Table of Contents

Biological Sciences

• Biochemistry

  • You have access

    Formylglycine-generating enzyme binds substrate directly at a mononuclear Cu(I) center to initiate O₂ activation

    Mason J. Appel, Katlyn K. Meier, Julien Lafrance-Vanasse, Hyeongtaek Lim, Chi-Lin Tsai, Britt Hedman, Keith O. Hodgson, John A. Tainer, Edward I. Solomon, and Carolyn R. Bertozzi

    PNAS March 19, 2019 116 (12) 5370-5375; first published March 1, 2019. https://doi.org/10.1073/pnas.1818274116
  • Open Access

    HDAC11 regulates type I interferon signaling through defatty-acylation of SHMT2

    Ji Cao, Lei Sun, Pornpun Aramsangtienchai, Nicole A. Spiegelman, Xiaoyu Zhang, Weishan Huang, Edward Seto, and Hening Lin

    PNAS March 19, 2019 116 (12) 5487-5492; first published February 28, 2019. https://doi.org/10.1073/pnas.1815365116
  • Open Access

    Cryo-electron microscopy structure of the filamentous bacteriophage IKe

    Jingwei Xu, Nir Dayan, Amir Goldbourt, and Ye Xiang

    PNAS March 19, 2019 116 (12) 5493-5498; first published February 28, 2019. https://doi.org/10.1073/pnas.1811929116
  • You have access

    Genome mining and biosynthesis of a polyketide from a biofertilizer fungus that can facilitate reductive iron assimilation in plant

    Mengbin Chen, Qikun Liu, Shu-Shan Gao, Abbegayle E. Young, Steven E. Jacobsen, and Yi Tang

    PNAS March 19, 2019 116 (12) 5499-5504; first published March 6, 2019. https://doi.org/10.1073/pnas.1819998116
  • You have access

    Stepwise 5′ DNA end-specific resection of DNA breaks by the Mre11-Rad50-Xrs2 and Sae2 nuclease ensemble

    Elda Cannavo, Giordano Reginato, and Petr Cejka

    PNAS March 19, 2019 116 (12) 5505-5513; first published February 28, 2019. https://doi.org/10.1073/pnas.1820157116
  • You have access

    Interleukin-37 monomer is the active form for reducing innate immunity

    Elan Z. Eisenmesser, Adrian Gottschlich, Jasmina S. Redzic, Natasia Paukovich, Jay C. Nix, Tania Azam, Lingdi Zhang, Rui Zhao, Jeffrey S. Kieft, Erlinda The, Xianzhong Meng, and Charles A. Dinarello

    PNAS March 19, 2019 116 (12) 5514-5522; first published February 28, 2019. https://doi.org/10.1073/pnas.1819672116

• Biophysics and Computational Biology

  • You have access

    Domain topology, stability, and translation speed determine mechanical force generation on the ribosome

    Sarah E. Leininger, Fabio Trovato, Daniel A. Nissley, and Edward P. O’Brien

    PNAS March 19, 2019 116 (12) 5523-5532; first published March 1, 2019. https://doi.org/10.1073/pnas.1813003116
  • Open Access

    Spastin is a dual-function enzyme that severs microtubules and promotes their regrowth to increase the number and mass of microtubules

    Yin-Wei Kuo, Olivier Trottier, Mohammed Mahamdeh, and Jonathon Howard

    PNAS March 19, 2019 116 (12) 5533-5541; first published March 5, 2019. https://doi.org/10.1073/pnas.1818824116
  • You have access

    Evolutionarily informed deep learning methods for predicting relative transcript abundance from DNA sequence

    Jacob D. Washburn, Maria Katherine Mejia-Guerra, Guillaume Ramstein, Karl A. Kremling, Ravi Valluru, Edward S. Buckler, and Hai Wang

    PNAS March 19, 2019 116 (12) 5542-5549; first published March 6, 2019. https://doi.org/10.1073/pnas.1814551116

