Table of Contents

Biological Sciences

• Applied Biological Sciences

  • Open Access

    Genetically encoded sensors enable real-time observation of metabolite production

    Jameson K. Rogers and George M. Church

    PNAS March 1, 2016 113 (9) 2388-2393; first published February 8, 2016. https://doi.org/10.1073/pnas.1600375113

• Biochemistry

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    Bioactive cell-like hybrids coassembled from (glyco)dendrimersomes with bacterial membranes

    Qi Xiao, Srujana S. Yadavalli, Shaodong Zhang, Samuel E. Sherman, Elodie Fiorin, Louise da Silva, Daniela A. Wilson, Daniel A. Hammer, Sabine André, Hans-Joachim Gabius, Michael L. Klein, Mark Goulian, and Virgil Percec

    PNAS March 1, 2016 113 (9) E1134-E1141; first published February 16, 2016. https://doi.org/10.1073/pnas.1525589113
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    Characterization and small-molecule stabilization of the multisite tandem binding between 14-3-3 and the R domain of CFTR

    Loes M. Stevers, Chan V. Lam, Seppe F. R. Leysen, Femke A. Meijer, Daphne S. van Scheppingen, Rens M. J. M. de Vries, Graeme W. Carlile, Lech G. Milroy, David Y. Thomas, Luc Brunsveld, and Christian Ottmann

    PNAS March 1, 2016 113 (9) E1152-E1161; first published February 17, 2016. https://doi.org/10.1073/pnas.1516631113
  • Open Access

    Calcium can mobilize and activate myosin-VI

    • From the Cover

    Christopher Batters, Dario Brack, Heike Ellrich, Beate Averbeck, and Claudia Veigel

    PNAS March 1, 2016 113 (9) E1162-E1169; first published January 25, 2016. https://doi.org/10.1073/pnas.1519435113

    See companion article:

    • Calcium gets myosin VI ready for work

      - Feb 19, 2016
  • Open Access

    Single-molecule imaging reveals the mechanism of Exo1 regulation by single-stranded DNA binding proteins

    Logan R. Myler, Ignacio F. Gallardo, Yi Zhou, Fade Gong, Soo-Hyun Yang, Marc S. Wold, Kyle M. Miller, Tanya T. Paull, and Ilya J. Finkelstein

    PNAS March 1, 2016 113 (9) E1170-E1179; first published February 16, 2016. https://doi.org/10.1073/pnas.1516674113
  • Open Access

    70S-scanning initiation is a novel and frequent initiation mode of ribosomal translation in bacteria

    Hiroshi Yamamoto, Daniela Wittek, Romi Gupta, Bo Qin, Takuya Ueda, Roland Krause, Kaori Yamamoto, Renate Albrecht, Markus Pech, and Knud H. Nierhaus

    PNAS March 1, 2016 113 (9) E1180-E1189; first published February 17, 2016. https://doi.org/10.1073/pnas.1524554113
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    Mechanism of a cytosolic O-glycosyltransferase essential for the synthesis of a bacterial adhesion protein

    Yu Chen, Ravin Seepersaud, Barbara A. Bensing, Paul M. Sullam, and Tom A. Rapoport

    PNAS March 1, 2016 113 (9) E1190-E1199; first published February 16, 2016. https://doi.org/10.1073/pnas.1600494113
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    First structure of full-length mammalian phenylalanine hydroxylase reveals the architecture of an autoinhibited tetramer

    Emilia C. Arturo, Kushol Gupta, Annie Héroux, Linda Stith, Penelope J. Cross, Emily J. Parker, Patrick J. Loll, and Eileen K. Jaffe

    PNAS March 1, 2016 113 (9) 2394-2399; first published February 16, 2016. https://doi.org/10.1073/pnas.1516967113
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    A protein constructed de novo enables cell growth by altering gene regulation

    Katherine M. Digianantonio and Michael H. Hecht

    PNAS March 1, 2016 113 (9) 2400-2405; first published February 16, 2016. https://doi.org/10.1073/pnas.1600566113

• Biophysics and Computational Biology

  • Open Access

    Novel actin filaments from Bacillus thuringiensis form nanotubules for plasmid DNA segregation

    Shimin Jiang, Akihiro Narita, David Popp, Umesh Ghoshdastider, Lin Jie Lee, Ramanujam Srinivasan, Mohan K. Balasubramanian, Toshiro Oda, Fujiet Koh, Mårten Larsson, and Robert C. Robinson

    PNAS March 1, 2016 113 (9) E1200-E1205; first published February 12, 2016. https://doi.org/10.1073/pnas.1600129113
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    Kinetic model of the aggregation of alpha-synuclein provides insights into prion-like spreading

