Osmotic stabilization prevents cochlear synaptopathy after blast trauma

Blast Exposure Produces Endolymphatic Hydrops.

We delivered a single blast wave to an anesthetized adult mouse and then serially imaged the cochlear apical turn in vivo using optical coherence tomography (OCT) (18⇓–20) (Fig. 1 C and D and Movie S1). All three scalae—Reissner’s membrane, the basilar membrane (BM), and the tectorial membrane (TM)—could be resolved. During the first 3 h after the blast, there was progressive bulging of Reissner’s membrane, consistent with an increase in endolymph volume, termed endolymphatic hydrops (21) (Fig. 1E and Movie S2). Nine hours after the blast, the position of Reissner’s membrane began to normalize (Movie S3). By 1 d after the blast, Reissner’s membrane returned to a normal position, where it remained throughout the 7-d period we studied (Fig. 1 F–H).

In contrast, unexposed control mice demonstrated no changes in the position of Reissner’s membrane (Fig. 1I), indicating that the imaging procedure does not cause endolymphatic hydrops. Following death, Reissner’s membrane progressively shifted inward (Fig. 1J), consistent with reduced endolymph secretion by the stria vascularis postmortem (16, 22). We then quantified endolymph volume within scala media and perilymph volume within scala vestibuli (Fig. 1K and Fig. S1). This confirmed that these fluid volumes changed dynamically and in opposite directions.

Loss of Outer Hair Cells After Blast Exposure.

To assess for permanent injury, we counted hair cells 7 d after the blast. This confirmed the pattern of basal outer hair cell (OHC) loss with no loss of inner hair cells (IHCs) (Fig. 2 A and B). We then immunolabeled hair cells for myosin VIIa within the basal cochlear epithelium at various time points during the first 24 h (Fig. 2 C–F). Mice killed immediately following blast exposure exhibited no morphological evidence of OHC damage. Mice killed after 1 h demonstrated clumping of myosin VIIa within some OHCs, consistent with cellular degradation. This was more prevalent 3 h after blast exposure. Mice killed after 1 d demonstrated no clumping but did have substantial OHC loss. Thus, the number of OHCs that were missing or damaged increased over the 7 d following blast exposure (Fig. 2G).

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Fig. 2.

Blast exposure causes stereociliary trauma, hair cell loss, and cochlear synaptopathy. (A) The cochlear epithelium 7 d after blast exposure. Immunolabeling was done to visualize all hair cells (myosin VIIa) and outer hair cells (OHCs) (Prestin). Representative locations of missing hair cells are shown (arrowheads). (B) Quantification of OHC loss 7 d after blast exposure and TUNEL⁺ cells right after blast exposure in the apical, middle, and basal regions. (C–F) Representative examples of the cochlear epithelium from the basal region immediately following blast exposure (0 h) and at several time points afterward. (G) Progressive OHC loss in the cochlear base. (H–L) The cochlear epithelium harvested from a mouse immediately following blast exposure (H–J), from an unexposed mouse (negative control, K), and from an unexposed mouse in which DNase I was applied to the cochlea (positive control, L). The TUNEL assay labels cells with fragmented DNA, which detects apoptotic programmed cell death. (M and N) Scanning electron microscopic images of OHC stereociliary bundles in representative control and blast-exposed mice. (O and P) The cochlear epithelium 7 d after blast exposure. Immunolabeling was done to visualize synaptic ribbons in hair cells (CtBP2), hair cells (myosin VIIa), and auditory neurons (Tuj1). (Q and R) Quantification of synaptic ribbons per OHC and IHC in control and blast-exposed mice. **P < 0.01, ***P < 0.001.

To characterize DNA fragmentation, a hallmark of apoptosis, we performed the TUNEL assay on the basal cochlear epithelium (Fig. 2 H–L). The percentages of TUNEL⁺ OHCs immediately following the blast were nearly identical to the percentages of OHC loss 7 d after the blast (Fig. 2B). This indicates that OHC fate was determined by the initial trauma of the blast, not from secondary factors arising later, such as endolymphatic hydrops.

Because the stereociliary bundle is the most sensitive mechanical component of the cochlea, OHC death may be initiated by stereociliary trauma. Scanning electron microscopy of basal OHCs from unexposed control mice (n = 3) exhibited normal bundle organization (Fig. 2M). The stereocilia within each bundle were evenly spaced and had an equal height within each row. In contrast, mice killed immediately after blast exposure (n = 3) had distorted bundle morphology (Fig. 2N). Many stereocilia were fused together or bent. Some regions of the bundles were missing stereocilia. Thus, blast exposure appeared to cause immediate stereociliary damage.