• Cell Biology

  • Open Access

    COX-2 mediates tumor-stromal prolactin signaling to initiate tumorigenesis

    • Inaugural Article

    Yu Zheng, Valentine Comaills, Risa Burr, Gaylor Boulay, David T. Miyamoto, Ben S. Wittner, Erin Emmons, Srinjoy Sil, Michael W. Koulopoulos, Katherine T. Broderick, Eric Tai, Shruthi Rengarajan, Anupriya S. Kulkarni, Toshi Shioda, Chin-Lee Wu, Sridhar Ramaswamy, David T. Ting, Mehmet Toner, Miguel N. Rivera, Shyamala Maheswaran, and Daniel A. Haber

    PNAS March 19, 2019 116 (12) 5223-5232; first published February 28, 2019. https://doi.org/10.1073/pnas.1819303116

    See companion article:

    • Profile of Daniel A. Haber

      - Mar 11, 2019
  • Open Access

    Encoding biological recognition in a bicomponent cell-membrane mimic

    Cesar Rodriguez-Emmenegger, Qi Xiao, Nina Yu. Kostina, Samuel E. Sherman, Khosrow Rahimi, Benjamin E. Partridge, Shangda Li, Dipankar Sahoo, Aracelee M. Reveron Perez, Irene Buzzacchera, Hong Han, Meir Kerzner, Ishita Malhotra, Martin Möller, Christopher J. Wilson, Matthew C. Good, Mark Goulian, Tobias Baumgart, Michael L. Klein, and Virgil Percec

    PNAS March 19, 2019 116 (12) 5376-5382; first published February 28, 2019. https://doi.org/10.1073/pnas.1821924116
  • Open Access

    Motional dynamics of single Patched1 molecules in cilia are controlled by Hedgehog and cholesterol

    Lucien E. Weiss, Ljiljana Milenkovic, Joshua Yoon, Tim Stearns, and W. E. Moerner

    PNAS March 19, 2019 116 (12) 5550-5557; first published February 28, 2019. https://doi.org/10.1073/pnas.1816747116
  • Open Access

    Double-negative T cells remarkably promote neuroinflammation after ischemic stroke

    Hailan Meng, Haoran Zhao, Xiang Cao, Junwei Hao, He Zhang, Yi Liu, Min-sheng Zhu, Lizhen Fan, Leihua Weng, Lai Qian, Xiaoying Wang, and Yun Xu

    PNAS March 19, 2019 116 (12) 5558-5563; first published February 28, 2019. https://doi.org/10.1073/pnas.1814394116
  • Open Access

    Infection by the parasitic helminth Trichinella spiralis activates a Tas2r-mediated signaling pathway in intestinal tuft cells

    Xiao-Cui Luo, Zhen-Huang Chen, Jian-Bo Xue, Dong-Xiao Zhao, Chen Lu, Yi-Hong Li, Song-Min Li, Ya-Wen Du, Qun Liu, Ping Wang, Mingyuan Liu, and Liquan Huang

    PNAS March 19, 2019 116 (12) 5564-5569; first published February 28, 2019. https://doi.org/10.1073/pnas.1812901116

• Developmental Biology

  • Open Access

    Theory of mechanochemical patterning in biphasic biological tissues

    Pierre Recho, Adrien Hallou, and Edouard Hannezo

    PNAS March 19, 2019 116 (12) 5344-5349; first published February 28, 2019. https://doi.org/10.1073/pnas.1813255116
  • You have access

    Statistics of noisy growth with mechanical feedback in elastic tissues

    Ojan Khatib Damavandi and David K. Lubensky

    PNAS March 19, 2019 116 (12) 5350-5355; first published February 28, 2019. https://doi.org/10.1073/pnas.1816100116

    See companion article:

    • Modulation of tissue growth heterogeneity by responses to mechanical stress

      - Jan 23, 2019
  • You have access

    Neural crest-derived neurons invade the ovary but not the testis during mouse gonad development

    Jennifer McKey, Corey Bunce, Iordan S. Batchvarov, David M. Ornitz, and Blanche Capel

    PNAS March 19, 2019 116 (12) 5570-5575; first published February 28, 2019. https://doi.org/10.1073/pnas.1814930116

• Ecology

  • You have access

    Seedling traits predict drought-induced mortality linked to diversity loss

    Susan Harrison and Marina LaForgia

    PNAS March 19, 2019 116 (12) 5576-5581; first published March 4, 2019. https://doi.org/10.1073/pnas.1818543116
  • You have access

