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Research Article

Alignment of the protein substrate hairpin along the SecA two-helix finger primes protein transport in Escherichia coli

Qi Zhang, Sudipta Lahiri, Tithi Banerjee, Zhongmou Sun, Donald Oliver, and View ORCID ProfileIshita Mukerji
PNAS August 29, 2017 114 (35) 9343-9348; first published August 10, 2017; https://doi.org/10.1073/pnas.1702201114
Qi Zhang
aMolecular Biophysics Program, Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06459
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Sudipta Lahiri
aMolecular Biophysics Program, Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06459
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Tithi Banerjee
aMolecular Biophysics Program, Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06459
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Zhongmou Sun
aMolecular Biophysics Program, Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06459
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Donald Oliver
aMolecular Biophysics Program, Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06459
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Ishita Mukerji
aMolecular Biophysics Program, Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06459
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  • ORCID record for Ishita Mukerji
  • For correspondence: imukerji@wesleyan.edu
  1. Edited by Linda L. Randall, University of Missouri, Columbia, MO, and approved July 11, 2017 (received for review February 8, 2017)

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Article Information

vol. 114 no. 35 9343-9348
DOI: 
https://doi.org/10.1073/pnas.1702201114
PubMed: 
28798063

Published By: 
National Academy of Sciences
Print ISSN: 
0027-8424
Online ISSN: 
1091-6490
History: 
  • Published in issue August 29, 2017.
  • Published first August 10, 2017.

Copyright & Usage: 

Author Information

  1. Qi Zhanga,
  2. Sudipta Lahiria,
  3. Tithi Banerjeea,
  4. Zhongmou Suna,
  5. Donald Olivera, and
  6. Ishita Mukerjia,1
  1. aMolecular Biophysics Program, Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06459
  1. Edited by Linda L. Randall, University of Missouri, Columbia, MO, and approved July 11, 2017 (received for review February 8, 2017)

Footnotes

  • ↵1To whom correspondence should be addressed. Email: imukerji{at}wesleyan.edu.
  • Author contributions: Q.Z., D.O., and I.M. designed research; Q.Z., S.L., T.B., and Z.S. performed research; Q.Z. contributed new reagents/analytic tools; Q.Z., S.L., D.O., and I.M. analyzed data; and Q.Z., S.L., D.O., and I.M. wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1702201114/-/DCSupplemental.

Cited By...

  • 6 Citations
  • 6 Citations
  • Google Scholar

This article has been cited by the following articles in journals that are participating in Crossref Cited-by Linking.

  • ATP-induced asymmetric pre-protein folding as a driver of protein translocation through the Sec machinery
    Robin A Corey, Zainab Ahdash, Anokhi Shah, Euan Pyle, William J Allen, Tomas Fessl, Janet E Lovett, Argyris Politis, Ian Collinson
    eLife 2019 8
  • Driving Forces of Translocation Through Bacterial Translocon SecYEG
    Denis G. Knyazev, Roland Kuttner, Mirjam Zimmermann, Ekaterina Sobakinskaya, Peter Pohl
    The Journal of Membrane Biology 2018 251 3
  • MutSγ-Induced DNA Conformational Changes Provide Insights into Its Role in Meiotic Recombination
    Sudipta Lahiri, Yan Li, Manju M. Hingorani, Ishita Mukerji
    Biophysical Journal 2018 115 11
  • Motions of the SecA protein motor bound to signal peptide: Insights from molecular dynamics simulations
    Stefan Milenkovic, Ana-Nicoleta Bondar
    Biochimica et Biophysica Acta (BBA) - Biomembranes 2018 1860 2
  • Substrate Proteins Take Shape at an Improved Bacterial Translocon
    Donald Oliver, Thomas J. Silhavy
    Journal of Bacteriology 2018 201 1
  • Protein Secretion in Bacteria
    Amalina Ghaisani Komarudin, Arnold J. M. Driessen
    2019

Article usage

Article usage: August 2017 to January 2021

AbstractFullPdf
Aug 2017404793207
Sep 2017356759103
Oct 20176051917
Nov 20173471729
Dec 201714714
Total 20178713189360
Jan 20183131810
Feb 20184173714
Mar 2018805621
Apr 2018674625
May 2018695913
Jun 2018705711
Jul 201853355
Aug 201841273
Sep 201836355
Oct 20189303
Nov 201813714
Dec 20181705
Total 20181157507129
Jan 201910337
Feb 201917285
Mar 2019123316
Apr 201946414
May 20191247
Jun 20190217
Jul 201932913
Aug 201962512
Sep 20194144
Oct 20192275
Nov 20192204
Dec 20198157
Total 201969333101
Jan 20206232
Feb 20203208
Mar 202032914
Apr 202062615
May 20201205
Jun 20202117
Jul 20201201
Aug 20200197
Sep 20202243
Oct 202014187
Nov 202012356
Dec 202045112
Total 20205429687
Jan 20213175
Total 20213175
Total99961342682
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SecA two-helix finger primes protein transport
Qi Zhang, Sudipta Lahiri, Tithi Banerjee, Zhongmou Sun, Donald Oliver, Ishita Mukerji
Proceedings of the National Academy of Sciences Aug 2017, 114 (35) 9343-9348; DOI: 10.1073/pnas.1702201114

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SecA two-helix finger primes protein transport
Qi Zhang, Sudipta Lahiri, Tithi Banerjee, Zhongmou Sun, Donald Oliver, Ishita Mukerji
Proceedings of the National Academy of Sciences Aug 2017, 114 (35) 9343-9348; DOI: 10.1073/pnas.1702201114
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