Cochlear Synaptopathy After Blast Exposure.

To assess for hair cell–auditory neuron synapse damage, we counted the number of synaptic ribbons per residual OHC and IHC 7 d after blast exposure by immunolabeling for CtBP2, a marker for the presynaptic hair cell ribbon (Fig. 2 O and P). Throughout the cochlea, there were significant reductions in the number of synaptic ribbons per OHC and IHC (Fig. 2 Q and R), although we could not study the IHCs in the base because their vertical orientation made it hard to consistently acquire the high-quality images necessary to count synaptic ribbons.

There was no TUNEL labeling of spiral ganglion neurons within cross-sections of cochleae harvested immediately and 1 d after blast exposure, nor in unexposed controls (n = 4 for each case) (Fig. S2). Thus, although hair cell fate is set immediately following the blast, spiral ganglion neuron fate is not.

Perilymph Does Not Mix with Endolymph After Blast Exposure.

OHCs that die could create holes in the epithelium. If the surrounding cells take longer to deform and seal the defect than it takes for the OHCs to be evacuated, perilymph might enter scala media and produce endolymphatic hydrops (23⇓–25). To test this hypothesis, we rapidly perfused gold nanoparticles throughout the perilymph. We performed serial imaging to localize the nanoparticles during the perfusion and for 30 min afterward. Because both endolymphatic hydrops and OHC degeneration were obvious 3 h after the blast, we chose this time point to start the experiment.

The perfused gold nanoparticles filled both scala vestibuli and scala tympani, whereas scala media remained clear in blast-exposed and control mice (Fig. 3 and Movies S4 and S5). To quantify this, both cohorts demonstrated large increases in signal intensity within scala vestibuli. However, no significant changes were found within scala media for either cohort. These data argue that posttraumatic endolymphatic hydrops does not result from defects in the endolymph–perilymph barrier larger than 50 nm, consistent with the concept that swelling of the Deiters cell phalangeal processes seal defects in the reticular lamina when OHCs die (23).

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Fig. 3.

Perilymph and endolymph do not mix after blast exposure. Gold nanoparticles (GNP) were perfused through the perilymph. (A) OCT images of a representative unexposed control mouse 3 h after performing the surgical exposure (A) and 30 min after GNP perfusion (A′). (B) OCT images in a representative mouse 3 h after blast exposure (B) and 30 min after GNP perfusion (B′). In both conditions, GNP could be detected within the perilymphatic chambers (SV and ST, arrowheads) but not within endolymphatic (SM). (C) Measurement of the pixel intensity of discrete regions within SV and SM (yellow boxes) before and 30 min after the perfusion. The percent change in pixel intensity was calculated by normalizing the change in pixel intensity to the pixel intensity before perfusion. *P < 0.05.

Stereociliary Trauma Is the First Step in the Development of Blast-Induced Cochlear Trauma.

Next, we examined Tecta^{C1509G/C1509G} mice, in which the TM does not deflect the OHC stereocilia because it is detached from the epithelium (26). Previously, we found that 2 h of noise exposure did not cause hair cell loss in this mouse strain, whereas it did in controls (14). Similarly, we found no hair cell loss 7 d after blast exposure (Fig. S3). Furthermore, there was no change in endolymph volume after blast exposure (Fig. 4 A–C). These data indicate that blast-induced stereociliary trauma occurs because the bundle is sheared by the TM, and that, in our animal model, this trauma initiates the damage cascade that leads to hair cell loss and endolymphatic hydrops.

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Fig. 4.

