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Research Article

Matching material and cellular timescales maximizes cell spreading on viscoelastic substrates

Ze Gong, Spencer E. Szczesny, Steven R. Caliari, Elisabeth E. Charrier, Ovijit Chaudhuri, Xuan Cao, Yuan Lin, Robert L. Mauck, Paul A. Janmey, Jason A. Burdick, and Vivek B. Shenoy
PNAS March 20, 2018 115 (12) E2686-E2695; first published March 5, 2018; https://doi.org/10.1073/pnas.1716620115
Ze Gong
aDepartment of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104;
bDepartment of Mechanical Engineering, University of Hong Kong, Hong Kong, China;
cCenter for Engineering Mechanobiology, University of Pennsylvania, Philadelphia, PA 19104;
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Spencer E. Szczesny
dMcKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104;
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Steven R. Caliari
eDepartment of Chemical Engineering, University of Virginia, Charlottesville, VA 22904;
fDepartment of Biomedical Engineering, University of Virginia, Charlottesville, VA 22904;
gDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104;
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Elisabeth E. Charrier
hInstitute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104;
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Ovijit Chaudhuri
iDepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305
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Xuan Cao
aDepartment of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104;
cCenter for Engineering Mechanobiology, University of Pennsylvania, Philadelphia, PA 19104;
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Yuan Lin
bDepartment of Mechanical Engineering, University of Hong Kong, Hong Kong, China;
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  • For correspondence: vshenoy@seas.upenn.edu ylin@hku.hk
Robert L. Mauck
cCenter for Engineering Mechanobiology, University of Pennsylvania, Philadelphia, PA 19104;
dMcKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104;
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Paul A. Janmey
cCenter for Engineering Mechanobiology, University of Pennsylvania, Philadelphia, PA 19104;
hInstitute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104;
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Jason A. Burdick
cCenter for Engineering Mechanobiology, University of Pennsylvania, Philadelphia, PA 19104;
gDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104;
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Vivek B. Shenoy
aDepartment of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104;
cCenter for Engineering Mechanobiology, University of Pennsylvania, Philadelphia, PA 19104;
gDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104;
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  • For correspondence: vshenoy@seas.upenn.edu ylin@hku.hk
  1. Edited by David A. Weitz, Harvard University, Cambridge, MA, and approved February 5, 2018 (received for review September 21, 2017)

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Article Information

vol. 115 no. 12 E2686-E2695
DOI: 
https://doi.org/10.1073/pnas.1716620115
PubMed: 
29507238

Published By: 
National Academy of Sciences
Print ISSN: 
0027-8424
Online ISSN: 
1091-6490
History: 
  • Published in issue March 20, 2018.
  • Published first March 5, 2018.

Article Versions

  • Previous version (March 5, 2018 - 12:33).
  • You are viewing the most recent version of this article.
Copyright & Usage: 
© 2018 Published under the PNAS license.

Author Information

  1. Ze Gonga,b,c,
  2. Spencer E. Szczesnyd,
  3. Steven R. Caliarie,f,g,
  4. Elisabeth E. Charrierh,
  5. Ovijit Chaudhurii,
  6. Xuan Caoa,c,
  7. Yuan Linb,1,
  8. Robert L. Mauckc,d,
  9. Paul A. Janmeyc,h,
  10. Jason A. Burdickc,g, and
  11. Vivek B. Shenoya,c,g,1
  1. aDepartment of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104;
  2. bDepartment of Mechanical Engineering, University of Hong Kong, Hong Kong, China;
  3. cCenter for Engineering Mechanobiology, University of Pennsylvania, Philadelphia, PA 19104;
  4. dMcKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104;
  5. eDepartment of Chemical Engineering, University of Virginia, Charlottesville, VA 22904;
  6. fDepartment of Biomedical Engineering, University of Virginia, Charlottesville, VA 22904;
  7. gDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104;
  8. hInstitute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104;
  9. iDepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305
  1. Edited by David A. Weitz, Harvard University, Cambridge, MA, and approved February 5, 2018 (received for review September 21, 2017)

Footnotes

  • ↵1To whom correspondence may be addressed. Email: vshenoy{at}seas.upenn.edu or ylin{at}hku.hk.
  • Author contributions: V.B.S. designed research; Z.G., S.R.C., E.E.C., and O.C. performed research; Z.G., S.E.S., X.C., R.L.M., P.A.J., and J.A.B. analyzed data; Z.G., S.E.S., Y.L., and V.B.S. wrote the paper; and S.R.C., E.E.C., and O.C. conducted the experiments.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

  • This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1716620115/-/DCSupplemental.

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Mar 20183667491383
Apr 2018855204144
May 201866310496
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Jul 20189696244
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Total 2018844917241159
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Total 201911661343806
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Jul 20205214079
Aug 20209114185
Sep 20206216293
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Total 20209101673953
Jan 2021309333
Total 2021309333
Total1055548332951
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Matching material and cellular timescales maximizes cell spreading on viscoelastic substrates
Ze Gong, Spencer E. Szczesny, Steven R. Caliari, Elisabeth E. Charrier, Ovijit Chaudhuri, Xuan Cao, Yuan Lin, Robert L. Mauck, Paul A. Janmey, Jason A. Burdick, Vivek B. Shenoy
Proceedings of the National Academy of Sciences Mar 2018, 115 (12) E2686-E2695; DOI: 10.1073/pnas.1716620115

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Matching material and cellular timescales maximizes cell spreading on viscoelastic substrates
Ze Gong, Spencer E. Szczesny, Steven R. Caliari, Elisabeth E. Charrier, Ovijit Chaudhuri, Xuan Cao, Yuan Lin, Robert L. Mauck, Paul A. Janmey, Jason A. Burdick, Vivek B. Shenoy
Proceedings of the National Academy of Sciences Mar 2018, 115 (12) E2686-E2695; DOI: 10.1073/pnas.1716620115
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