against vesicular glutamate transporter-1 (VGLUT-1), which shows strong punctate staining around the BC soma (Fig. 3 A and B) (30, 38⇓–40). These puncta represent areas of the endbulb containing releasable vesicles, and thus the areas containing release sites. We quantified the average cross-sectional area of puncta in the optical section taken at the midline of each cell. The sizes of puncta around BCs from noise-reared animals were larger than those from controls (control, 1.71 ± 0.18 µm2, n = 10; noise-reared, 2.37 ± 0.25 µm2, n = 15; P < 0.05, t test) (Fig. 3C). This increase in synaptic area is consistent with an increase in the number of release sites.

Fig. 3.
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Fig. 3.

Anatomical changes at light and ultrastructure levels. (A and B) Endbulbs immunolabeled for VGLUT-1 in control (A) and noise-reared (B) animals. BC somata are surrounded by multiple VGLUT-1–positive puncta. (Scale bar: A and B, 20 µm.) (C) Puncta area for endbulbs from control and noise-reared mice. Horizontal lines mark average areas of puncta around individual BCs, and bars are overall averages across all cells (10 control, 15 noise-reared). The average area of puncta around noise-reared BCs is significantly greater than around control BCs (P < 0.05). (D and E) Representative profiles of endbulbs from control (D) and noise-reared (E) animals. Each profile has numerous synaptic vesicles, curved and asymmetric postsynaptic densities (asterisks). (Scale bar: D and E, 1 µm.) (F–H) Endbulbs in noise-reared mice had significantly more postsynaptic densities per profile (P < 0.05, Fisher exact test; F), larger profile perimeter and cross-sectional area (P < 0.001; G) and larger form factors (P < 0.001; H).

To gain a better understanding of the ultrastructural changes underlying the larger area in VGLUT-1 puncta, we examined electron micrographs. AN synapses from control animals resembled those described previously for the CBA/CaJ strain (30). Each section through an endbulb (a “profile”) displayed clusters of mitochondria, numerous large round synaptic vesicles, and curved, asymmetric postsynaptic densities (Fig. 3 D and E). Extended extracellular spaces, mitochondrion adherens complexes, and puncta adherentia were sometimes observed in profiles. Glial sheaths surrounded the synaptic terminals. AN profiles from noise-reared animals seemed generally similar to control.

We quantified differences between quiet- and noise-reared mice (n = 22 profiles from control mice, and n = 33 profiles from noise-reared mice). Noise-reared mice had more postsynaptic densities (PSDs) per profile (P < 0.05, Fisher exact test) (Fig. 3F), larger profile cross-sectional areas (control, 1.23 ± 0.14 µm2 vs. noise-reared, 2.59 ± 0.32 µm2, P < 0.001, t test) (Fig. 3G), and larger form factors (area/perimeter, control, 0.19 ± 0.01 µm vs. noise-reared, 0.27 ± 0.02 µm, t test, P < 0.001) (Fig. 3H). Thus, noise-reared endbulbs show clear changes in structure that are consistent with an increase in release site density.

Postsynaptic Effects.

Sound-driven activity can influence postsynaptic excitability in other parts of the auditory pathway, such as the medial nucleus of the trapezoid body (41). We examined how noise rearing might influence the intrinsic properties of BCs in the AVCN. Representative responses to depolarizing current injection for control and noise-reared BCs are shown in Fig. 4. Spikes maintained the undershooting amplitude characteristic of BCs (Fig. 4A). There were small, but significant, shifts in peak of AP (control, −18.10 ± 2.06 mV, n = 30, vs. noise-reared, −22.61 ± 1.38 mV, n = 32, P = 0.03, t test) and threshold voltage (control, −42.49 ± 0.79 mV vs. noise-reared, −45.3 ± 0.77 mV, P < 0.005, t test) (Fig. 4B). Spikes in BCs from noise-reared animals had shorter half-width than control BCs (control, 0.76 ± 0.08 ms, n = 15 vs. noise-reared, 0.54 ± 0.05 ms, n = 26, P = 0.03). We saw no significant difference in the resting membrane potential (control, −56 ± 1 mV; noise-reared, −57 ± 0.5 mV, P > 0.05) between control and noise-reared BCs. We also saw no significant change in the input resistance (Fig. S4). The decrease in spike threshold, together with the speeding of the action potential and the decreased synaptic depression, might enhance the likelihood of BCs to respond more reliably during high rates of synaptic activity.

Fig. 4.
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Fig. 4.

