Skip to main content
  • Submit
  • About
    • Editorial Board
    • PNAS Staff
    • FAQ
    • Rights and Permissions
    • Site Map
  • Contact
  • Journal Club
  • Subscribe
    • Subscription Rates
    • Subscriptions FAQ
    • Open Access
    • Recommend PNAS to Your Librarian
  • Log in
  • My Cart

Main menu

  • Home
  • Articles
    • Current
    • Latest Articles
    • Special Features
    • Colloquia
    • Collected Articles
    • PNAS Classics
    • Archive
  • Front Matter
  • News
    • For the Press
    • Highlights from Latest Articles
    • PNAS in the News
  • Podcasts
  • Authors
    • Information for Authors
    • Purpose and Scope
    • Editorial and Journal Policies
    • Submission Procedures
    • For Reviewers
    • Author FAQ
  • Submit
  • About
    • Editorial Board
    • PNAS Staff
    • FAQ
    • Rights and Permissions
    • Site Map
  • Contact
  • Journal Club
  • Subscribe
    • Subscription Rates
    • Subscriptions FAQ
    • Open Access
    • Recommend PNAS to Your Librarian

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Home
Home

Advanced Search

  • Home
  • Articles
    • Current
    • Latest Articles
    • Special Features
    • Colloquia
    • Collected Articles
    • PNAS Classics
    • Archive
  • Front Matter
  • News
    • For the Press
    • Highlights from Latest Articles
    • PNAS in the News
  • Podcasts
  • Authors
    • Information for Authors
    • Purpose and Scope
    • Editorial and Journal Policies
    • Submission Procedures
    • For Reviewers
    • Author FAQ

New Research In

Physical Sciences

Featured Portals

  • Physics
  • Chemistry
  • Sustainability Science

Articles by Topic

  • Applied Mathematics
  • Applied Physical Sciences
  • Astronomy
  • Computer Sciences
  • Earth, Atmospheric, and Planetary Sciences
  • Engineering
  • Environmental Sciences
  • Mathematics
  • Statistics

Social Sciences

Featured Portals

  • Anthropology
  • Sustainability Science

Articles by Topic

  • Economic Sciences
  • Environmental Sciences
  • Political Sciences
  • Psychological and Cognitive Sciences
  • Social Sciences

Biological Sciences

Featured Portals

  • Sustainability Science

Articles by Topic

  • Agricultural Sciences
  • Anthropology
  • Applied Biological Sciences
  • Biochemistry
  • Biophysics and Computational Biology
  • Cell Biology
  • Developmental Biology
  • Ecology
  • Environmental Sciences
  • Evolution
  • Genetics
  • Immunology and Inflammation
  • Medical Sciences
  • Microbiology
  • Neuroscience
  • Pharmacology
  • Physiology
  • Plant Biology
  • Population Biology
  • Psychological and Cognitive Sciences
  • Sustainability Science
  • Systems Biology

Fungal-induced protein hyperacetylation in maize identified by acetylome profiling

Justin W. Walley, Zhouxin Shen, Maxwell R. McReynolds, Eric A. Schmelz, and Steven P. Briggs
PNAS January 2, 2018 115 (1) 210-215; published ahead of print December 19, 2017 https://doi.org/10.1073/pnas.1717519115
Justin W. Walley
aDepartment of Plant Pathology and Microbiology, Iowa State University, Ames, IA 50011;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Justin W. Walley
  • For correspondence: jwalley@iastate.edusbriggs@ucsd.edu
Zhouxin Shen
bDivision of Biological Sciences, University of California, San Diego, La Jolla, CA 92093
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maxwell R. McReynolds
aDepartment of Plant Pathology and Microbiology, Iowa State University, Ames, IA 50011;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric A. Schmelz
bDivision of Biological Sciences, University of California, San Diego, La Jolla, CA 92093
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Steven P. Briggs
bDivision of Biological Sciences, University of California, San Diego, La Jolla, CA 92093
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: jwalley@iastate.edusbriggs@ucsd.edu
  1. Contributed by Steven P. Briggs, November 16, 2017 (sent for review October 18, 2017; reviewed by Alex Jones and Wenbo Ma)

  • Article
  • Figures & SI
  • Info & Metrics
  • PDF
Loading

Article Figures & SI

Figures

  • Fig. 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 1.

    Overview of treatments and proteome profiling. (A) Susceptible hm1A plants were exposed to a mock HCT solution or 100 μM HCT, HCT-deficient (Tox−), or HCT-producing (Tox+) strain of C. carbonum race 1. For each condition, four biological replicates were collected 22 h posttreatment to quantify protein abundance (iTRAQ) and acetylation levels (spectral counting). (B) Summary of sampled spectra, peptides, acetylated peptides, and identified proteins. Ac, acetylated; AcK, acetylated lysine; FDR, false discovery rate; NA, not applicable.

  • Fig. 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 2.

    HCT increases the levels of tryptophan biosynthetic proteins. (A) Overlap of protein abundance changes in response to treatments. (B) All detected proteins related to each step in tryptophan biosynthesis are shown. Heat maps represent the relative abundance of each protein following treatment by HCT, Tox−, or Tox+ relative to mock treatment.

  • Fig. 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 3.

