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Research Article

Mice carrying a human GLUD2 gene recapitulate aspects of human transcriptome and metabolome development

Qian Li, Song Guo, Xi Jiang, Jaroslaw Bryk, Ronald Naumann, Wolfgang Enard, Masaru Tomita, Masahiro Sugimoto, Philipp Khaitovich, and Svante Pääbo
  1. aChinese Academy of Sciences Key Laboratory of Computational Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031 Shanghai, China;
  2. bUniversity of Chinese Academy of Sciences, 100049 Beijing, China;
  3. cMax Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany;
  4. dMax Planck Institute of Molecular Cell Biology and Genetics, D-01307 Dresden, Germany;
  5. eInstitute for Advanced Biosciences, Keio University, 997-0035 Tsuruoka, Yamagata, Japan;
  6. fSkolkovo Institute for Science and Technology, 143025 Skolkovo, Russia

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PNAS May 10, 2016 113 (19) 5358-5363; first published April 26, 2016; https://doi.org/10.1073/pnas.1519261113
Qian Li
aChinese Academy of Sciences Key Laboratory of Computational Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031 Shanghai, China;
bUniversity of Chinese Academy of Sciences, 100049 Beijing, China;
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Song Guo
aChinese Academy of Sciences Key Laboratory of Computational Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031 Shanghai, China;
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Xi Jiang
aChinese Academy of Sciences Key Laboratory of Computational Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031 Shanghai, China;
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Jaroslaw Bryk
cMax Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany;
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Ronald Naumann
dMax Planck Institute of Molecular Cell Biology and Genetics, D-01307 Dresden, Germany;
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Wolfgang Enard
cMax Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany;
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Masaru Tomita
eInstitute for Advanced Biosciences, Keio University, 997-0035 Tsuruoka, Yamagata, Japan;
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Masahiro Sugimoto
eInstitute for Advanced Biosciences, Keio University, 997-0035 Tsuruoka, Yamagata, Japan;
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Philipp Khaitovich
aChinese Academy of Sciences Key Laboratory of Computational Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031 Shanghai, China;
cMax Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany;
fSkolkovo Institute for Science and Technology, 143025 Skolkovo, Russia
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  • For correspondence: khaitovich@eva.mpg.de paabo@eva.mpg.de
Svante Pääbo
cMax Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany;
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  • For correspondence: khaitovich@eva.mpg.de paabo@eva.mpg.de
  1. Edited by Joshua M. Akey, University of Washington, Seattle, WA, and accepted by the Editorial Board April 1, 2016 (received for review September 28, 2015)

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Significance

A novel version of the glutamate dehydrogenase gene, GLUD2, evolved in the common ancestors of humans and apes. Based on sequence and expression pattern, GLUD2 has been suggested to play a role in glutamate metabolism in human and ape brains. We have generated transgenic mice carrying a human GLUD2 gene. Analysis of transcriptome and metabolome changes induced by GLUD2 in the cerebral cortex revealed no changes in glutamate concentration but instead changes to metabolic pathways centering on the TCA cycle during early postnatal development. These changes mirrored differences seen between human and macaque during cortex development, suggesting that GLUD2 may play a role during brain development in apes and humans, possibly by providing precursors for the biosynthesis of lipids.

Abstract

Whereas all mammals have one glutamate dehydrogenase gene (GLUD1), humans and apes carry an additional gene (GLUD2), which encodes an enzyme with distinct biochemical properties. We inserted a bacterial artificial chromosome containing the human GLUD2 gene into mice and analyzed the resulting changes in the transcriptome and metabolome during postnatal brain development. Effects were most pronounced early postnatally, and predominantly genes involved in neuronal development were affected. Remarkably, the effects in the transgenic mice partially parallel the transcriptome and metabolome differences seen between humans and macaques analyzed. Notably, the introduction of GLUD2 did not affect glutamate levels in mice, consistent with observations in the primates. Instead, the metabolic effects of GLUD2 center on the tricarboxylic acid cycle, suggesting that GLUD2 affects carbon flux during early brain development, possibly supporting lipid biosynthesis.

  • human evolution
  • GLUD2
  • brain metabolism

Footnotes

  • ↵1Q.L. and S.G. contributed equally to this work.

  • ↵2Present address: School of Applied Sciences, University of Huddersfield, HD1 3DH Huddersfield, UK.

  • ↵3Present address: Department of Biology II, Ludwig Maximilians University, 82152 Martinsried, Germany.

  • ↵4To whom correspondence may be addressed. Email: khaitovich{at}eva.mpg.de or paabo{at}eva.mpg.de.
  • Author contributions: W.E., P.K., and S.P. designed research; X.J., J.B., R.N., and M.S. performed research; W.E., M.T., and M.S. contributed reagents/analytic tools; Q.L. and S.G. analyzed data; and Q.L., P.K., and S.P. wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission. J.M.A. is a guest editor invited by the Editorial Board.

  • Data deposition: The data reported in this paper have been deposited in the Gene Expression Omnibus (GEO) database, www.ncbi.nlm.nih.gov/geo (accession no. GSE80122).

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1519261113/-/DCSupplemental.

Freely available online through the PNAS open access option.

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Survey of GLUD2 function using transgenic mice
Qian Li, Song Guo, Xi Jiang, Jaroslaw Bryk, Ronald Naumann, Wolfgang Enard, Masaru Tomita, Masahiro Sugimoto, Philipp Khaitovich, Svante Pääbo
Proceedings of the National Academy of Sciences May 2016, 113 (19) 5358-5363; DOI: 10.1073/pnas.1519261113

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Survey of GLUD2 function using transgenic mice
Qian Li, Song Guo, Xi Jiang, Jaroslaw Bryk, Ronald Naumann, Wolfgang Enard, Masaru Tomita, Masahiro Sugimoto, Philipp Khaitovich, Svante Pääbo
Proceedings of the National Academy of Sciences May 2016, 113 (19) 5358-5363; DOI: 10.1073/pnas.1519261113
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