    Marija Iljina, Gonzalo A. Garcia, Mathew H. Horrocks, Laura Tosatto, Minee L. Choi, Kristina A. Ganzinger, Andrey Y. Abramov, Sonia Gandhi, Nicholas W. Wood, Nunilo Cremades, Christopher M. Dobson, Tuomas P. J. Knowles, and David Klenerman

    PNAS March 1, 2016 113 (9) E1206-E1215; first published February 16, 2016. https://doi.org/10.1073/pnas.1524128113
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    Conserved properties of individual Ca²⁺-binding sites in calmodulin

    D. Brent Halling, Benjamin J. Liebeskind, Amelia W. Hall, and Richard W. Aldrich

    PNAS March 1, 2016 113 (9) E1216-E1225; first published February 16, 2016. https://doi.org/10.1073/pnas.1600385113
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    Enzyme catalysis by entropy without Circe effect

    • From the Cover

    Masoud Kazemi, Fahmi Himo, and Johan Åqvist

    PNAS March 1, 2016 113 (9) 2406-2411; first published January 11, 2016. https://doi.org/10.1073/pnas.1521020113

    See companion article:

    • Dispelling Circe's effect in enzyme catalysis

      - Feb 16, 2016
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    Load-dependent destabilization of the γ-rotor shaft in F_OF₁ ATP synthase revealed by hydrogen/deuterium-exchange mass spectrometry

    Siavash Vahidi, Yumin Bi, Stanley D. Dunn, and Lars Konermann

    PNAS March 1, 2016 113 (9) 2412-2417; first published February 16, 2016. https://doi.org/10.1073/pnas.1520464113

• Cell Biology

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    MEKK2 mediates an alternative β-catenin pathway that promotes bone formation

    Matthew Blake Greenblatt, Dong Yeon Shin, Hwanhee Oh, Ki-Young Lee, Bo Zhai, Steven P. Gygi, Sutada Lotinun, Roland Baron, Dou Liu, Bing Su, Laurie H. Glimcher, and Jae-Hyuck Shim

    PNAS March 1, 2016 113 (9) E1226-E1235; first published February 16, 2016. https://doi.org/10.1073/pnas.1600813113
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    The light chains of kinesin-1 are autoinhibited

    Yan Y. Yip, Stefano Pernigo, Anneri Sanger, Mengjia Xu, Maddy Parsons, Roberto A. Steiner, and Mark P. Dodding

    PNAS March 1, 2016 113 (9) 2418-2423; first published February 16, 2016. https://doi.org/10.1073/pnas.1520817113
  • Open Access

    Dynamic secretion during meiotic reentry integrates the function of the oocyte and cumulus cells

    Hakan Cakmak, Federica Franciosi, A. Musa Zamah, Marcelle I. Cedars, and Marco Conti

    PNAS March 1, 2016 113 (9) 2424-2429; first published February 10, 2016. https://doi.org/10.1073/pnas.1519990113

• Environmental Sciences

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    Oyster reproduction is affected by exposure to polystyrene microplastics

    • From the Cover

    Rossana Sussarellu, Marc Suquet, Yoann Thomas, Christophe Lambert, Caroline Fabioux, Marie Eve Julie Pernet, Nelly Le Goïc, Virgile Quillien, Christian Mingant, Yanouk Epelboin, Charlotte Corporeau, Julien Guyomarch, Johan Robbens, Ika Paul-Pont, Philippe Soudant, and Arnaud Huvet

    PNAS March 1, 2016 113 (9) 2430-2435; first published February 1, 2016. https://doi.org/10.1073/pnas.1519019113

    See companion article:

    • Marine microplastics affect future generations

      - Feb 22, 2016

    See companion article:

    • Realistic scenario for microplastic impact studies

      - Jul 12, 2016
  • Open Access

    Illuminating structural proteins in viral “dark matter” with metaproteomics

    Jennifer R. Brum, J. Cesar Ignacio-Espinoza, Eun-Hae Kim, Gareth Trubl, Robert M. Jones, Simon Roux, Nathan C. VerBerkmoes, Virginia I. Rich, and Matthew B. Sullivan

    PNAS March 1, 2016 113 (9) 2436-2441; first published February 16, 2016. https://doi.org/10.1073/pnas.1525139113

• Evolution

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    Delayed fungal evolution did not cause the Paleozoic peak in coal production

    • From the Cover

    Matthew P. Nelsen, William A. DiMichele, Shanan E. Peters, and C. Kevin Boyce

    PNAS March 1, 2016 113 (9) 2442-2447; first published January 19, 2016. https://doi.org/10.1073/pnas.1517943113

    See companion article:

    • A Late Paleozoic climate window of opportunity

      - Feb 16, 2016
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    Competition between anthocyanin and flavonol biosynthesis produces spatial pattern variation of floral pigments between Mimulus species

    Yao-Wu Yuan, Alexandra B. Rebocho, Janelle M. Sagawa, Lauren E. Stanley, and Harvey D. Bradshaw Jr.

