Correction for Cardol et al., Impaired respiration discloses the physiological significance of state transitions in Chlamydomonas

PLANT BIOLOGY Correction for “Impaired respiration discloses the physiological significance of state transitions in Chlamydomonas,” by Pierre Cardol, Jean Alric, Jacqueline Girard-Bascou, Fabrice Franck, Francis-André Wollman, and Giovanni Finazzi, which was first published September 1, 2009; 10.1073/pnas.0908111106 (Proc Natl Acad Sci USA 106:15979–15984).

The authors wish to note the following: “In the original Fig. 1A, there had been a mistake while assembling the image. Wrong spots have been selected to illustrate similar growth of the WT and stt7-9 mutant strains in minimal (‘Min’) medium. This error led to duplicating the spot corresponding the growth of WT in presence of the respiration inhibitor myxothiazol (‘Min + M’). As seen in the new Fig. 1A, this mistake in mounting the figure does not change the results nor the conclusion, i.e., stt7-9 mutant impaired in state transitions hardly grow when its respiratory activity is prevented by addition of myxothiazol, an inhibitor of the respiratory chain.

• Download figure
• Open in new tab
• Download powerpoint

Fig. 1.

Impact of inhibition of the mitochondrial respiratory chain on growth of ST-deficient strain. Cells were cultivated at 50 μmol photons m⁻²s⁻¹ in mixotrophic (TAP) or photoautotrophic (Min) conditions in the absence or presence (+M) of the mitochondrial respiratory inhibitor myxothiazol (5 μM). (A) Drops of cell suspensions (≈0.01 nm A₇₅₀) were plated on solid media, and growth was estimated after 3–5 days. (B) Doubling time in liquid cultures. Error bars indicate SD of the mean of 3 independent measurements. (C) The experimental procedure is the same as in A. Photoautrotrophic (Min) conditions. Light intensity was either 50 or 400 μmol photons m⁻²s⁻¹.

“Additionally, two mistakes occurred in the final assembly of Fig. S1: spot A7 (Min plate) and H8 (TAP plate) have been duplicated as spot F5 (TAP plate) and spot F5 (Min plate), respectively. The original data are no longer available to update the image. This mistake in mounting the figure does not change the conclusion of this work, i.e., all double mutants dum22 stt7-9 (including F5) have a severe phenotype and hardly grow.”

The corrected Fig. 1 and its legend appear below. The online version has been corrected.