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Quantitative assessment of passive electrical properties of the cardiac T-tubular system by FRAP microscopy

M. Scardigli, C. Crocini, C. Ferrantini, T. Gabbrielli, L. Silvestri, R. Coppini, C. Tesi, E. A. Rog-Zielinska, P. Kohl, E. Cerbai, C. Poggesi, F. S. Pavone, and L. Sacconi
PNAS May 30, 2017 114 (22) 5737-5742; published ahead of print May 15, 2017 https://doi.org/10.1073/pnas.1702188114
M. Scardigli
aEuropean Laboratory for Non-Linear Spectroscopy, 50019 Sesto Fiorentino, Italy;bNational Institute of Optics, National Research Council, 50019 Sesto Fiorentino, Italy;
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C. Crocini
aEuropean Laboratory for Non-Linear Spectroscopy, 50019 Sesto Fiorentino, Italy;bNational Institute of Optics, National Research Council, 50019 Sesto Fiorentino, Italy;
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  • ORCID record for C. Crocini
C. Ferrantini
cDivision of Physiology, Department of Experimental and Clinical Medicine, University of Florence, 50121 Florence, Italy;
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T. Gabbrielli
aEuropean Laboratory for Non-Linear Spectroscopy, 50019 Sesto Fiorentino, Italy;
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L. Silvestri
aEuropean Laboratory for Non-Linear Spectroscopy, 50019 Sesto Fiorentino, Italy;bNational Institute of Optics, National Research Council, 50019 Sesto Fiorentino, Italy;
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R. Coppini
dDivision of Pharmacology, Dipartimento di Neuroscienze, Psicologia, Area del Farmaco e Salute del Bambino, University of Florence, 50139 Florence, Italy;
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C. Tesi
cDivision of Physiology, Department of Experimental and Clinical Medicine, University of Florence, 50121 Florence, Italy;
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E. A. Rog-Zielinska
eInstitute for Experimental Cardiovascular Medicine, University Heart Center Freiburg–Bad Krozingen, Faculty of Medicine, University of Freiburg, 79110 Freiburg, Germany;fThe Magdi Yacoub Institute, National Heart and Lung Institute, Imperial College London, UB9 6JH London, United Kingdom;
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P. Kohl
eInstitute for Experimental Cardiovascular Medicine, University Heart Center Freiburg–Bad Krozingen, Faculty of Medicine, University of Freiburg, 79110 Freiburg, Germany;fThe Magdi Yacoub Institute, National Heart and Lung Institute, Imperial College London, UB9 6JH London, United Kingdom;
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E. Cerbai
dDivision of Pharmacology, Dipartimento di Neuroscienze, Psicologia, Area del Farmaco e Salute del Bambino, University of Florence, 50139 Florence, Italy;
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C. Poggesi
cDivision of Physiology, Department of Experimental and Clinical Medicine, University of Florence, 50121 Florence, Italy;
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F. S. Pavone
aEuropean Laboratory for Non-Linear Spectroscopy, 50019 Sesto Fiorentino, Italy;bNational Institute of Optics, National Research Council, 50019 Sesto Fiorentino, Italy;gDepartment of Physics and Astronomy, University of Florence, 50019 Florence, Italy
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L. Sacconi
aEuropean Laboratory for Non-Linear Spectroscopy, 50019 Sesto Fiorentino, Italy;bNational Institute of Optics, National Research Council, 50019 Sesto Fiorentino, Italy;
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  • For correspondence: sacconi@lens.unifi.it
  1. Edited by Clara Franzini-Armstrong, University of Pennsylvania Medical Center, Philadelphia, PA, and approved April 14, 2017 (received for review February 9, 2017)

See related content:

  • Reply to Entcheva: The impact of T-tubules on action potential propagation in cardiac tissue
    - Jan 23, 2018

This article has a reply. Please see:

  • Uncovering an electrically heterogeneous cardiomyocyte by FRAP-quantified diffusion in the T-tubules
  • Article
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Article Information

vol. 114 no. 22 5737-5742
DOI: 
https://doi.org/10.1073/pnas.1702188114
PubMed: 
28507142

Published By: 
National Academy of Sciences
Print ISSN: 
0027-8424
Online ISSN: 
1091-6490
History: 
  • Published in issue May 30, 2017.
  • Published first May 15, 2017.

Copyright & Usage: 
Freely available online through the PNAS open access option.