Effects of noise rearing on spike generation and fidelity of BC spiking. (A) Representative current-clamp recordings in response to current pulses of 0.6 nA in control and noise-reared BCs. (B) Average measurements of 30 BCs from control and 32 BCs from noise-reared mice, showing significant decreases in action potential peak voltage (Top, P = 0.03), threshold voltage (Middle, P < 0.005), and spike half-width (Bottom, P < 0.05). (C) Representative traces showing responses to fiber stimulation at 100 (Top) and 333 Hz (Bottom) in BCs from control (Left) and noise-reared (Right) mice. (D) Cumulative frequency histogram of spike probability (Pspike) over the second half of the stimulation train (pulses 11–20), for experiments similar to C. Pspike increased significantly after noise rearing (P < 0.05, asterisks) for both 100 (i) and 333 (ii) Hz.

We tested these possibilities directly by doing fiber stimulation in current-clamp recordings. We activated AN fibers using trains of stimuli at different frequencies (100, 200, and 333 Hz). In control conditions, BCs fired reliably early in the trains of stimuli but failed to fire at later pulses, especially for the 333 Hz stimulation rate (Fig. 4C, Left), presumably because of synaptic depression. However, in noise-reared BCs, spiking was much more reliable for those later pulses (Fig. 4C, Right). When we quantified spike probability for pulses 11‒20, it was much higher for noise-reared BCs at all frequencies tested (K–S test, P < 0.05) (Fig. 4D). We also quantified spike latency and jitter, but neither was affected significantly by noise rearing (P > 0.1) (Fig. S4). Thus, spike reliability increased greatly after noise rearing.

Discussion

We have found that sound-driven activity regulates the endbulb of Held by causing a decrease in Pr and an increase in N. These changes in Pr and N seem to be homeostatic, adaptive responses to noise exposure. The decrease in Pr reduces vesicle depletion during high rates of activity, and N increases to compensate, so that the initial EPSC remains of similar amplitude. We saw no changes in quantal size that would have been consistent with synaptic scaling. Recovery experiments suggest that endbulbs adapt their Pr and N to different sound conditions for at least several weeks after the onset of hearing. These changes seem functionally relevant because noise exposure increased the spiking probability in BCs even during high levels of activity. There could be consequences for auditory processing because the endbulb of Held is a critical gateway for auditory activity entering the brain.

Activity can influence a number of synaptic factors homeostatically. Changes in quantal size, Q, were identified in cultured cells (42, 43) and more recently in vivo (4, 5). In the auditory system, deafferentation or mutations that compromise hearing have been linked to changes in synaptic transmission and postsynaptic excitability (19, 21, 22, 25). In mutants of the synaptic protein Bassoon, auditory nerve activity decreased, endbulb Q and Pr both increased, and N decreased (25). In dn/dn mutants, which are deaf because of mutations in TMC1 (44), Pr increased, but neither Q nor N changed (21). These results seem largely to complement our observation that increased auditory activity led to decreased Pr and increased N although we observed no evidence of changes in Q, indicating that synaptic scaling may not be a universal mechanism. It is unclear why these changes differ in their details, but one possibility is that genetic manipulation had additional consequences. Excitability can also change through channel expression, phosphorylation, or localization (22, 41, 45). Similar changes likely also take place in BCs in response to noise exposure and could underlie the changes we saw in action potential duration and threshold. Activity in cultured hippocampal cells can also influence Pr (6) although the timescale of this phenomenon seems much faster than that in endbulbs, which retain the decrease in Pr over the course of many hours associated with slice preparation and recording. Furthermore, our findings indicated that the change in Pr in endbulbs was accompanied by a compensatory change in N, and these changes can be recruited in vivo.

It is striking that the average initial EPSC did not change in amplitude even though Pr decreased considerably, indicating not only that N increased to compensate, but also that N increased by just the right amount. Similarly, the average mEPSC rate did not change. This rate is a function of both the number of release sites (i.e., N) and the tendency of each to release, which has been linked to Pr. Thus, these two aspects of synaptic transmission seem to be linked, despite their independent physiological basis. Further experiments will be required to determine whether they are regulated in tandem, or whether changes in one trigger changes in the other through a separate mechanism.

Our experiments reinforce the idea that Pr is not an inconsequential characteristic of synapses. On the contrary, it is tightly regulated so that different synapses show different levels of Pr, even for synapses formed by the same axon (46). Our results further indicate that Pr is regulated depending on the activity level. Even so, Pr can show considerable variability within a single population of synapses, including among auditory nerve fibers (47). The mechanism we have found may help to account for this natural variability: AN fibers with higher activity levels would have lower Pr than those with lower activity. Activity levels in different AN fibers are also highly variable, which correlates with sensitivity to sound (9, 48). We predict that these different classes of AN fiber also have different Pr in the AVCN. Indeed, AN fibers with similar activity levels (i.e., similar intensity sensitivity) seem to converge on the same BC (49). We showed previously that endbulbs that converge on the same BC have similar Pr (50), suggesting that ANs sort onto BCs in a very precise way, presumably to analyze different characteristics of sounds.

