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Research Article

Cryo-EM structure of the replisome reveals multiple interactions coordinating DNA synthesis

Arkadiusz W. Kulczyk, Arne Moeller, Peter Meyer, View ORCID ProfilePiotr Sliz, and Charles C. Richardson
PNAS March 7, 2017 114 (10) E1848-E1856; first published February 21, 2017; https://doi.org/10.1073/pnas.1701252114
Arkadiusz W. Kulczyk
aDepartment of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115;
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  • For correspondence: arek@hms.harvard.edu ccr@hms.harvard.edu
Arne Moeller
bDepartment of Molecular Biology and Genetics, The Danish Research Institute of Translational Neuroscience, Aarhus University, 8000 Aarhus, Denmark
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Peter Meyer
aDepartment of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115;
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Piotr Sliz
aDepartment of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115;
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  • ORCID record for Piotr Sliz
Charles C. Richardson
aDepartment of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115;
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  • For correspondence: arek@hms.harvard.edu ccr@hms.harvard.edu
  1. Contributed by Charles C. Richardson, January 27, 2017 (sent for review December 7, 2016; reviewed by James M. Berger and Nicholas E. Dixon)

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Article Information

vol. 114 no. 10 E1848-E1856
DOI: 
https://doi.org/10.1073/pnas.1701252114
PubMed: 
28223502

Published By: 
National Academy of Sciences
Print ISSN: 
0027-8424
Online ISSN: 
1091-6490
History: 
  • Published in issue March 7, 2017.
  • Published first February 21, 2017.

Article Versions

  • Previous version (February 21, 2017 - 10:14).
  • You are viewing the most recent version of this article.

Author Information

  1. Arkadiusz W. Kulczyka,1,
  2. Arne Moellerb,2,
  3. Peter Meyera,
  4. Piotr Sliza, and
  5. Charles C. Richardsona,1
  1. aDepartment of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115;
  2. bDepartment of Molecular Biology and Genetics, The Danish Research Institute of Translational Neuroscience, Aarhus University, 8000 Aarhus, Denmark
  1. Contributed by Charles C. Richardson, January 27, 2017 (sent for review December 7, 2016; reviewed by James M. Berger and Nicholas E. Dixon)

Footnotes

  • ↵1To whom correspondence may be addressed. Email: arek{at}hms.harvard.edu or ccr{at}hms.harvard.edu.
  • ↵2Present address: Department of Structural Biology, Max Planck Institute of Biophysics, 60538 Frankfurt, Germany.

  • Author contributions: A.W.K. and C.C.R. designed research; A.W.K. performed research; A.W.K., A.M., P.M., and P.S. contributed new reagents/analytic tools; A.W.K. and C.C.R. analyzed data; and A.W.K. and C.C.R. wrote the paper.

  • Reviewers: J.M.B., Johns Hopkins University School of Medicine; and N.E.D., University of Wollongong.

  • The authors declare no conflict of interest.

  • Data deposition: The cryo-EM map of the replisome was deposited in the EM Data Bank, www.emdatabank.org (accession no. EMD-8565).

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1701252114/-/DCSupplemental.

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Article usage

Article usage: February 2017 to January 2021

AbstractFullPdf
Feb 20171810157210
Mar 20173289199318
Apr 20174568797
May 20171974545
Jun 20174431218
Jul 2017185368
Aug 2017931615
Sep 2017901627
Oct 2017924652
Nov 2017613431
Dec 2017433232
Total 20176759680853
Jan 2018623624
Feb 2018516326
Mar 2018778752
Apr 2018588449
May 2018455321
Jun 2018707529
Jul 2018425524
Aug 2018354917
Sep 2018404635
Oct 201898642
Nov 201869331
Dec 201847714
Total 2018499804364
Jan 201958826
Feb 201958133
Mar 201969428
Apr 2019611156
May 201916318
Jun 201917214
Jul 201905324
Aug 201933925
Sep 201916234
Oct 201968929
Nov 201926812
Dec 201967420
Total 201942894319
Jan 2020106022
Feb 202068460
Mar 20201411556
Apr 2020497148
May 202014120193
Jun 20202102121
Jul 202088284
Aug 202057390
Sep 20201159112
Oct 2020824197
Nov 2020925492
Dec 2020621858
Total 20208716051133
Jan 2021514833
Total 2021514833
Total739241312702
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Cryo-EM structure of the replisome
Arkadiusz W. Kulczyk, Arne Moeller, Peter Meyer, Piotr Sliz, Charles C. Richardson
Proceedings of the National Academy of Sciences Mar 2017, 114 (10) E1848-E1856; DOI: 10.1073/pnas.1701252114

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Cryo-EM structure of the replisome
Arkadiusz W. Kulczyk, Arne Moeller, Peter Meyer, Piotr Sliz, Charles C. Richardson
Proceedings of the National Academy of Sciences Mar 2017, 114 (10) E1848-E1856; DOI: 10.1073/pnas.1701252114
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