    Dynamics of protein abundance and acetylation. (A) Spectral counts of the peptide corresponding to tetra-acetylation of H4 lysines 5, 8, 12, and 16. Data are means of four independent biological replicates ± SEM. (B) Western blot confirmation of H4 tetra-acetylation quantified by proteomics. (C) Overlap of acetylated peptides that change in response to treatments. (D) Number of acetylation sites that are altered, relative to mock treatment, by HCT, Tox−, or Tox+ treatment. (E) Number of GO categories related to transcriptional response, defense, or any other process (Other) that are overrepresented in HCT, Tox−, or Tox+ treatment. Reg., regulation.

  • Fig. 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 4.

    Diverse maize proteins are acetylated. Proteins from 32 of 35 major MapMan bins are acetylated.

Data supplements

  • Supporting Information

    • Download Supporting Information (PDF)
    • Download Dataset_S01 (XLSX)
    • Download Dataset_S02 (XLSX)
    • Download Dataset_S03 (XLSX)
PreviousNext
Back to top
Article Alerts
Email Article

Thank you for your interest in spreading the word on PNAS.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Fungal-induced protein hyperacetylation in maize identified by acetylome profiling
(Your Name) has sent you a message from PNAS
(Your Name) thought you would like to see the PNAS web site.
Citation Tools
Fungal-induced protein hyperacetylation
Justin W. Walley, Zhouxin Shen, Maxwell R. McReynolds, Eric A. Schmelz, Steven P. Briggs
Proceedings of the National Academy of Sciences Jan 2018, 115 (1) 210-215; DOI: 10.1073/pnas.1717519115

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
Fungal-induced protein hyperacetylation
Justin W. Walley, Zhouxin Shen, Maxwell R. McReynolds, Eric A. Schmelz, Steven P. Briggs
Proceedings of the National Academy of Sciences Jan 2018, 115 (1) 210-215; DOI: 10.1073/pnas.1717519115
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Mendeley logo Mendeley
Proceedings of the National Academy of Sciences: 116 (7)
Current Issue

Submit

Sign up for Article Alerts

Jump to section

  • Article
    • Abstract
    • Results
    • Discussion
    • Materials and Methods
    • Acknowledgments
    • Footnotes
    • References
  • Figures & SI
  • Info & Metrics
  • PDF

You May Also be Interested in

Several aspects of the proposal, which aims to expand open access, require serious discussion and, in some cases, a rethink.
Opinion: “Plan S” falls short for society publishers—and for the researchers they serve
Several aspects of the proposal, which aims to expand open access, require serious discussion and, in some cases, a rethink.
Image credit: Dave Cutler (artist).
Several large or long-lived animals seem strangely resistant to developing cancer. Elucidating the reasons why could lead to promising cancer-fighting strategies in humans.
Core Concept: Solving Peto’s Paradox to better understand cancer
Several large or long-lived animals seem strangely resistant to developing cancer. Elucidating the reasons why could lead to promising cancer-fighting strategies in humans.
Image credit: Shutterstock.com/ronnybas frimages.
Featured Profile
PNAS Profile of NAS member and biochemist Hao Wu
 Nonmonogamous strawberry poison frog (Oophaga pumilio).  Image courtesy of Yusan Yang (University of Pittsburgh, Pittsburgh).
Putative signature of monogamy
A study suggests a putative gene-expression hallmark common to monogamous male vertebrates of some species, namely cichlid fishes, dendrobatid frogs, passeroid songbirds, common voles, and deer mice, and identifies 24 candidate genes potentially associated with monogamy.
Image courtesy of Yusan Yang (University of Pittsburgh, Pittsburgh).
Active lifestyles. Image courtesy of Pixabay/MabelAmber.
Meaningful life tied to healthy aging
Physical and social well-being in old age are linked to self-assessments of life worth, and a spectrum of behavioral, economic, health, and social variables may influence whether aging individuals believe they are leading meaningful lives.
Image courtesy of Pixabay/MabelAmber.

More Articles of This Classification

Biological Sciences

  • Structural basis for activity of TRIC counter-ion channels in calcium release
  • PGC1A regulates the IRS1:IRS2 ratio during fasting to influence hepatic metabolism downstream of insulin
  • Altered neural odometry in the vertical dimension
Show more

Plant Biology

  • Mitochondrial small heat shock protein mediates seed germination via thermal sensing
  • Enhanced resistance to bacterial and oomycete pathogens by short tandem target mimic RNAs in tomato
  • North America’s oldest boreal trees are more efficient water users due to increased [CO2], but do not grow faster
Show more

Related Content

  • No related articles found.
  • Scopus
  • PubMed
  • Google Scholar

Cited by...

  • RAMOSA1 ENHANCER LOCUS2-Mediated Transcriptional Repression Regulates Vegetative and Reproductive Architecture
  • Scopus (7)
  • Google Scholar

Similar Articles

Site Logo
Powered by HighWire
  • Submit Manuscript
  • Twitter
  • Facebook
  • RSS Feeds
  • Email Alerts

Articles

  • Current Issue
  • Latest Articles
  • Archive

PNAS Portals

  • Classics
  • Front Matter
  • Teaching Resources
  • Anthropology
  • Chemistry
  • Physics
  • Sustainability Science

Information

  • Authors
  • Editorial Board
  • Reviewers
  • Press
  • Site Map

Feedback    Privacy/Legal

Copyright © 2019 National Academy of Sciences. Online ISSN 1091-6490