The functional consequences of the changes that we observed are dramatic. BCs from noise-reared animals had much higher spiking probability even during high rates of activation. It is interesting to consider possible changes in perception. Decreased Pr could permit sound-driven activity to survive depression at the endbulb, improving perception of sounds even in loud environments. However, when animals return to quiet conditions, activity levels that normally trigger depression and spike failure in quiet-reared animals would cause spikes after noise rearing. Similar increases in excitability have been associated with tinnitus (51⇓–53), and hyperactivity has been seen in inferior colliculus (54), but the cellular origin of this hyperactivity is not clear. Similarly, auditory experience can influence response properties in primary auditory cortex (55⇓⇓–58). Our results indicate that changes as early as the cochlear nucleus also happen and could contribute to the changes seen at higher levels.

It is unlikely that cochlear damage from noise exposure could account for our results. Cochlear damage may not affect ABR threshold, yet can be detected as changes in individual ABR peak amplitudes (59). We saw no changes in ABR threshold, individual ABR peaks, number of ribbons per hair cell, or synaptic ultrastructure after noise treatment. Furthermore, we saw recovery to normal levels of Pr after removing animals from noise. Thus, changes in Pr seem to be specific, adaptive changes in synaptic function in response to elevated levels of activity. Interestingly, we also found that noise exposure increased the spiking probability in BCs even during high levels of activity, which could have strong functional consequences for auditory perception when animals return to quiet conditions.

Materials and Methods

All procedures were approved by the University at Buffalo’s Institutional Animal Care and Use Committee. Experiments used CBA/CaJ mice (The Jackson Laboratory) of either sex. Noise exposure was started at P12 (1.6–39 kHz frequency range, 90–94 dB) using a white noise generator (ACO Pacific 3025). Slices of AVCN were prepared at P18–P62, using methods described previously (60). Electrophysiological recordings were made at 34 °C. BCs were identified in voltage clamp by EPSCs with rapid decay kinetics (τ < 0.2 ms) and half-width ≤ 0.5 ms (14), and in current clamp by their undershooting spikes (61). Individual AN fibers were stimulated using a microelectrode placed 30‒50 µm away from the soma (A365, WPI). For VGLUT-1 immunohistochemistry, slices containing the AVCN were incubated with primary anti–VGLUT-1 antibodies (1:500; Invitrogen), followed by Texas-red–conjugated goat anti-rabbit secondary (1:200; Jackson ImmunoResearch Laboratories). For electron microscopy, thin sections were cut at 75–100 nm. AN synapses were recognized by characteristic large, round synaptic vesicles, clear cytoplasm, and identifiable postsynaptic densities. Ultrastructural features were measured using ImageJ. See SI Materials and Methods for more details.

Acknowledgments

We thank H. Graham for assistance analyzing transmission electron microscopy data, A. Siegel and W. Sigurdson for assistance with confocal images, T. Moser, S. Jung, and D. Ding for advice about CtBP2 immunolabeling, and R. La Rosa, Y. Yang, H. Yang, and X. Zhuang for helpful comments on the manuscript. These experiments were supported by National Institutes of Health (NIH) Grants R03 012352 (to A.M.L.) and R01 DC008125 (to M.A.X.-F.), National Science Foundation Grant 1208131 (to M.A.X.-F.), and the Dalai Lama Trust Fund (T.N.). These experiments were also supported by NIH P30 Grants DC005211 (to the Center for Hearing and Balance) and EY001765 (to the Wilmer Eye Institute Imaging at Johns Hopkins University).

Footnotes

  • ↵1To whom correspondence should be addressed. Email: mx{at}buffalo.edu.
  • Author contributions: T.N., W.S., A.M.L., and M.A.X.-F. designed research; T.N., J.A.G., B.C.M., W.S., and A.M.L. performed research; T.N., J.A.G., B.C.M., W.S., A.M.L., and M.A.X.-F. analyzed data; and T.N., A.M.L., and M.A.X.-F. wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1420885112/-/DCSupplemental.

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Homeostatic regulation of release probability
Tenzin Ngodup, Jack A. Goetz, Brian C. McGuire, Wei Sun, Amanda M. Lauer, Matthew A. Xu-Friedman
Proceedings of the National Academy of Sciences May 2015, 112 (20) 6479-6484; DOI: 10.1073/pnas.1420885112

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Homeostatic regulation of release probability
Tenzin Ngodup, Jack A. Goetz, Brian C. McGuire, Wei Sun, Amanda M. Lauer, Matthew A. Xu-Friedman
Proceedings of the National Academy of Sciences May 2015, 112 (20) 6479-6484; DOI: 10.1073/pnas.1420885112
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