    PNAS March 1, 2016 113 (9) 2448-2453; first published February 16, 2016. https://doi.org/10.1073/pnas.1515294113

• Genetics

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    Deep phenotyping of 89 xeroderma pigmentosum patients reveals unexpected heterogeneity dependent on the precise molecular defect

    Hiva Fassihi, Mieran Sethi, Heather Fawcett, Jonathan Wing, Natalie Chandler, Shehla Mohammed, Emma Craythorne, Ana M. S. Morley, Rongxuan Lim, Sally Turner, Tanya Henshaw, Isabel Garrood, Paola Giunti, Tammy Hedderly, Adesoji Abiona, Harsha Naik, Gemma Harrop, David McGibbon, Nicolaas G. J. Jaspers, Elena Botta, Tiziana Nardo, Miria Stefanini, Antony R. Young, Robert P. E. Sarkany, and Alan R. Lehmann

    PNAS March 1, 2016 113 (9) E1236-E1245; first published February 16, 2016. https://doi.org/10.1073/pnas.1519444113
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    Phagocytosis genes nonautonomously promote developmental cell death in the Drosophila ovary

    Allison K. Timmons, Albert A. Mondragon, Claire E. Schenkel, Alla Yalonetskaya, Jeffrey D. Taylor, Katherine E. Moynihan, Jon Iker Etchegaray, Tracy L. Meehan, and Kimberly McCall

    PNAS March 1, 2016 113 (9) E1246-E1255; first published February 16, 2016. https://doi.org/10.1073/pnas.1522830113
  • Open Access

    Sex-specific regulation of Lgr3 in Drosophila neurons

    Geoffrey W. Meissner, Shengzhan D. Luo, Brian G. Dias, Michael J. Texada, and Bruce S. Baker

    PNAS March 1, 2016 113 (9) E1256-E1265; first published February 16, 2016. https://doi.org/10.1073/pnas.1600241113
  • Open Access

    Visualizing the origins of selfish de novo mutations in individual seminiferous tubules of human testes

    Geoffrey J. Maher, Simon J. McGowan, Eleni Giannoulatou, Clare Verrill, Anne Goriely, and Andrew O. M. Wilkie

    PNAS March 1, 2016 113 (9) 2454-2459; first published February 8, 2016. https://doi.org/10.1073/pnas.1521325113

• Immunology and Inflammation

  • Open Access

    Cholesteryl esters stabilize human CD1c conformations for recognition by self-reactive T cells

    Salah Mansour, Anna S. Tocheva, Chris Cave-Ayland, Moritz M. Machelett, Barbara Sander, Nikolai M. Lissin, Peter E. Molloy, Mark S. Baird, Gunthard Stübs, Nicolas W. J. Schröder, Ralf R. Schumann, Jörg Rademann, Anthony D. Postle, Bent K. Jakobsen, Ben G. Marshall, Rajendra Gosain, Paul T. Elkington, Tim Elliott, Chris-Kriton Skylaris, Jonathan W. Essex, Ivo Tews, and Stephan D. Gadola

    PNAS March 1, 2016 113 (9) E1266-E1275; first published February 16, 2016. https://doi.org/10.1073/pnas.1519246113
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    How structural adaptability exists alongside HLA-A2 bias in the human αβ TCR repertoire

    Sydney J. Blevins, Brian G. Pierce, Nishant K. Singh, Timothy P. Riley, Yuan Wang, Timothy T. Spear, Michael I. Nishimura, Zhiping Weng, and Brian M. Baker

    PNAS March 1, 2016 113 (9) E1276-E1285; first published February 16, 2016. https://doi.org/10.1073/pnas.1522069113
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    Successful immunotherapy induces previously unidentified allergen-specific CD4+ T-cell subsets

    John F. Ryan, Rachel Hovde, Jacob Glanville, Shu-Chen Lyu, Xuhuai Ji, Sheena Gupta, Robert J. Tibshirani, David C. Jay, Scott D. Boyd, R. Sharon Chinthrajah, Mark M. Davis, Stephen J. Galli, Holden T. Maecker, and Kari C. Nadeau