    Memory and resource tracking drive blue whale migrations

    • From the Cover

    Briana Abrahms, Elliott L. Hazen, Ellen O. Aikens, Matthew S. Savoca, Jeremy A. Goldbogen, Steven J. Bograd, Michael G. Jacox, Ladd M. Irvine, Daniel M. Palacios, and Bruce R. Mate

    PNAS March 19, 2019 116 (12) 5582-5587; first published February 25, 2019. https://doi.org/10.1073/pnas.1819031116

    See companion article:

    • Migrating whales depend on memory to exploit reliable resources

      - Feb 25, 2019
  • Open Access

    Narrow safety margin in the phyllosphere during thermal extremes

    • From the Cover

    Sylvain Pincebourde and Jérôme Casas

    PNAS March 19, 2019 116 (12) 5588-5596; first published February 19, 2019. https://doi.org/10.1073/pnas.1815828116

    See companion article:

    • In a globally warming world, insects act locally to manipulate their own microclimate

      - Mar 06, 2019
  • Open Access

    Oxytocin/vasopressin-like peptide inotocin regulates cuticular hydrocarbon synthesis and water balancing in ants

    Akiko Koto, Naoto Motoyama, Hiroki Tahara, Sean McGregor, Minoru Moriyama, Takayoshi Okabe, Masayuki Miura, and Laurent Keller

    PNAS March 19, 2019 116 (12) 5597-5606; first published March 6, 2019. https://doi.org/10.1073/pnas.1817788116

• Environmental Sciences

  • You have access

    Localized prosocial preferences, public goods, and common-pool resources

    Andrew R. Tilman, Avinash K. Dixit, and Simon A. Levin

    PNAS March 19, 2019 116 (12) 5305-5310; first published October 8, 2018. https://doi.org/10.1073/pnas.1802872115
  • You have access

    The blue paradox: Preemptive overfishing in marine reserves

    Grant R. McDermott, Kyle C. Meng, Gavin G. McDonald, and Christopher J. Costello

    PNAS March 19, 2019 116 (12) 5319-5325; first published August 27, 2018. https://doi.org/10.1073/pnas.1802862115

    See companion article:

    • Reply to Hanich et al.: Alternate explanations for the blue paradox do not withstand statistical scrutiny

      - Dec 17, 2018
  • You have access

    Jet stream dynamics, hydroclimate, and fire in California from 1600 CE to present

    Eugene R. Wahl, Eduardo Zorita, Valerie Trouet, and Alan H. Taylor

    PNAS March 19, 2019 116 (12) 5393-5398; first published March 4, 2019. https://doi.org/10.1073/pnas.1815292116

• Evolution

  • You have access

    Evolution of nitric oxide regulation of gut function

    Junko Yaguchi and Shunsuke Yaguchi

    PNAS March 19, 2019 116 (12) 5607-5612; first published March 4, 2019. https://doi.org/10.1073/pnas.1816973116
  • Open Access

    Environment-dependent fitness gains can be driven by horizontal gene transfer of transporter-encoding genes

    David S. Milner, Victoria Attah, Emily Cook, Finlay Maguire, Fiona R. Savory, Mark Morrison, Carolin A. Müller, Peter G. Foster, Nicholas J. Talbot, Guy Leonard, and Thomas A. Richards

    PNAS March 19, 2019 116 (12) 5613-5622; first published March 6, 2019. https://doi.org/10.1073/pnas.1815994116
  • Open Access

    Weevil pgrp-lb prevents endosymbiont TCT dissemination and chronic host systemic immune activation

    Justin Maire, Carole Vincent-Monégat, Séverine Balmand, Agnès Vallier, Mireille Hervé, Florent Masson, Nicolas Parisot, Aurélien Vigneron, Caroline Anselme, Jackie Perrin, Julien Orlans, Isabelle Rahioui, Pedro Da Silva, Marie-Odile Fauvarque, Dominique Mengin-Lecreulx, Anna Zaidman-Rémy, and Abdelaziz Heddi

    PNAS March 19, 2019 116 (12) 5623-5632; first published February 28, 2019. https://doi.org/10.1073/pnas.1821806116
  • Open Access

    Regulatory changes in pterin and carotenoid genes underlie balanced color polymorphisms in the wall lizard