Stereociliary trauma and endolymphatic K⁺ are necessary for the development of endolymphatic hydrops after blast exposure. (A) A representative unexposed Tecta^{C1509G/C1509G} mouse demonstrates a stable, but increased, volume of endolymph. (B) A representative Tecta^{C1509G/C1509G} mouse after blast exposure. (C) Endolymph volume was higher than normal, yet unchanged by blast-exposure in Tecta^{C1509G/C1509G} mice. (D) Postulated mechanism of endolymph volume dysregulation in Tecta^{C1509G/C1509G} mice. The TM is elevated off the epithelium and so does not cause any static displacement of the OHC stereociliary bundles. Thus, K⁺ bias currents through transduction channels (red X) are reduced, increasing endolymph [K⁺]. H₂O enters endolymph because of the increased osmotic load. (E) A representative unexposed Tyr-DT-A mouse demonstrates a stable, but decreased, volume of endolymph. (F) A representative Tyr-DT-A mouse after blast exposure. (G) Endolymph volume in Tyr-DT-A mice was lower than normal, yet unchanged, by blast exposure. (H) Postulated mechanism of endolymph volume dysregulation in Tyr-DT-A mice. K⁺ secretion from the stria vascularis is blocked (red X), reducing endolymph [K⁺]. H₂O leaves endolymph because the osmotic load is now higher in perilymph. (I) A representative CBA mouse that was dosed with furosemide and then exposed to a blast. (J) Endolymph volume did not increase after blast exposure in mice pretreated with furosemide. (K) Postulated mechanism of endolymph volume dysregulation in CBA mice after blast exposure. Damaged stereocilia and OHC loss (red X) reduce K⁺ bias currents through transduction channels (red X), increasing endolymph [K⁺] and drawing in H₂O. (L) With furosemide treatment, K⁺ secretion by the stria is also blocked, so endolymphatic [K⁺] is expected to remain stable. ***P < 0.001.

Osmotic Gradients Modulate Endolymph Volume.

Endolymphatic [K⁺] depends on K⁺ entry and exit rates; this may affect the osmotic load and thereby modulate endolymph volume (27⇓⇓–30). For example, death inhibits K⁺ secretion (22), which correlates with reduced endolymph volume. Furthermore, stereociliary damage after traumatic exposure impairs transduction, reduces K⁺ bias currents (31), and correlates with increased endolymph volume. Compared with unexposed controls, unexposed Tecta^{C1509G/C1509G} mice had distention of Reissner’s membrane and increased endolymph volume (P = 0.002) (compare Figs. 1K and 4C). This is consistent with reduced K⁺ bias currents through transduction channels because of the lack of a static bundle deflection in Tecta mutant mice (26, 32, 33) (Fig. 4D).

To better define the role of K⁺ in modulating endolymph volume, we studied Tyr-DT-A mice, in which ablation of stria intermediate cells abolishes K⁺ secretion (34) (Fig. 4 E–H). Unexposed Tyr-DT-A mice demonstrated an inward deflection of Reissner’s membrane and reduced endolymph volume compared with controls (P = 0.007), which did not change after blast. As confirmation, we injected CBA mice just before blast exposure with furosemide to inhibit the strial Na-K-Cl cotransporter NKCC2 that secretes K⁺. Endolymph volume remained stable after blast exposure in these mice (Fig. 4 I–L). Together, these data argue that K⁺ is required to develop blast-induced endolymphatic hydrops.

We directly tested whether changing perilymph tonicity affects endolymph volume by perfusing artificial perilymph in control CBA mice over 3 min (Fig. 5 A–C). Consistent with this prediction, Reissner’s membrane bowed outward with hypotonic, remained in a normal position with normotonic, and bowed inward with hypertonic perilymph. Perilymphatic perfusion requires opening the cochlea, risking hearing loss, and so this technique is not used clinically for inner ear drug delivery. However, round window application is safe (35⇓–37), and we found that this clinical technique also works with osmotic challenge (Fig. 5 D–I). Reissner’s membrane shifted in accordance with the applied tonicity and the effect did not depend upon the solute used. However, osmotic gradients >300 mM were required to see displacements in Reissner’s membrane with round window application, whereas gradients of only 10 mM were needed with perilymphatic perfusion. Regardless, these data demonstrate that osmotic gradients between endolymph and perilymph modulate endolymph volume and these effects are not ion-specific.

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Fig. 5.

Osmotic treatment of endolymphatic hydrops partially rescues synaptic ribbon loss after blast exposure. (A–C) Perilymph osmolality modulates endolymph volume. Perfusion of hypotonic (A), normotonic (B), or hypertonic (C) artificial perilymph dynamically shifted the position of Reissner’s membrane (arrowheads). This occurred rapidly (<3 min). (D) Simple model of noninvasive osmotic challenge via the round window membrane (RWM) to alter the volume of endolymph and shift Reissner’s membrane (RM). Hypotonic solution applied to the middle ear causes endolymphatic hydrops, whereas hypertonic solution reduces endolymphatic volume. (E–I) The application of solutions of varying osmotic loads into the middle ear adjacent to the round window modulates endolymph volume. Representative OCT images were taken before and 30 min after applying the osmotic challenge. Hypotonic challenge (E) increased endolymph volume, normotonic saline (F) caused no change in endolymph volume, and hypertonic challenges (G–I) decreased endolymph volume. (J and K) Representative mice were treated with normotonic or hypertonic challenge 3 h after blast exposure. Repeat images were taken 30-min later. (L) Normotonic artificial perilymph had no impact on posttraumatic endolymphatic hydrops, whereas hypertonic artificial perilymph normalized endolymphatic volume. (M–O) The cochlear epithelium from representative mice 2 mo after blast exposure. Mice had either no treatment (M), normotonic artificial perilymph application to the middle ear after the blast (N), or hypertonic artificial perilymph application to the middle ear after the blast (O). Immunolabeling was done to visualize synaptic ribbons in hair cells (CtBP2), hair cells (myosin VIIa), and auditory neurons (Tuj1). (P–R) Quantification of OHC loss and synaptic ribbons per OHC and IHC. Hypertonic artificial perilymph reduced the loss of synaptic ribbons but did not affect the degree of OHC loss. **P < 0.01, ***P < 0.001.

