BIOCHEMISTRY Correction for “A mitochondrial ferredoxin is essential for biogenesis of cellular iron-sulfur proteins,” by Heike Lange, Anita Kaut, Gyula Kispal, and Roland Lill, which was first published February 1, 2000; 10.1073/pnas.97.3.1050 (Proc Natl Acad Sci USA 97:1050–1055).
The authors wish to note the following: “Recently when we reanalyzed Fig. 4B (a Western blot visualizing the yeast protein Leu1), we noticed that there was an error in the assembly of the bands. Because the gel loading for the Western blot did not fit the desired order for presenting the data, the first author cut the thermoprinter paper printout into pieces and reassembled them. Unfortunately, the published version contained an unintentional data duplication (mirrored bands for the 64 h data points), but the overall results were not impacted. We apologize for the mistake.” The corrected Fig. 4 and its legend appear below.
Mitochondrial ferredoxin Yah1p is required for the activity of the cytosolic Fe/S protein Leu1p. (A) Wild-type (WT) cells were grown in minimal medium in the presence of galactose (Gal) or glucose (Glu). For depletion of Yah1p, the Gal-YAH1 cells were cultivated in minimal medium in the presence of glucose for the indicated times. A cell lysate was prepared by breaking the cells with glass beads. The enzyme activities of isopropyl malate isomerase (Leu1p) were measured immediately (29). (B) Aliquots of the cell extracts obtained as described in A were analyzed for the amount of Leu1p by immunostaining.
Published under the PNAS license.