    • From the Cover

    Pedro Andrade, Catarina Pinho, Guillem Pérez i de Lanuza, Sandra Afonso, Jindřich Brejcha, Carl-Johan Rubin, Ola Wallerman, Paulo Pereira, Stephen J. Sabatino, Adriana Bellati, Daniele Pellitteri-Rosa, Zuzana Bosakova, Ignas Bunikis, Miguel A. Carretero, Nathalie Feiner, Petr Marsik, Francisco Paupério, Daniele Salvi, Lucile Soler, Geoffrey M. While, Tobias Uller, Enrique Font, Leif Andersson, and Miguel Carneiro

    PNAS March 19, 2019 116 (12) 5633-5642; first published February 28, 2019. https://doi.org/10.1073/pnas.1820320116
  • Open Access

    The genetic architecture of teosinte catalyzed and constrained maize domestication

    Chin Jian Yang, Luis Fernando Samayoa, Peter J. Bradbury, Bode A. Olukolu, Wei Xue, Alessandra M. York, Michael R. Tuholski, Weidong Wang, Lora L. Daskalska, Michael A. Neumeyer, Jose de Jesus Sanchez-Gonzalez, Maria Cinta Romay, Jeffrey C. Glaubitz, Qi Sun, Edward S. Buckler, James B. Holland, and John F. Doebley

    PNAS March 19, 2019 116 (12) 5643-5652; first published March 6, 2019. https://doi.org/10.1073/pnas.1820997116

• Genetics

  • Open Access

    Patterns of genome-wide allele-specific expression in hybrid rice and the implications on the genetic basis of heterosis

    Lin Shao, Feng Xing, Conghao Xu, Qinghua Zhang, Jian Che, Xianmeng Wang, Jiaming Song, Xianghua Li, Jinghua Xiao, Ling-Ling Chen, Yidan Ouyang, and Qifa Zhang

    PNAS March 19, 2019 116 (12) 5653-5658; first published March 4, 2019. https://doi.org/10.1073/pnas.1820513116
  • You have access

    A SIR-independent role for cohesin in subtelomeric silencing and organization

    Deepash Kothiwal and Shikha Laloraya

    PNAS March 19, 2019 116 (12) 5659-5664; first published March 6, 2019. https://doi.org/10.1073/pnas.1816582116
  • Open Access

    Chloroplast competition is controlled by lipid biosynthesis in evening primroses

    Johanna Sobanski, Patrick Giavalisco, Axel Fischer, Julia M. Kreiner, Dirk Walther, Mark Aurel Schöttler, Tommaso Pellizzer, Hieronim Golczyk, Toshihiro Obata, Ralph Bock, Barbara B. Sears, and Stephan Greiner

    PNAS March 19, 2019 116 (12) 5665-5674; first published March 4, 2019. https://doi.org/10.1073/pnas.1811661116

• Immunology and Inflammation

  • You have access

    RIP1 kinase inhibitor halts the progression of an immune-induced demyelination disease at the stage of monocyte elevation

    Sitao Zhang, Yaning Su, Zhengxin Ying, Dejia Guo, Chenjie Pan, Jia Guo, Ziye Zou, Lei Wang, Ze Zhang, Zhaodi Jiang, Zhiyuan Zhang, and Xiaodong Wang

    PNAS March 19, 2019 116 (12) 5675-5680; first published March 5, 2019. https://doi.org/10.1073/pnas.1819917116
  • Open Access

    Renal control of disease tolerance to malaria

    Susana Ramos, Ana Rita Carlos, Balamurugan Sundaram, Viktoria Jeney, Ana Ribeiro, Raffaella Gozzelino, Claudia Bank, Erida Gjini, Faouzi Braza, Rui Martins, Temitope Wilson Ademolue, Birte Blankenhaus, Zélia Gouveia, Pedro Faísca, Damian Trujillo, Sílvia Cardoso, Sofia Rebelo, Laura del Barrio, Abolfazl Zarjou, Subhashini Bolisetty, Anupam Agarwal, and Miguel P. Soares

    PNAS March 19, 2019 116 (12) 5681-5686; first published March 4, 2019. https://doi.org/10.1073/pnas.1822024116

• Medical Sciences

  • You have access

    MDA-7/IL-24 regulates the miRNA processing enzyme DICER through downregulation of MITF

