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Research Article

Optical visualization and quantification of enzyme activity using dynamic droplet lenses

Lauren D. Zarzar, Julia A. Kalow, Xinping He, Joseph J. Walish, and Timothy M. Swager
PNAS April 11, 2017 114 (15) 3821-3825; first published March 27, 2017; https://doi.org/10.1073/pnas.1618807114
Lauren D. Zarzar
aDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139;
bInstitute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA 02139
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Julia A. Kalow
aDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139;
bInstitute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA 02139
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Xinping He
aDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139;
bInstitute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA 02139
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Joseph J. Walish
aDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139;
bInstitute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA 02139
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Timothy M. Swager
aDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139;
bInstitute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA 02139
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  • For correspondence: tswager@mit.edu
  1. Edited by David A. Weitz, Harvard University, Cambridge, MA, and approved March 7, 2017 (received for review November 14, 2016)

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Article Information

vol. 114 no. 15 3821-3825
DOI: 
https://doi.org/10.1073/pnas.1618807114
PubMed: 
28348236

Published By: 
National Academy of Sciences
Print ISSN: 
0027-8424
Online ISSN: 
1091-6490
History: 
  • Published in issue April 11, 2017.
  • Published first March 27, 2017.

Copyright & Usage: 

Author Information

  1. Lauren D. Zarzara,b,1,
  2. Julia A. Kalowa,b,2,
  3. Xinping Hea,b,3,
  4. Joseph J. Walisha,b, and
  5. Timothy M. Swagera,b,4
  1. aDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139;
  2. bInstitute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA 02139
  1. Edited by David A. Weitz, Harvard University, Cambridge, MA, and approved March 7, 2017 (received for review November 14, 2016)

Footnotes

  • ↵1Present address: Department of Materials Science and Engineering and Department of Chemistry, The Pennsylvania State University, University Park, PA 16802.

  • ↵2Present address: Department of Chemistry, Northwestern University, Evanston, IL 60208.

  • ↵3Present address: Department of Chemical Engineering, Tianjin University, Tianjin 300072, People's Republic of China.

  • ↵4To whom correspondence should be addressed. Email: tswager{at}mit.edu.
  • Author contributions: L.D.Z., J.A.K., and T.M.S. designed research; L.D.Z., J.A.K., X.H., and J.J.W. performed research; L.D.Z., J.A.K., and T.M.S. analyzed data; and L.D.Z., J.A.K., and T.M.S. wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1618807114/-/DCSupplemental.

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Article usage

Article usage: March 2017 to January 2021

AbstractFullPdf
Mar 201713124482
Apr 2017397584260
May 20174281853
Jun 20172361516
Jul 20174281034
Aug 20173102845
Sep 20171561023
Oct 20171651327
Nov 20171232221
Dec 20171412337
Total 20177274267598
Jan 20181374537
Feb 2018524039
Mar 2018897155
Apr 2018736351
May 20181117742
Jun 2018785121
Jul 2018966436
Aug 20181126842
Sep 2018967428
Oct 2018426744
Nov 2018287634
Dec 2018207034
Total 2018934766463
Jan 2019224032
Feb 2019273629
Mar 2019315450
Apr 2019257579
May 2019183873
Jun 2019124448
Jul 2019136651
Aug 2019175341
Sep 2019227536
Oct 2019149359
Nov 2019136238
Dec 201994818
Total 2019223684554
Jan 2020144124
Feb 202083133
Mar 2020127840
Apr 2020236973
May 202097333
Jun 202085330
Jul 2020115842
Aug 2020276734
Sep 2020196148
Oct 2020156239
Nov 20202410037
Dec 2020284649
Total 2020198739482
Jan 202132113
Total 202132113
Total863224772110
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Enzyme activity using dynamic droplet lenses
Lauren D. Zarzar, Julia A. Kalow, Xinping He, Joseph J. Walish, Timothy M. Swager
Proceedings of the National Academy of Sciences Apr 2017, 114 (15) 3821-3825; DOI: 10.1073/pnas.1618807114

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Enzyme activity using dynamic droplet lenses
Lauren D. Zarzar, Julia A. Kalow, Xinping He, Joseph J. Walish, Timothy M. Swager
Proceedings of the National Academy of Sciences Apr 2017, 114 (15) 3821-3825; DOI: 10.1073/pnas.1618807114
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