Hypertonic Challenge Reverses Endolymphatic Hydrops and Rescues Synaptic Ribbon Loss.

We then tested whether osmotic challenge modulates cochlear blast trauma by applying artificial perilymph to the round window 3 h after blast exposure. Normotonic perilymph had no effect, whereas hypertonic perilymph reversed the posttraumatic endolymphatic hydrops (Fig. 5 J–L).

Because OHC fate was set immediately by the blast trauma, we did not expect that treating endolymphatic hydrops would rescue hair cells. However, treating endolymphatic hydrops may alter cochlear synaptopathy. For example, patients with Meniere’s disease, who have endolymphatic hydrops, have afferent neuron loss beyond that predicted by damage to hair cells (38, 39). This finding has been replicated in animal models of endolymphatic hydrops (40, 41).

We therefore performed a blinded, randomized, prospective study comparing normotonic versus hypertonic artificial perilymph treatment of blast-exposed CBA mice. Thirty minutes after blast exposure, the treatment solution was applied to the left round window. The untreated right ear was a control. Another control group consisted of age-matched mice that were not blast-exposed. Two months later, we counted OHCs and synaptic ribbons (Fig. 5 M–O).

While unexposed control mice had no OHC loss, untreated ears from blast-exposed mice had substantial loss of OHCs in the base, scattered loss in the middle, and no loss in the apex. Hair cell counts were no different between the untreated ears and those treated with normotonic or hypertonic artificial perilymph (Fig. 5P). Compared with unexposed control mice, there were 36–51% reductions in the number of synaptic ribbons per OHC and IHC in untreated ears and in ears treated with normotonic artificial perilymph (Fig. 5 Q and R). However, treatment with hypertonic artificial perilymph prevented 45–64% of this loss. Thus, hypertonic treatment of posttraumatic endolymphatic hydrops preserved roughly half the synaptic ribbons that would have been lost without treatment, but had no impact on OHC loss.

As confirmation, we performed a second blinded, randomized, prospective study, but instead tested normotonic and hypertonic NaCl solutions rather than artificial perilymph. Hair cell and synaptic ribbon counts 7 d after the blast exposure were consistent with those of the first trial (Fig. S4).

Auditory Physiology After Blast Exposure.

To assess whether hypertonic treatment preserves cochlear function after blast trauma, we measured auditory brainstem responses (ABRs) and distortion product otoacoustic emissions (DPOAEs) during the first trial (Fig. S5 A–J). ABR thresholds partially recovered and stabilized 1 mo after the blast exposure, whereas DPOAE thresholds demonstrated no meaningful recovery. There were no differences in average threshold recovery between the cohorts. We also measured the peak-to-peak of ABR wave 1 for all stimulus intensities 2 mo after the blast exposure (Fig. S6 A–C); however, there were no differences between the three cohorts. We then further analyzed this data by normalizing the peak-to-peak value at a suprathreshold levels [70-dB sound-pressure level (SPL)] to the preblast values (Fig. S6D), but again there were no differences. This surprised us because this measurement has been shown to correlate with cochlear synaptopathy, even with OHC loss (42).

To understand this result, we measured mechanical responses of the BMs and TMs to sound. Control mice demonstrated compressive nonlinearities consistent with cochlear amplification (20, 43) with ∼100-nm peak vibratory responses (Fig. 6 A–C) (n = 3). However, blast-exposed mice demonstrated significant variability (Fig. S5 K–P). Some mice responded with compressive nonlinearities, some had passive responses, indicating no cochlear amplification, and some demonstrated no vibration at all. A consistent finding, however, was that none of the blast-exposed mice had peak vibratory responses larger than 10 nm. These findings argue that the blast exposure caused variable degrees of cochlear, and possibly also middle ear, damage (13). Therefore, improvements in ABR wave I responses that theoretically should result with hypertonic treatment are masked by the large variability in damage patterns.

