Functional network inference of the suprachiasmatic nucleus

John H. Abel; Kirsten Meeker; Daniel Granados-Fuentes; Peter C. St. John; Thomas J. Wang; Benjamin B. Bales; Francis J. Doyle; Erik D. Herzog; Linda R. Petzold

doi:10.1073/pnas.1521178113

New Research In

Edited by Joseph S. Takahashi, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, and approved March 7, 2016 (received for review November 3, 2015)

This article has Corrections. Please see:

Correction for Abel et al., Functional network inference of the suprachiasmatic nucleus - October 23, 2017
Correction to Supporting Information for Abel et al., Functional network inference of the suprachiasmatic nucleus - October 23, 2017

Significance

In mammals, circadian rhythms are controlled by a network of neurons in the brain. The structure of this network dictates organism-wide behavior and adaptation to the environment. We used a neurotoxin to desynchronize this circadian network and then used tools from information theory to determine which cells communicate to establish synchronization. Our results show that this functional network consists of two densely-connected cores, surrounded by sparsely connected shell regions. These findings represent the first time, to our knowledge, that this network has been examined at single cell resolution and show that the importance of these core network regions is independent of light input.

Abstract

In the mammalian suprachiasmatic nucleus (SCN), noisy cellular oscillators communicate within a neuronal network to generate precise system-wide circadian rhythms. Although the intracellular genetic oscillator and intercellular biochemical coupling mechanisms have been examined previously, the network topology driving synchronization of the SCN has not been elucidated. This network has been particularly challenging to probe, due to its oscillatory components and slow coupling timescale. In this work, we investigated the SCN network at a single-cell resolution through a chemically induced desynchronization. We then inferred functional connections in the SCN by applying the maximal information coefficient statistic to bioluminescence reporter data from individual neurons while they resynchronized their circadian cycling. Our results demonstrate that the functional network of circadian cells associated with resynchronization has small-world characteristics, with a node degree distribution that is exponential. We show that hubs of this small-world network are preferentially located in the central SCN, with sparsely connected shells surrounding these cores. Finally, we used two computational models of circadian neurons to validate our predictions of network structure.

Footnotes

↵¹J.H.A. and K.M. contributed equally to this work.
↵²To whom correspondence should be addressed. Email: petzold{at}engineering.ucsb.edu.

Author contributions: J.H.A., K.M., D.G.-F., P.C.S.J., T.J.W., F.J.D., E.D.H., and L.R.P. designed research; J.H.A., K.M., D.G.-F., P.C.S.J., T.J.W., B.B.B., and E.D.H. performed research; J.H.A., K.M., D.G.-F., P.C.S.J., B.B.B., F.J.D., E.D.H., and L.R.P. analyzed data; and J.H.A., B.B.B., and E.D.H. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1521178113/-/DCSupplemental.

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