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Research Article

Short and simple sequences favored the emergence of N-helix phospho-ligand binding sites in the first enzymes

Liam M. Longo, View ORCID ProfileDušan Petrović, View ORCID ProfileShina Caroline Lynn Kamerlin, and Dan S. Tawfik
  1. aDepartment of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel;
  2. bScience for Life Laboratory, Department of Chemistry-BMC, Uppsala University, S-751 23 Uppsala, Sweden

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PNAS March 10, 2020 117 (10) 5310-5318; first published February 20, 2020; https://doi.org/10.1073/pnas.1911742117
Liam M. Longo
aDepartment of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel;
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Dušan Petrović
bScience for Life Laboratory, Department of Chemistry-BMC, Uppsala University, S-751 23 Uppsala, Sweden
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  • ORCID record for Dušan Petrović
Shina Caroline Lynn Kamerlin
bScience for Life Laboratory, Department of Chemistry-BMC, Uppsala University, S-751 23 Uppsala, Sweden
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  • ORCID record for Shina Caroline Lynn Kamerlin
Dan S. Tawfik
aDepartment of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel;
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  • For correspondence: dan.tawfik@weizmann.ac.il
  1. Edited by William F. DeGrado, University of California, San Francisco, CA, and approved January 25, 2020 (received for review July 10, 2019)

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Article Information

vol. 117 no. 10 5310-5318
DOI: 
https://doi.org/10.1073/pnas.1911742117
PubMed: 
32079722

Published By: 
National Academy of Sciences
Print ISSN: 
0027-8424
Online ISSN: 
1091-6490
History: 
  • Published in issue March 10, 2020.
  • Published first February 20, 2020.

Article Versions

  • Previous version (February 20, 2020 - 11:32).
  • You are viewing the most recent version of this article.
Copyright & Usage: 
© 2020 Published under the PNAS license.

Author Information

  1. Liam M. Longoa,
  2. Dušan Petrovićb,1,
  3. Shina Caroline Lynn Kamerlinb, and
  4. Dan S. Tawfika,2
  1. aDepartment of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel;
  2. bScience for Life Laboratory, Department of Chemistry-BMC, Uppsala University, S-751 23 Uppsala, Sweden
  1. Edited by William F. DeGrado, University of California, San Francisco, CA, and approved January 25, 2020 (received for review July 10, 2019)

Footnotes

  • ↵1Present address: Hit Discovery, Discovery Sciences, BioPharmaceuticals R&D, AstraZeneca, 431 83 Gothenburg, Sweden.

  • ↵2To whom correspondence may be addressed. Email: dan.tawfik{at}weizmann.ac.il.
  • Author contributions: L.M.L., D.P., S.C.L.K., and D.S.T. designed research; L.M.L. and D.P. performed research; L.M.L., D.P., S.C.L.K., and D.S.T. analyzed data; and L.M.L. and D.S.T. wrote the paper with feedback from D.P. and S.C.L.K.

  • The authors declare no competing interest.

  • This article is a PNAS Direct Submission.

  • This article contains supporting information online at https://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1911742117/-/DCSupplemental.

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    Journal of the American Chemical Society 2020 142 26
  • Nonenzymatic Metabolic Reactions and Life’s Origins
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    Chemical Reviews 2020 120 15
  • On the emergence of P-Loop NTPase and Rossmann enzymes from a Beta-Alpha-Beta ancestral fragment
    Liam M Longo, Jagoda Jabłońska, Pratik Vyas, Manil Kanade, Rachel Kolodny, Nir Ben-Tal, Dan S Tawfik
    eLife 2020 9
  • Cofactors as Molecular Fossils To Trace the Origin and Evolution of Proteins
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    ChemBioChem 2020 21 22
  • A Completely De Novo ATPase from Combinatorial Protein Design
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    Journal of the American Chemical Society 2020 142 36
  • Polyamines Mediate Folding of Primordial Hyperacidic Helical Proteins
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Article usage

Article usage: February 2020 to March 2021

AbstractFullPdf
Feb 20201267190103
Mar 2020149111882
Apr 20201543823
May 20201843225
Jun 20201463225
Jul 20201382327
Aug 20201475925
Sep 2020159130
Oct 20203210927
Nov 20203511124
Dec 20204311541
Total 20203652918432
Jan 2021216726
Feb 2021167626
Mar 20213194
Total 20214016256
Total36921080488
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Short and simple sequences favored the emergence of N-helix phospho-ligand binding sites in the first enzymes
Liam M. Longo, Dušan Petrović, Shina Caroline Lynn Kamerlin, Dan S. Tawfik
Proceedings of the National Academy of Sciences Mar 2020, 117 (10) 5310-5318; DOI: 10.1073/pnas.1911742117

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Short and simple sequences favored the emergence of N-helix phospho-ligand binding sites in the first enzymes
Liam M. Longo, Dušan Petrović, Shina Caroline Lynn Kamerlin, Dan S. Tawfik
Proceedings of the National Academy of Sciences Mar 2020, 117 (10) 5310-5318; DOI: 10.1073/pnas.1911742117
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