    PNAS March 1, 2016 113 (9) E1286-E1295; first published January 25, 2016. https://doi.org/10.1073/pnas.1520180113
  • Open Access

    CD8⁺CD122⁺CD49d^low regulatory T cells maintain T-cell homeostasis by killing activated T cells via Fas/FasL-mediated cytotoxicity

    Kazuyuki Akane, Seiji Kojima, Tak W. Mak, Hiroshi Shiku, and Haruhiko Suzuki

    PNAS March 1, 2016 113 (9) 2460-2465; first published February 11, 2016. https://doi.org/10.1073/pnas.1525098113

• Medical Sciences

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    Truncating PREX2 mutations activate its GEF activity and alter gene expression regulation in NRAS-mutant melanoma

    Yonathan Lissanu Deribe, Yanxia Shi, Kunal Rai, Luigi Nezi, Samir B. Amin, Chia-Chin Wu, Kadir C. Akdemir, Mozhdeh Mahdavi, Qian Peng, Qing Edward Chang, Kirsti Hornigold, Stefan T. Arold, Heidi C. E. Welch, Levi A. Garraway, and Lynda Chin

    PNAS March 1, 2016 113 (9) E1296-E1305; first published February 16, 2016. https://doi.org/10.1073/pnas.1513801113
  • Open Access

    AAVP displaying octreotide for ligand-directed therapeutic transgene delivery in neuroendocrine tumors of the pancreas

    Tracey L. Smith, Ziqiang Yuan, Marina Cardó-Vila, Carmen Sanchez Claros, Asha Adem, Min-Hui Cui, Craig A. Branch, Juri G. Gelovani, Steven K. Libutti, Richard L. Sidman, Renata Pasqualini, and Wadih Arap

    PNAS March 1, 2016 113 (9) 2466-2471; first published February 16, 2016. https://doi.org/10.1073/pnas.1525709113
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    Gene control of tyrosine kinase TIE2 and vascular manifestations of infections

    Chandra C. Ghosh, Sascha David, Ruyang Zhang, Anthony Berghelli, Katelyn Milam, Sarah J. Higgins, Jon Hunter, Aditi Mukherjee, Yongyue Wei, Mei Tran, Freeman Suber, Lester Kobzik, Kevin C. Kain, Shulin Lu, Ansgar Santel, Kiichiro Yano, Prajna Guha, Daniel J. Dumont, David C. Christiani, and Samir M. Parikh

    PNAS March 1, 2016 113 (9) 2472-2477; first published February 16, 2016. https://doi.org/10.1073/pnas.1519467113

• Microbiology

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    Probiotics modulated gut microbiota suppresses hepatocellular carcinoma growth in mice

    Jun Li, Cecilia Ying Ju Sung, Nikki Lee, Yueqiong Ni, Jussi Pihlajamäki, Gianni Panagiotou, and Hani El-Nezami

    PNAS March 1, 2016 113 (9) E1306-E1315; first published February 16, 2016. https://doi.org/10.1073/pnas.1518189113
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    Differential regulation of type III secretion and virulence genes in Bordetella pertussis and Bordetella bronchiseptica by a secreted anti-σ factor

    • Inaugural Article

    Umesh Ahuja, Bhumika Shokeen, Ning Cheng, Yeonjoo Cho, Charles Blum, Giovanni Coppola, and Jeff F. Miller

    PNAS March 1, 2016 113 (9) 2341-2348; first published February 16, 2016. https://doi.org/10.1073/pnas.1600320113
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    A virus of hyperthermophilic archaea with a unique architecture among DNA viruses

    Elena Ilka Rensen, Tomohiro Mochizuki, Emmanuelle Quemin, Stefan Schouten, Mart Krupovic, and David Prangishvili

    PNAS March 1, 2016 113 (9) 2478-2483; first published February 16, 2016. https://doi.org/10.1073/pnas.1518929113
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    Proteomics of HCV virions reveals an essential role for the nucleoporin Nup98 in virus morphogenesis

    Marion Lussignol, Martina Kopp, Kelly Molloy, Gema Vizcay-Barrena, Roland A. Fleck, Marcus Dorner, Kierstin L. Bell, Brian T. Chait, Charles M. Rice, and Maria Teresa Catanese

    PNAS March 1, 2016 113 (9) 2484-2489; first published February 16, 2016. https://doi.org/10.1073/pnas.1518934113
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    CnaA domains in bacterial pili are efficient dissipaters of large mechanical shocks

    Daniel J. Echelman, Jorge Alegre-Cebollada, Carmen L. Badilla, Chungyu Chang, Hung Ton-That, and Julio M. Fernández