    Anjan K. Pradhan, Praveen Bhoopathi, Sarmistha Talukdar, Danielle Scheunemann, Devanand Sarkar, Webster K. Cavenee, Swadesh K. Das, Luni Emdad, and Paul B. Fisher

    PNAS March 19, 2019 116 (12) 5687-5692; first published March 6, 2019. https://doi.org/10.1073/pnas.1819869116

• Microbiology

  • Open Access

    Human cytomegalovirus haplotype reconstruction reveals high diversity due to superinfection and evidence of within-host recombination

    Juliana Cudini, Sunando Roy, Charlotte J. Houldcroft, Josephine M. Bryant, Daniel P. Depledge, Helena Tutill, Paul Veys, Rachel Williams, Austen J. J. Worth, Asif U. Tamuri, Richard A. Goldstein, and Judith Breuer

    PNAS March 19, 2019 116 (12) 5693-5698; first published February 28, 2019. https://doi.org/10.1073/pnas.1818130116

    See companion article:

    • Reply to Jensen and Kowalik: Consideration of mixed infections is central to understanding HCMV intrahost diversity

      - Dec 24, 2019
  • Open Access

    NF-κB activation is a turn on for vaccinia virus phosphoprotein A49 to turn off NF-κB activation

    Sarah Neidel, Hongwei Ren, Alice A. Torres, and Geoffrey L. Smith

    PNAS March 19, 2019 116 (12) 5699-5704; first published February 28, 2019. https://doi.org/10.1073/pnas.1813504116
  • You have access

    TIM-mediated inhibition of HIV-1 release is antagonized by Nef but potentiated by SERINC proteins

    Minghua Li, Abdul A. Waheed, Jingyou Yu, Cong Zeng, Hui-Yu Chen, Yi-Min Zheng, Amin Feizpour, Björn M. Reinhard, Suryaram Gummuluru, Steven Lin, Eric O. Freed, and Shan-Lu Liu

    PNAS March 19, 2019 116 (12) 5705-5714; first published March 6, 2019. https://doi.org/10.1073/pnas.1819475116

• Neuroscience

  • You have access

    Activity-dependent visualization and control of neural circuits for courtship behavior in the fly Drosophila melanogaster

    Seika Takayanagi-Kiya and Taketoshi Kiya

    PNAS March 19, 2019 116 (12) 5715-5720; first published March 5, 2019. https://doi.org/10.1073/pnas.1814628116
  • You have access

    PERIOD-controlled deadenylation of the timeless transcript in the Drosophila circadian clock

    Brigitte Grima, Christian Papin, Béatrice Martin, Elisabeth Chélot, Prishila Ponien, Eric Jacquet, and François Rouyer

    PNAS March 19, 2019 116 (12) 5721-5726; first published March 4, 2019. https://doi.org/10.1073/pnas.1814418116
  • You have access

    MicroRNA-186-5p controls GluA2 surface expression and synaptic scaling in hippocampal neurons

    Mariline M. Silva, Beatriz Rodrigues, Joana Fernandes, Sandra D. Santos, Laura Carreto, Manuel A. S. Santos, Paulo Pinheiro, and Ana Luísa Carvalho

    PNAS March 19, 2019 116 (12) 5727-5736; first published February 26, 2019. https://doi.org/10.1073/pnas.1900338116
  • You have access

    Long-term population spike-timing-dependent plasticity promotes synaptic tagging but not cross-tagging in rat hippocampal area CA1

    Karen Ka Lam Pang, Mahima Sharma, Kumar Krishna-K., Thomas Behnisch, and Sreedharan Sajikumar

    PNAS March 19, 2019 116 (12) 5737-5746; first published February 28, 2019. https://doi.org/10.1073/pnas.1817643116
  • You have access

    Transcranial alternating current stimulation entrains single-neuron activity in the primate brain

    Matthew R. Krause, Pedro G. Vieira, Bennett A. Csorba, Praveen K. Pilly, and Christopher C. Pack

    PNAS March 19, 2019 116 (12) 5747-5755; first published March 4, 2019. https://doi.org/10.1073/pnas.1815958116

    See companion article:

    • Reply to Khatoun et al.: Speculation about brain stimulation must be constrained by observation