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Fig. 6.

Endolymphatic hydrops correlates with cochlear synaptopathy, but not loss of hair cells or cochlear amplification. (A–C) A representative control mouse with a normal amount of endolymph had normal nonlinear vibratory responses measured from the BM and TM. (D–F) A representative mouse with endolymphatic hydrops (arrowhead) induced by 2 h of noise exposure. Vibratory responses were linear, consistent with loss of cochlear amplification. (G–I) A representative mouse with endolymphatic hydrops (arrowhead) induced by the application of hypotonic challenge to the middle ear. Vibratory responses for both the BM and TM were normal. (J) The cochlear epithelium harvested from a mouse with endolymphatic hydrops induced by hypotonic challenge to the middle ear. (K and L) Endolymphatic hydrops caused a loss of synaptic ribbons similar to that found after blast exposure. *P < 0.05, **P < 0.01, ***P < 0.001. (M–O) The cochlear epithelium harvested from representative mice perfused for 1 h with 304 mOsm (M), 294 mOsm (N), or 294 mOsm + 100 µM CNQX (O) artificial perilymph. Immunolabeling was done to visualize presynaptic ribbons in IHCs (CtBP2) and postsynaptic auditory nerve boutons (Homer). Colocalization of the pre- and postsynaptic terminals is shown (Insets). (P) Hypotonic challenge reduced CtBP2 and Homer counts. Blocking glutamate with CNQX preserved both the pre- and postsynaptic terminals. *P < 0.05; ns, not significant. (Q) Hypotonic challenge reduced the rate of CtBP2 and Homer colocalization; this effect was also inhibited with CNQX. (R) Flowchart detailing our postulated pathophysiological sequence of cochlear damage after blast exposure. The colored text reflects previously published findings [blue (4, 5), violet (6⇓–8)]. Hypertonic challenge blocks both endolymphatic hydrops and swelling of the synaptic boutons (red arrows).

How Does Endolymphatic Hydrops Adversely Affect the Cochlea?

To remove these confounding effects of variability in the trauma, we studied mice exposed to band-pass–filtered white noise. Similar noise protocols produce minimal or no OHC loss in the base and a reduction of synaptic ribbons in residual hair cells throughout the cochlea (11, 14). In contrast to control mice that had sharp tuning and large gains of cochlear amplification (BM: 62.3 ± 0.6 dB, TM: 65.3 ± 0.5 dB, n = 3, 11 kHz), mice exposed to noise developed endolymphatic hydrops and vibratory responses with broad tuning and little gain (Fig. 6 D–F) (BM: 9.2 ± 1.2 dB, TM: 17.9 ± 2.7, P < 0.001 for both comparisons, n = 3, 5 kHz). Thus, noise trauma clearly impacted OHC function but the role of endolymphatic hydrops in causing this effect remained unclear.

Next, we induced endolymphatic hydrops in mice without noise trauma by applying a hypotonic challenge to the round window for 5.5 h. These mice had vibratory response curves and gains similar to controls (Fig. 6 G–I) (BM: 59.3 ± 1.0 dB, TM: 60.3 ± 2.5 dB, n = 4, 11 kHz, P = 0.06 and 0.12). Therefore, isolated endolymphatic hydrops (i.e., not associated with mechanical trauma of stereociliary bundles) does not alter OHC based cochlear amplification. However, synaptic ribbon counts were reduced after the hypotonic challenge to a level similar to that found after blast exposure, even though there was no loss of OHCs (n = 4) (Fig. 6 J–L). Thus, endolymphatic hydrops in isolation causes cochlear synaptopathy.

Finally, we studied both the pre- and postsynaptic sides of the IHC synapse after perilymphatic perfusion of either normotonic or hypotonic artificial perilymph over 1 h by also immunolabeling postsynaptic densities with Homer (44) (Fig. 6 M–P). Mice perfused with hypotonic perilymph had lower CtBP2 and Homer puncta counts than those perfused with normotonic perilymph. However, blocking the AMPA glutamate receptor with CNQX preserved both CtBP2 and Homer puncta counts. The rates of CtBP2/Homer puncta colocalization were reduced with hypotonic perfusion but preserved with the addition of CNQX (Fig. 6Q). Thus, hypotonic challenge induces glutamate secretion that not only damages both sides of synaptic cleft but also disrupts pre- and postsynaptic pairing.