Author Information

  1. M. Scardiglia,b,
  2. C. Crocinia,b,
  3. C. Ferrantinic,
  4. T. Gabbriellia,
  5. L. Silvestria,b,
  6. R. Coppinid,
  7. C. Tesic,
  8. E. A. Rog-Zielinskae,f,
  9. P. Kohle,f,
  10. E. Cerbaid,
  11. C. Poggesic,
  12. F. S. Pavonea,b,g, and
  13. L. Sacconia,b,1
  1. aEuropean Laboratory for Non-Linear Spectroscopy, 50019 Sesto Fiorentino, Italy;
  2. bNational Institute of Optics, National Research Council, 50019 Sesto Fiorentino, Italy;
  3. cDivision of Physiology, Department of Experimental and Clinical Medicine, University of Florence, 50121 Florence, Italy;
  4. dDivision of Pharmacology, Dipartimento di Neuroscienze, Psicologia, Area del Farmaco e Salute del Bambino, University of Florence, 50139 Florence, Italy;
  5. eInstitute for Experimental Cardiovascular Medicine, University Heart Center Freiburg–Bad Krozingen, Faculty of Medicine, University of Freiburg, 79110 Freiburg, Germany;
  6. fThe Magdi Yacoub Institute, National Heart and Lung Institute, Imperial College London, UB9 6JH London, United Kingdom;
  7. gDepartment of Physics and Astronomy, University of Florence, 50019 Florence, Italy
  1. Edited by Clara Franzini-Armstrong, University of Pennsylvania Medical Center, Philadelphia, PA, and approved April 14, 2017 (received for review February 9, 2017)

Footnotes

  • ↵1To whom correspondence should be addressed. Email: sacconi{at}lens.unifi.it.
  • Author contributions: M.S., C.C., C.F., R.C., C.T., E.A.R.-Z., P.K., E.C., C.P., F.S.P., and L. Sacconi designed research; M.S. and L. Sacconi performed research; F.S.P. contributed new reagents/analytic tools; M.S., C.C., T.G., L. Silvestri, and L. Sacconi analyzed data; and M.S., C.C., C.F., T.G., L. Silvestri, R.C., C.T., E.A.R.-Z., P.K., E.C., C.P., and L. Sacconi wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1702188114/-/DCSupplemental.

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  • Solute movement in the t-tubule system of rabbit and mouse cardiomyocytes
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  • Interplay Between Sub-Cellular Alterations of Calcium Release and T-Tubular Defects in Cardiac Diseases
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  • Reply to Entcheva: The impact of T-tubules on action potential propagation in cardiac tissue
    M. Scardigli, C. Crocini, C. Ferrantini, T. Gabbrielli, L. Silvestri, R. Coppini, C. Tesi, E. A. Rog-Zielinska, P. Kohl, E. Cerbai, C. Poggesi, F. S. Pavone, L. Sacconi
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  • Species differences in the morphology of transverse tubule openings in cardiomyocytes
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Article usage

Article usage: May 2017 to February 2019

AbstractFullPdf
May 20173181158187
Jun 20172781120126
Jul 201717608370
Aug 20171103324
Sep 2017994229
Oct 20171012215
Nov 2017653014
Dec 201753189
Total 20178150506474
Jan 20181416539
Feb 2018555520
Mar 2018939830
Apr 2018515320
May 2018566313
Jun 201858647
Jul 2018394117
Aug 2018414612
Sep 20185313411
Oct 201877210
Nov 201818219
Dec 20180486
Total 2018595821204
Jan 201923211
Feb 20191145
Total 201934616
Total87481373694
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Assessment of electrical properties of TATS
M. Scardigli, C. Crocini, C. Ferrantini, T. Gabbrielli, L. Silvestri, R. Coppini, C. Tesi, E. A. Rog-Zielinska, P. Kohl, E. Cerbai, C. Poggesi, F. S. Pavone, L. Sacconi
Proceedings of the National Academy of Sciences May 2017, 114 (22) 5737-5742; DOI: 10.1073/pnas.1702188114

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Assessment of electrical properties of TATS
M. Scardigli, C. Crocini, C. Ferrantini, T. Gabbrielli, L. Silvestri, R. Coppini, C. Tesi, E. A. Rog-Zielinska, P. Kohl, E. Cerbai, C. Poggesi, F. S. Pavone, L. Sacconi
Proceedings of the National Academy of Sciences May 2017, 114 (22) 5737-5742; DOI: 10.1073/pnas.1702188114
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