      - Oct 15, 2019
  • Open Access

    Control of hearing sensitivity by tectorial membrane calcium

    Clark Elliott Strimbu, Sonal Prasad, Pierre Hakizimana, and Anders Fridberger

    PNAS March 19, 2019 116 (12) 5756-5764; first published March 5, 2019. https://doi.org/10.1073/pnas.1805223116
  • Open Access

    Parkinson’s disease-linked D620N VPS35 knockin mice manifest tau neuropathology and dopaminergic neurodegeneration

    Xi Chen, Jennifer K. Kordich, Erin T. Williams, Nathan Levine, Allyson Cole-Strauss, Lee Marshall, Viviane Labrie, Jiyan Ma, Jack W. Lipton, and Darren J. Moore

    PNAS March 19, 2019 116 (12) 5765-5774; first published March 6, 2019. https://doi.org/10.1073/pnas.1814909116
  • You have access

    Lipid transporter TMEM24/C2CD2L is a Ca²⁺-regulated component of ER–plasma membrane contacts in mammalian neurons

    Elizabeth Wen Sun, Andrés Guillén-Samander, Xin Bian, Yumei Wu, Yiying Cai, Mirko Messa, and Pietro De Camilli

    PNAS March 19, 2019 116 (12) 5775-5784; first published February 28, 2019. https://doi.org/10.1073/pnas.1820156116
  • Open Access

    AAV cis-regulatory sequences are correlated with ocular toxicity

    Wenjun Xiong, David M. Wu, Yunlu Xue, Sean K. Wang, Michelle J. Chung, Xuke Ji, Parimal Rana, Sophia R. Zhao, Shuyi Mai, and Constance L. Cepko

    PNAS March 19, 2019 116 (12) 5785-5794; first published March 4, 2019. https://doi.org/10.1073/pnas.1821000116

• Plant Biology

  • You have access

    Constitutive signaling activity of a receptor-associated protein links fertilization with embryonic patterning in Arabidopsis thaliana

    Ancilla Neu, Emily Eilbert, Lisa Y. Asseck, Daniel Slane, Agnes Henschen, Kai Wang, Patrick Bürgel, Melanie Hildebrandt, Thomas J. Musielak, Martina Kolb, Wolfgang Lukowitz, Christopher Grefen, and Martin Bayer

    PNAS March 19, 2019 116 (12) 5795-5804; first published March 4, 2019. https://doi.org/10.1073/pnas.1815866116

• Psychological and Cognitive Sciences

  • You have access

    Symbolic labeling in 5-month-old human infants

    Claire Kabdebon and Ghislaine Dehaene-Lambertz

    PNAS March 19, 2019 116 (12) 5805-5810; first published March 5, 2019. https://doi.org/10.1073/pnas.1809144116
  • Open Access

    Task-driven visual exploration at the foveal scale

    Natalya Shelchkova, Christie Tang, and Martina Poletti

    PNAS March 19, 2019 116 (12) 5811-5818; first published March 1, 2019. https://doi.org/10.1073/pnas.1812222116

• Sustainability Science

  • You have access

    Causal inference in coupled human and natural systems

    Paul J. Ferraro, James N. Sanchirico, and Martin D. Smith

    PNAS March 19, 2019 116 (12) 5311-5318; first published August 20, 2018. https://doi.org/10.1073/pnas.1805563115

• Systems Biology

  • Open Access

    Genomic and molecular characterization of preterm birth

    Theo A. Knijnenburg, Joseph G. Vockley, Nyasha Chambwe, David L. Gibbs, Crystal Humphries, Kathi C. Huddleston, Elisabeth Klein, Prachi Kothiyal, Ryan Tasseff, Varsha Dhankani, Dale L. Bodian, Wendy S. W. Wong, Gustavo Glusman, Denise E. Mauldin, Michael Miller, Joseph Slagel, Summer Elasady, Jared C. Roach, Roger Kramer, Kalle Leinonen, Jasper Linthorst, Rajiv Baveja, Robin Baker, Benjamin D. Solomon, Greg Eley, Ramaswamy K. Iyer, George L. Maxwell, Brady Bernard, Ilya Shmulevich, Leroy Hood, and John E. Niederhuber

    PNAS March 19, 2019 116 (12) 5819-5827; first published March 4, 2019. https://doi.org/10.1073/pnas.1716314116