    PNAS March 1, 2016 113 (9) 2490-2495; first published February 16, 2016. https://doi.org/10.1073/pnas.1522946113
  • Open Access

    The archaeal Ced system imports DNA

    Marleen van Wolferen, Alexander Wagner, Chris van der Does, and Sonja-Verena Albers

    PNAS March 1, 2016 113 (9) 2496-2501; first published February 16, 2016. https://doi.org/10.1073/pnas.1513740113
  • Open Access

    A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species

    Ákos Nyerges, Bálint Csörgő, István Nagy, Balázs Bálint, Péter Bihari, Viktória Lázár, Gábor Apjok, Kinga Umenhoffer, Balázs Bogos, György Pósfai, and Csaba Pál

    PNAS March 1, 2016 113 (9) 2502-2507; first published February 16, 2016. https://doi.org/10.1073/pnas.1520040113

• Neuroscience

  • Open Access

    The adaptive immune system restrains Alzheimer’s disease pathogenesis by modulating microglial function

    Samuel E. Marsh, Edsel M. Abud, Anita Lakatos, Alborz Karimzadeh, Stephen T. Yeung, Hayk Davtyan, Gianna M. Fote, Lydia Lau, Jason G. Weinger, Thomas E. Lane, Matthew A. Inlay, Wayne W. Poon, and Mathew Blurton-Jones

    PNAS March 1, 2016 113 (9) E1316-E1325; first published February 16, 2016. https://doi.org/10.1073/pnas.1525466113
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    Midbrain auditory selectivity to natural sounds

    Melville J. Wohlgemuth and Cynthia F. Moss

    PNAS March 1, 2016 113 (9) 2508-2513; first published February 16, 2016. https://doi.org/10.1073/pnas.1517451113
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    Targeted axonal import (TAxI) peptide delivers functional proteins into spinal cord motor neurons after peripheral administration

    • From the Cover

    Drew L. Sellers, Jamie M. Bergen, Russell N. Johnson, Heidi Back, John M. Ravits, Philip J. Horner, and Suzie H. Pun

    PNAS March 1, 2016 113 (9) 2514-2519; first published February 17, 2016. https://doi.org/10.1073/pnas.1515526113
  • Open Access

    Consumption of palatable food primes food approach behavior by rapidly increasing synaptic density in the VTA

    Shuai Liu, Andrea K. Globa, Fergil Mills, Lindsay Naef, Min Qiao, Shernaz X. Bamji, and Stephanie L. Borgland

    PNAS March 1, 2016 113 (9) 2520-2525; first published February 16, 2016. https://doi.org/10.1073/pnas.1515724113

• Plant Biology

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    Probing the promiscuity of ent-kaurene oxidases via combinatorial biosynthesis

    Sibongile Mafu, Meirong Jia, Jiachen Zi, Dana Morrone, Yisheng Wu, Meimei Xu, Matthew L. Hillwig, and Reuben J. Peters

    PNAS March 1, 2016 113 (9) 2526-2531; first published February 16, 2016. https://doi.org/10.1073/pnas.1512096113

• Psychological and Cognitive Sciences

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    Brain size predicts problem-solving ability in mammalian carnivores

    Sarah Benson-Amram, Ben Dantzer, Gregory Stricker, Eli M. Swanson, and Kay E. Holekamp

    PNAS March 1, 2016 113 (9) 2532-2537; first published January 25, 2016. https://doi.org/10.1073/pnas.1505913113
  • Open Access

    Slow dissolving of emotional distress contributes to hyperarousal

    Rick Wassing, Jeroen S. Benjamins, Kim Dekker, Sarah Moens, Kai Spiegelhalder, Bernd Feige, Dieter Riemann, Sophie van der Sluis, Ysbrand D. Van Der Werf, Lucia M. Talamini, Matthew P. Walker, Frans Schalkwijk, and Eus J. W. Van Someren

    PNAS March 1, 2016 113 (9) 2538-2543; first published February 8, 2016. https://doi.org/10.1073/pnas.1522520113

• Systems Biology

  • Open Access

    Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM

    Alan S. L. Wong, Gigi C. G. Choi, Cheryl H. Cui, Gabriela Pregernig, Pamela Milani, Miriam Adam, Samuel D. Perli, Samuel W. Kazer, Aleth Gaillard, Mario Hermann, Alex K. Shalek, Ernest Fraenkel, and Timothy K. Lu

    PNAS March 1, 2016 113 (9) 2544-2549; first published February 10, 2016. https://doi.org/10.1073/pnas.1517883113