Fungal-induced protein hyperacetylation in maize identified by acetylome profiling

Justin W. Walley, Zhouxin Shen, Maxwell R. McReynolds, Eric A. Schmelz, and Steven P. Briggs
PNAS January 2, 2018 115 (1) 210-215; published ahead of print December 19, 2017 https://doi.org/10.1073/pnas.1717519115

  1. Contributed by Steven P. Briggs, November 16, 2017 (sent for review October 18, 2017; reviewed by Alex Jones and Wenbo Ma)

Article Figures & SI

Figures

  • Fig. 1.
    Fig. 1.

    Overview of treatments and proteome profiling. (A) Susceptible hm1A plants were exposed to a mock HCT solution or 100 μM HCT, HCT-deficient (Tox−), or HCT-producing (Tox+) strain of C. carbonum race 1. For each condition, four biological replicates were collected 22 h posttreatment to quantify protein abundance (iTRAQ) and acetylation levels (spectral counting). (B) Summary of sampled spectra, peptides, acetylated peptides, and identified proteins. Ac, acetylated; AcK, acetylated lysine; FDR, false discovery rate; NA, not applicable.

  • Fig. 2.
    Fig. 2.

    HCT increases the levels of tryptophan biosynthetic proteins. (A) Overlap of protein abundance changes in response to treatments. (B) All detected proteins related to each step in tryptophan biosynthesis are shown. Heat maps represent the relative abundance of each protein following treatment by HCT, Tox−, or Tox+ relative to mock treatment.

  • Fig. 3.
    Fig. 3.

    Dynamics of protein abundance and acetylation. (A) Spectral counts of the peptide corresponding to tetra-acetylation of H4 lysines 5, 8, 12, and 16. Data are means of four independent biological replicates ± SEM. (B) Western blot confirmation of H4 tetra-acetylation quantified by proteomics. (C) Overlap of acetylated peptides that change in response to treatments. (D) Number of acetylation sites that are altered, relative to mock treatment, by HCT, Tox−, or Tox+ treatment. (E) Number of GO categories related to transcriptional response, defense, or any other process (Other) that are overrepresented in HCT, Tox−, or Tox+ treatment. Reg., regulation.

  • Fig. 4.
    Fig. 4.

    Diverse maize proteins are acetylated. Proteins from 32 of 35 major MapMan bins are acetylated.

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Fungal-induced protein hyperacetylation
Justin W. Walley, Zhouxin Shen, Maxwell R. McReynolds, Eric A. Schmelz, Steven P. Briggs
Proceedings of the National Academy of Sciences Jan 2018, 115 (1) 210-215; DOI: 10.1073/pnas.1717519115
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